Abstract
In vitro caffeine contracture tests were carried out on whole rat muscle composed primarily of either histochemical type I or type II fibers. Muscles composed primarily of type I fibers developed contractures at lower concentrations of caffeine and had lower caffeine specific concentrations than muscles composed primarily of type II fibers. These findings indicate that the histochemical profile of a muscle can influence the results of the in-vitro caffeine contracture test.
Résumé
Le but de ce travail était d’évaluer l’influence du caractère histochimique des muscles utilisés dans les tests de contractures à la caféine in vitro, selon que des fibres musculaires de type I ou de type II sont utilisées. A cette fin nous avons effectué des montages à partir de muscles entiers de l’un et de l’autre type, prélevés chez le rat. Les contractures survenaient à des concentrations de caféine moindres dans le cas des fibres de type I et les concentrations spécifiques de caféine (mM de caféine requises pour élever d’un gramme la tension du muscle) étaient également inférieures dans le cas de fibres de type I que dans celui des fibres de type II. Ces résultats indiquent que le profil histochimique du muscle utilisé peut modifier les résultats des tests de contractures à la caféine effectués in vitro.
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This research was supported by a grant from the Medical Services Research Foundation of Alberta.
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Brownell, A.K.W., Szabo, M. The in-vitro caffeine contracture test: Influence of the muscle histochemical profile on test results. Can. Anaesth. Soc. J. 29, 218–221 (1982). https://doi.org/10.1007/BF03007119
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DOI: https://doi.org/10.1007/BF03007119