Abstract
The estradiol effects on breast cancer cell lines including estrogen receptors (ER) positive and negative were studied with flow cytometry analysis, scanning and transmission electron microscopy (SEM and TEM), immunogold and immunofluorescence staining techniques. The results showed that estradiol markedly stimulated the division and proliferation of the ER(+) MCF-7 cells at 10 nM, but had no marked effect on the cell cycle of the ER(−) H466B cells at the same concentration, and that tamoxifen inhibited the stimulation of estradiol on MCF-7 cells. Estradiol obviously influenced the ultrastructure of MCF-7 cells. Immunocytochemical localization of epidermal growth factor receptor (ECFR) on the MCF-7 cell membrane surface indicated that one of the mechanisms involving the growth of MCF-7 breast cancer cell and the stimulating effect on MCF-7 cells growth by estradiol is autocrine secretion.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Vignon F, Terqui M, et al. Effect of plasma estrogen sulfate in mammary cancer cells. Endocrinology 1980; 106:1079.
Patrice V, et al. Effect of estradiol on the ultrastructure of the MCF-7 human breast cancer cells in culture. Cancer Research 1982; 42: 667.
Staros JV, et al. Molecular mechanisms of transmembrane signalling. Cohen Houslay 1985; 253.
Sporn MB, et al. Autocrine secretion and malignant transformation of cells. N Engl J Med 1980; 303:878.
Kasid A, et al. Estrogen and oncogene mediated growth regulation of human breast cancer cells. J Steroid Biochem 1987; 27:465
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Zhang, X., Chen, D., Lu, Y. et al. The effects of estradiol on breast cancer cell lines. Chin J Cancer Res 2, 31–36 (1990). https://doi.org/10.1007/BF02997560
Issue Date:
DOI: https://doi.org/10.1007/BF02997560