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Analysis of three-dimensional cell imaging obtained with optical microscopy techniques based on defocusing

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Abstract

The properties of an optical microscope are analyzed and analytically evaluated with a simple and effective model in order to understand the true meaning, limitations, and real capabilities of a defocusing technique.

Major emphasis is given to the applications related to microscopic objects of biological interest using fluorescence and absorption light microscopy. A procedure for three-dimensional viewing is analyzed and discussed.

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Authors and Affiliations

  1. Department of Biophysical and Electronic Engineering, Via All’Opera Pia 11A, Genova, Italy

    B. Bianco

  2. Interdepartmental Center for Biomedical and Biophysical Technologies, Viale Benedetto XV 2, Genova, Italy

    B. Bianco & A. Diaspro

  3. University of Genoa, Italy

    B. Bianco & A. Diaspro

Authors
  1. B. Bianco
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  2. A. Diaspro
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Bianco, B., Diaspro, A. Analysis of three-dimensional cell imaging obtained with optical microscopy techniques based on defocusing. Cell Biophysics 15, 189–199 (1989). https://doi.org/10.1007/BF02989683

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  • DOI: https://doi.org/10.1007/BF02989683

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