Abstract
The analgesic effectiveness of various local anaesthetic solutions was measured in the distribution of the infraorbital nerve of the rat. Using Sprague-Dawley rats sedated with phenobarbitone 25mg/kg intraperitoneally and a simple stereotactic technique, 0.2 ml of solution was deposited at the infraorbital notch. The onset and duration of analgesia were ascertained by timing the unilateral absence of aversive response to pinching the upper lip, tested at intervals of five minutes. The experiments were performed with coded solutions, the entire code being preserved intact until completion of the study. Solutions were tested on sets of eight animals weighing 500–600g. The average duration (minutes ± S.D.) of analgesia produced by the respective injectates was as follows:
Lidocaine 0.5% | -42 ±12 | Procaine 1.5% | - 46± 13 |
Lidocaine 1.0% | -47±10 | 2-Chloroprocaine 1.5% | - 38±9 |
Mepivacaine 0.5% | -58± 13 | Bupivacaine 0.5% | -100±40 |
Mepivacaine 1.0% | - 78 ± 23 | Etidocaine 1.0% | - 59 ±25 |
Procaine 0.5% | - 0 ± 0 | Tetracaine 0.2% | - 0 ± 0 |
Procaine 1.0% | -34±10 | Tetracaine 0.3% | - 0± 0 |
Comparison with other animal models used to evaluate conduction block indicates that this technique affords an improved, standardized and reliable experimental guide to the clinical analgesic properties of local anaesthetic agents.
Résumé
Ľefficacité analgésique de quelques solutions et concentrations ďanesthésiques locaux a été évaluée dans le territoire de distribution du nerf infraorbital. Chez des rats de race Sprague-Dawley sous sédation au phénobarbitone à la dose de 25mg · kg-1 administrée par voie intrapéritonéale et sous contrôle stéréotaxique simple, on a injecté une solution de 0.2 ml au niveau de ľéchancrure infraorbitale. Le début et la durée de ľanalgésie ont été mesurés en chronométrant la disparition unilatérale de la réaction ďaversion provoquée par le pincement de la lèvre inférieure recherchée aux cinq minutes. On a utilisé des solutions codées dont le secret a été gardé jusqu’à la fin de ľétude. Les tests ont été effectués sur des séries de huit animaux pesant de 500 à 600g. La durée moyenne (en minutes ± E.T.) ďanalgésie produite par les injections ont été comme suit:
Lidocaine 0.5% | -42 ±12 | Procaine 1.5% | - 46± 13 |
Lidocaine 1.0% | -47±10 | 2-Chloroprocaine 1.5% | - 38±9 |
Mepivacaine 0.5% | -58± 13 | Bupivacaine 0.5% | -100±40 |
Mepivacaine 1.0% | - 78 ± 23 | Etidocaine 1.0% | - 59 ±25 |
Procaine 0.5% | - 0 ± 0 | Tetracaine 0.2% | - 0 ± 0 |
Procaine 1.0% | -34±10 | Tetracaine 0.3% | - 0± 0 |
Si on compare cette technique avec les autres utilisées pour ľévaluation ďun blocage, on constate qu’elle nous permet une évaluation améliorée, standardisée et fiable des propriétés analgésiques des agents anesthésiques locaux.
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References
Albért, J. &Löfström, B. Bilateral ulnar nerve blocks for the evaluation of local anesthetic agents. Acta Anesthesiol. Scand.5: 99 (1961).
Albért, J. &Löfström, B. Bilateral nerve blocks for the evaluation of local anesthetic agents. Acta Anesthesiol. Scand.9: 1 (1965).
Löfström, B. The blocking effects of some local anesthetics commonly used in clinical practice. International Encyclopedia of Pharmacology and Therapeutics, sect. 8,1: 167 (1971).
Löfström, B. Ulnar nerve blockade for the evaluation of local anesthetic agents. Br. J. Anaesth.47: 297 (1975).
Camougis, G. &Takman, B. Nerve and nerve-muscle preparations (as applied to local anesthetics). Methods Pharmacol.1: 1(1971).
Truant, A. Studies on the pharmacology of meprylcaine (oracaine), a local anesthetic. Arch. Int. Pharmacodyn. Ther.115: 483 (1958).
Truant, A. &Wiedling, S. A contribution to the pharmacological and toxicological evaluation of a new local anesthetic di-N-methyl-pipecolyl-2, 6-xylidide. Acta Chir. Scand.116: 351 (1958–59).
Aström, A. &Persson, N. Some pharmacological properties of o-methyl-d-propyl-amino propionanilide, a new local anesthetic. Br. J. Pharmacol.16: 32 (1961).
Alberg, G. Toxicologic and local anesthetic effects of optically active isomers of two local anesthetic compounds. Acta Pharmacol. Toxicol.31: 273 (1972).
Adams, H.el al. Local anesthetic activity and acute toxicity of (±)-2-(N-ethyl-propylamin)-2′,6′-butyroxylidide, a new long-acting agent. J. Pharm. Sci.61: 1829 (1972).
Schackell, L. Tests of local anesthetics by sciatic nerve block in the intact guinea pig. Anesth. Analg.14: 20 (1935).
Luduena, F. &Hoppe, J. 2-phenyl-4-thiazolidone derivatives as local anesthetics. J. Am. Pharm. Assoc.40: 132 (1951).
Fink, B. Neurokinetics of lidocaine in the infraorbital nerve of the ratin vivo. Anesthesiology42: 731 (1975).
Bonica, J. Clinical investigation of local anesthetics. Anesthesiology18: 110 (1957).
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Ready, L.B., Fink, B.R. Experimental evaluation of local anaesthetic solutions using rat infraorbital nerve block. Canad. Anaesth. Soc. J. 27, 58–61 (1980). https://doi.org/10.1007/BF03006850
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DOI: https://doi.org/10.1007/BF03006850