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In vitro exchangeable erythrocytic zinc

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Abstract

Exchangeable erythrocytic zinc is measured by65Zn uptake in and release from erythrocytes under standarized and near, physiological conditions: 7.6 μM zinc and 580 μM albumin in the medium. The intracellular exchangeable erythrocytic zinc pool in healthy volunteers amounts to 5 μmol zinc/L packed cells. The half-time of the exchange is 7 h, its activation energy 84 kJ/mol. The effects of the variation in temperature and the concentrations of albumin, as well as the effects of some zinc carriers, cell transport inhibitors, and stress hormones on the65Zn uptake are measured.

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References

  1. B. S. W. Smith and H. Wright,Biochim. Biophys. Acta 307, 590 (1973).

    Article  PubMed  CAS  Google Scholar 

  2. K. E. C. De Haan, C. J. De Groot, C. J. A. Van den Hamer, and H. Boxma,Clin. Chim. Acta 170, 111 (1987).

    Article  PubMed  Google Scholar 

  3. Analytical Methods for Atomic Absorption Spectrophotometry, Perkin-Elmer, Norwalk, CT (1976).

  4. R. Whittam,Transport and diffusion in red blood cells, Edward Arnold, London, 1964, pp. 83, 126.

    Google Scholar 

  5. P. L. McWilliams, R. P. Agarwal, and R. I. Henkin,Clin. Res. 31, 243A (1983).

    Google Scholar 

  6. H. Ohno, R. Doi, K. Yamamura, K. Yamashita, S. Iizuka, and H. Taniguchi,Blut 50, 113 (1985).

    Article  PubMed  CAS  Google Scholar 

  7. W. C. Kruckeberg and G. J. Brewer,Med. Biol. 56, 5 (1978).

    PubMed  CAS  Google Scholar 

  8. G.-J. van den Berg and C. J. A. van den Hamer,J. Inorg. Biochem. 24, 289 (1985).

    Article  PubMed  Google Scholar 

  9. A. W. Wolkoff,Hepatology 7, 777 (1987).

    Article  PubMed  CAS  Google Scholar 

  10. W. Fuchswans and H. Springer-Lederer,Recent advances in membrane and metabolic research, E. Gerlach, K. Moser, E. Deitsch, and W. Wilmanns, eds., Thieme-Verlag, Stuttgart, FRG, 1973, pp. 121, 122.

    Google Scholar 

  11. R. K. Berry, M. C. Bell, and P. L. Wright,J. Nutr. 88, 284 (1966).

    PubMed  CAS  Google Scholar 

  12. J. K. Chesters and M. Will,Br. J. Nutr. 38, 297 (1978).

    Article  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Radiochemistry, Interfaculty Reactor Institute, Delft University of Technology, Mekelweg 15, 2629 JB, Delft, the Netherlands

    Jacobus P. Van Wouwe, Jeroen J. M. De Goeij & Cornelis J. A. Van den Hamer

  2. Department of Paediatrics, State University at Leiden, PO Box 9600, 2300 RC, Leiden, the Netherlands

    Jacobus P. Van Wouwe & Marcel Veldhuizen

Authors
  1. Jacobus P. Van Wouwe
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  2. Marcel Veldhuizen
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  3. Jeroen J. M. De Goeij
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  4. Cornelis J. A. Van den Hamer
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Van Wouwe, J.P., Veldhuizen, M., De Goeij, J.J.M. et al. In vitro exchangeable erythrocytic zinc. Biol Trace Elem Res 25, 57–69 (1990). https://doi.org/10.1007/BF02990265

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  • DOI: https://doi.org/10.1007/BF02990265

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