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Modulation of early steps in insulin action in the liver and muscle of epinephrine treated rats

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Abstract

Epinephrine is known to produce insulin resistance, but the exact molecular mechanism involved is unknown. In the present study we have examined the levels and phosphorylation state of the insulin receptor and of insulin receptor substrate 1 (IRS-1), as well as the association between IRS-1 and phosphatidylinositol 3-kinase (PI 3-kinase) in the liver and muscle of rats treated with epinephrine. The results demonstrate a decrease in insulin-stimulated receptor and IRS-1 phosphorylation levels which was accompanied by a reduction in the association of IRS-1 with PI 3-kinasein vivo in liver and muscle of epinephrine treated rats. These data suggest that molecular post-receptor defects may explain some aspects of the insulin resistance induced by catecholamines.

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References

  • Araki, E., Lipes, M.A., Patti, M.A., Bruning, J.C.,Haag, B., Johnson, R.S. & Kahn, C.R. (1994).Nature,372, 186–190.

    Article  PubMed  CAS  Google Scholar 

  • Backer, J.M., Myers, M.G., Jr., Shoelson, S.E., Chin, DJ., Sun, X.J., Miralpeix, M., Hu, P., Margolis, B., Skolnik, E.Y., Schlessinger, J. & White, M.F. (1992).EM BO J.,11, 3469–3479.

    CAS  Google Scholar 

  • Bonen, A., Megeney, L.A., McCarthy, S.C, McDermott, J.C. & Tan, M.H. (1992).Biochem. Biophys. Res. Commun.,187, 685–691.

    Article  PubMed  CAS  Google Scholar 

  • Bradford, M.M. (1976).Anal. Biochem.,72, 248–254.

    Article  PubMed  CAS  Google Scholar 

  • Cheatham, B., Vlahos, C.J., Cheatham, L., Wang, L., Blenis, J. & Kahn, C.R. (1994).Mol. Cell, Biol.,14, 4902–4911.

    CAS  Google Scholar 

  • Chiasson, J.L., Shikama, H., Chu, D.T.W. & Exton, J.H. (1981).J. Clin. Invest.,68, 706–713.

    Article  PubMed  CAS  Google Scholar 

  • Ebina, Y., Araki, E., Taira, M., Shimada, F., Mori, M., Craik, C.S., Siddle, K., Pierce, S.B., Roth, R.A. & Rutter, W.J. (1987).Proc. Natl. Acad. Sci. USA,84, 704–708.

    Article  PubMed  CAS  Google Scholar 

  • Folli, F., Saad, M.J.A., Backer, J.M. & Kahn, C.R. (1992).J. Biol. Cham.,267, 22171–22177.

    CAS  Google Scholar 

  • Folli, F., Saad, M.J.A., Backer, J.M. & Kahn, C.R. (1993).J. Clin. Invest.,92, 1787–1794.

    Article  PubMed  CAS  Google Scholar 

  • Giorgetti, S., Balloti, R., Kowalski-Chauvel, A., Tartare, S. & Van Obberghen, E. (1993).J. Biol. Chem.,268, 7358–7364.

    PubMed  CAS  Google Scholar 

  • Hadari, Y.R., Tzahar, E., Nadiv, O., Rothenberg, P., Roberts, C.T., LeRoith, D., Yarden, Y. & Ziek, Y. (1992).J. Biol. Chem.,267, 17483–17486.

    PubMed  CAS  Google Scholar 

  • Joost, H.G., Weber, T.M., Cushman, S.W. & Simpson, I.A. (1986).J. Biol. Chem.,261, 10033–10036.

    PubMed  CAS  Google Scholar 

  • Kanai, F., Ito, K., Todara, M., Hayashi, H., Kamahara, S., Ishi, K., Okada, T., Hazeki, O., Ui, M. & Ebina, Y. (1993).Biochem. Biophys. Res. Commun.,197, 762–768.

    Article  Google Scholar 

  • Kasuga, M., Karlsson, R.A. & Kahn, C.R. (1982).Science,215, 185–187.

    Article  PubMed  CAS  Google Scholar 

  • Kelly, K.L. & Ruderman, N.B. (1993).J. Biol. Chem.,268, 4391–4398.

    PubMed  CAS  Google Scholar 

  • Kirsch, D., Kemmler, W. & Haring, H.U. (1983).Biochem. Biophys. Res. Commun.,115, 389–405.

    Article  Google Scholar 

  • Kuhne, M.R., Pawson, T., Lienhard, G.E. & Feng, G.H. (1993).J. Biol. Chem.,268, 11479–11481.

    PubMed  CAS  Google Scholar 

  • Kuroda, M., Honnor, R.C., Cushman, S.W., Londos, C. & Simpson, I.A. (1987).J. Biol. Chem.,262, 245–353.

    PubMed  CAS  Google Scholar 

  • Laemmli, U.K. (1970).Nature,227, 680–685.

    Article  PubMed  CAS  Google Scholar 

  • Lavan, B.E., Kuhne, M.R., Garner, C.W., Anderson, D., Reedijk, M., Pawson, T. & Lienhard, G.E. (1992).J. Biol. Chem.,267, 11631–11636.

    PubMed  CAS  Google Scholar 

  • Maegawa, H., Olefsky, J.M., Thies, S., Boyd, D., Ullrich, A. & McClain, D.A. (1988).J. Biol. Chem.,263, 12629–12637.

    PubMed  CAS  Google Scholar 

  • Pessin, J.E., Gitomer, W., Oka, Y., Oppenheimer, C.L. & Czech, M.P. (1983).J. Biol. Chem.,258, 7386–7394.

    PubMed  CAS  Google Scholar 

  • Quon, J.M., Butte, A.J., Zarnowski, M.J., Sesti, G., Cushman, S.W. & Taylor, S.I. (1994).J. Biol. Chem.,269, 27920–27924.

    PubMed  CAS  Google Scholar 

  • Rosen, O. (1987).Science,237, 1452–1458.

    Article  PubMed  CAS  Google Scholar 

  • Roth, R.A. & Beaudoin, J. (1987).Diabetes,36, 123–126.

    Article  PubMed  CAS  Google Scholar 

  • Rothenberg, P.L., Lane, W.S., Karasik, A., Backer, J., White, M.F. & Kahn, C.R. (1991).J. Biol. Chem.,266, 8302–8311.

    PubMed  CAS  Google Scholar 

  • Saad, M.J.A., Araki, E., Miralpeix, M., Rothenberg, P.L., White, M.F. & Kahn, C.R. (1992).J. Clin. Invest.,90, 1839–1849.

    Article  PubMed  CAS  Google Scholar 

  • Saad, M.J.A., Folli, F., Kahn, J.A. & Kahn, C.R. (1993).J. Clin. Invest.,92, 2065–2072.

    Article  PubMed  CAS  Google Scholar 

  • Skolnik, E.Y., Lee, C.H., Batzer, A., Vicentini, L.M., Zhou, M., Myers, M.G., Backer, J.M., Ulrich, A., White, M.F. & Schlessinger, J. (1993).EMBO J.,12, 1926–1936.

    Google Scholar 

  • Stadmauer, L. & Rosen, O.M. (1986).J. Biol. Chem.,261, 3402–3407.

    Google Scholar 

  • Sun, X.J., Rothenberg, P.L., Kahn, CR., Backer, J.M., Araki, E., Wilden, P., Cahill, D.A., Goldstein, B.J. & White, M.F. (1991).Nature,352, 73–77.

    Article  PubMed  CAS  Google Scholar 

  • Tamemoto, H., Kadowaki, T., Tobe, K., Yagi, T., Sakura, H., Hayakawa, T., Terauchi, Y., Ueki, K., Kaburagi, Y., Satoh, S., Sekihara, H., Yoshioka, S., Horlkoshi, H., Furuta, Y., Ikawa, Y., Kasuga, M., Yazaki, Y. & Aizawa, S. (1994).Nature,372, 182–186.

    Article  PubMed  CAS  Google Scholar 

  • Towbin, H., Staehlin, J. & Gordon, J. (1979).Proc. Natl. Acad. Sci. USA,76, 4350–4354.

    Article  PubMed  CAS  Google Scholar 

  • Vannucci, S.J., Nishimura, H., Satoh, S., Cushman, S.W., Holman G.D. & Simpson, I.A. (1992).Biochem. J.,288, 325–330.

    PubMed  CAS  Google Scholar 

  • Wallberg-Henriksson, H. (1987).Acta Physiol. Scand. (Suppl.)564, 1–80.

    CAS  Google Scholar 

  • Wang, L., Keegan, A.D., Li, W., Lienhard, G.E., Pacini, S., Gutkind, W.E., Myers, M.G., Sun, X.J., White, M.F., Aaronson, S.A., Paul, W.E. & Pierce, J.H. (1993).Proc. Natl. Acad. Sci. USA,90, 4032–4036.

    Article  PubMed  CAS  Google Scholar 

  • Weber, T.M., Joost, H.G., Kuroda, M., Cushman, S.W. & Simpson, I.A. (1991).Cell Signal.,3, 51–58.

    Article  PubMed  CAS  Google Scholar 

  • White, M.F., Maron, R. & Kahn, C.R. (1985).Nature,318, 183–186.

    Article  PubMed  CAS  Google Scholar 

  • Wilson, G.F. & Kaczmarek, L.K. (1993).Nature,366, 433–438.

    Article  PubMed  CAS  Google Scholar 

  • Yonezawa, K., Ueda, H., Hara, K., Nishida, K., Ando, A., Chavanieu, A., Matsuba, H., Shii, K., Yokono, K., Fukui, Y., Calas, B., Grisorescu, F., Dhand, R., Gout, I., Otsu, M., Waterfield, M.D. & Kasuga, M. (1992).J. Biol. Chem.,267, 25958–25966.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Internal Medicine, FCM, UNICAMP, 13081-970, Campinas, SP, Brazil

    Mario J. A. Saad, Luiz G. C. Hartmann, Daniela S. de Carvalho, Cesar A. O. Galoro, Sigisfredo L. Brenelli & Carla R. O. Carvalho

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  1. Mario J. A. Saad
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  2. Luiz G. C. Hartmann
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  3. Daniela S. de Carvalho
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  4. Cesar A. O. Galoro
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  5. Sigisfredo L. Brenelli
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  6. Carla R. O. Carvalho
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Saad, M.J.A., Hartmann, L.G.C., de Carvalho, D.S. et al. Modulation of early steps in insulin action in the liver and muscle of epinephrine treated rats. Endocr 3, 755–759 (1995). https://doi.org/10.1007/BF03000209

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