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Research Articles: Special Issue “Impact Studies on Cloned Poplars”

Part I: Suitability and use of poplars as bioindicators — A new concept (ESPR 1/1997) Part II: Ozone and heavy metals from automobile catalytic converters (ESPR 3/1997) Part III: Theoretical aspects (ESPR 4/1997)

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Abstract

Practical considerations, characteristic physiological features, as well as the availability of hybrids with different resistances to air pollutants play an important role in the decision to use poplar cuttings as experimental plants for impact studies.

This paper demonstrates the effect of the hybridization of two poplar clones on the resistance to different air pollutants and, additionally, presents a new concept of bioindication involving these two clones with different degrees of resistance to SO2 and O3.

This concept, which is based on the results of fumigation experiments conducted in open-top chambers, uses six-week-old vital poplar cuttings as bioindicators. Both the anabolic defense reactions of younger leaves and the catabolic stress reactions of older leaves are examined. The measurement results obtained from cv. Loenen and cv. Rochester clones which differ in their sensitivities to individual pollutants, are examined to determine the specific relationships. The results can be verified by measuring the premature leaf losses of a third clone, cv. Unal, which reacts to both SO2 and O3 with highly pronounced leaf loss.

To prevent false interpretations, it is necessary to standardize the plant material as far as possible by choosing clones of a defined age and number of leaves, and by selecting reproducible cultivation and exposure conditions. The impact criteria are methodologically simple and therefore suitable for routine examinations.

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References

  • Arndt, U. (1971): Konzentrationsänderungen bei Blattfarbstoffen unter dem Einfluß von Luftverunreinigungen. Ein Diskussionsbeitrag zur Pigmentanalyse. Environ. Pollut.2, 37–48

    Article  CAS  Google Scholar 

  • Arndt, U. (1974): The Kautsky-effect: A method for the investigation of air pollutants in chloroplasts. Environ. Pollut.6, 181–194

    Article  CAS  Google Scholar 

  • Arndt, U. (1992): Einführung in die Bioindikation. Hohenheimer Umwelttagung24, 13–18

    CAS  Google Scholar 

  • Ballach, H.-J. (1990): Application of a new HPLC-system to the analysis of several chlorophylls and carotenoids from pigment biosynthesis and degradation of higher plants. Acta Biol. Benrodis2, 191–208

    Google Scholar 

  • Ballach, H.-J.;J Mooi;R. Wittig (1992a): Premature aging inPopulus nigra L. after exposure to air pollution. Angew. Bot.66, 14–20

    CAS  Google Scholar 

  • Ballach, H.-J.;S. Oppenheimer;J. Mooi (1992b): Reactions of cloned poplars to air pollution: Premature leaf loss and investigations of the nitrogen metabolism. Z. Naturforsch.47c, 109–119

    Google Scholar 

  • Ballach, H.-J. (1995): Untersuchungen zum Immissionsstreß klonierter Pappeln. Verh. Ges. Ökol.24, 249–254

    Google Scholar 

  • Ballach, H.-J.;C. Niederée;R. Wittig;E. J. Woltering (1995): Reactions of cloned poplars to air pollution — ozone-induced increase of stress ethylene and possible antisenescence strategies. Environ. Sci. & Pollut. Res.2, 201–206

    CAS  Google Scholar 

  • Beckerson, D.W.;G. Hofstra (1979): Response of leaf diffusive resistance of radish, cucumber and soybean to O3 and SO2 singly or in combination. Atmos. Environ.13, 1263–1268

    Article  CAS  Google Scholar 

  • Beutler, H.O. (1978): Enzymatische Bestimmung von Stärke in Lebensmitteln mit Hilfe der Hexokinase-Methode. Stärke30, 309–312

    Article  CAS  Google Scholar 

  • Black, V.J. andM.H. Unsworth (1979): Effects of low concentrations of sulphur dioxide on net photosynthesis and dark respiration ofVicia faba. J. Exp. Bot.30, 473–483

    Article  CAS  Google Scholar 

  • Bressan, R.A.;L. Le Cureux;L.G Wilson;P. Filner (1979): Emission of ethylene and ethane by leaf tissue exposed to injurious concentrations of sulphur dioxide or bisulphite ions. Plant Physiol.63, 924–930

    CAS  Google Scholar 

  • Bücker, J. (1991): Immissionsbedingte Störungen im Kohlenhydrathaushalt junger Pappeln und Fichten. Westarp Wiss., Essen

    Google Scholar 

  • Bücker, J.;H.-J. Ballach (1992): Alterations in carbohydrate levels in leaves ofPopulus due to air pollution. Physiol. Plant.86, 512–517

    Article  Google Scholar 

  • Bücker, J. (1995): Unterschiedliche Pappelvarietäten als Grundlage zur Ausarbeitung einer biochemischen Indikation pflanzengefährdender O3- und SO2-Konzentrationen. Verh. Ges. Ökol.24, 255–258

    Google Scholar 

  • Butler, L.K.;T.W. Tibbitts (1979): Stomatal mechanisms determining genetic resistance to ozone inPhaseolus vulgaris L. J. Am. Soc. Hort. Sci104, 213–216

    Google Scholar 

  • Bylinska, E.;J. Sarosiek (1980): Die unterschiedliche Empfindlichkeit der Pappeln auf die chemische Luftverseuchung in der urban-industriellen Agglomeration von Wroclac. In:Spanley, J. (Ed.), Proc. III rd Int. Conf. Bioindicators Deteriosationis Regionis. Libice, Czecheslovakia. Academia Praha, 91–97

    Google Scholar 

  • Ceulemans, R.J.;I. Impens;V. Steenackers (1988): Genetic variation in aspects of leaf growth ofPopulus clones, using the leaf plastochron index. Can. J. For. Res.18, 1069–1077

    Article  Google Scholar 

  • Ceulemans, R.J. (1990): Genetic variation in functional and structural productivity determinants in poplar. Thesis Publishers, Amsterdam, 25–41

    Google Scholar 

  • Darrall, N.M. (1989): The effects of air pollutants on physiological processes in plants. Plant Cell Environ.12, 1–30

    Article  CAS  Google Scholar 

  • Djingova, R.;I. Kuleff (1993): Monitoring of heavy metal pollution byTaraxacum officinale. In:B. Marken (Ed.): Plants as Biomonitors. VCH, New York, 435–460

    Google Scholar 

  • Dochinger L.S.;K.F. Jensen (1975): Effects of chronic and acute exposure to sulphur dioxide on the growth of hybrid poplar cuttings. Environ. Pollut.9, 219–229

    Article  CAS  Google Scholar 

  • Erickson, R.O.;F.J. Michelini (1957): The plastochron index. Am. J. Bot.44, 297–305

    Article  Google Scholar 

  • Freer-Smith, P.H. (1984): The responses of six broad-leaved trees during long-term exposure to SO2 and NO2. New Phytol.97, 49–61

    Article  CAS  Google Scholar 

  • Greszta, J.;M. Wierzbicki (1980): Die Widerstandsfähigkeit einzelner Abarten der Pappel gegen Luftverunreinigungen durch die Immission. In:Spanley, J. (Ed.): Proc. IIIrd Conf. Bioindicatores Deteriorisationes Regionis. Liblice, Czechoslovakia, Academia Praha, 83–40

    Google Scholar 

  • Heath, R.L. (1978): The reaction stoichiometry between ozone and unsaturated fatty acids in an aqueous environment. Chem. Phys. Lipids22, 25–37

    Article  CAS  Google Scholar 

  • Jäger, H.J. (1975): Wirkung von SC2-Begasung auf die Aktivität von Enzymen des Aminosäurestoffwechsels und den Gehalt freier Aminosäuren in unterschiedlich resistenten Pflanzen. Z. Pflanzenkrankh. Pflanzenschutz82, 139–148

    Google Scholar 

  • Jäger, H.J.;H. Klein (1977): Biochemical and physiological detection of sulphur dioxide injury to pea plants (Pisum sativum). J. Air. Pollut. Control Assoc.27, 464–466

    Google Scholar 

  • Jensen, K.F.;L.S. Dochinger (1974): Responses of hybrid poplar cuttings to chronic and acute levels of ozone. Environ. Pollut.6, 289–295

    Article  CAS  Google Scholar 

  • Keller, T. (1974): The use of peroxidase activity for monitoring and mapping air pollution areas. Eur. J. For. Path.4, 11–19

    Article  CAS  Google Scholar 

  • Keller, T. (1988): Growth and premature leaf fall in American Aspen as bioindicators for ozone. Environ. Pollut.52, 183–192

    Article  CAS  Google Scholar 

  • Kimmerer, T.W.;T.T. Kozlowski (1981): Stomatal conductance and sulfur uptake of five clones ofPopulus tremuloides exposed to sulfur dioxide. Plant. Physiol.67, 990–995

    CAS  Google Scholar 

  • Koziol, M.J. (1984): Interactions of gaseous pollutants with carbohydrate metabolism. In:Koziol, M.J. andF.R. Whatley (Eds.): Gaseous air pollutants and plant metabolism. Butterworths, London, 251–273

    Google Scholar 

  • Koziol, M.J.;F.R. Whatley;J.D. Shelvey (1988): An integrated view of the effects of gaseous air pollutants on plant carbohydrate metabolism. In:Schulte-Hostede, S., N.M. Darrall, L.W. Blank andA.R. Wellburn (Eds.): Air pollution and plant metabolism. Elsevier, London, 148–168

    Google Scholar 

  • Langebartels, C.;K. Kerner;S. Leonardi;M. Schraudner;M. Trost;W. Heller;H. Sandermann (1991): Biochemical plant responses to ozone. I. Differential induction of polyamine and ethylene biosynthesis in tobacco. Plant Physiol.95, 882–889

    CAS  Google Scholar 

  • Larcher, W. (1969): The effect of environmental and physiological variables on the carbon dioxide exchange of trees. Photosynthetica3, 167–198

    CAS  Google Scholar 

  • Lee, E.H.;J.H. Bennett (1982): Superoxide dismutase. A possible protective enzyme against ozone injury in snap beans (Phaseolus vulgaris L.). Plant Physiol.69, 1555–1459

    Article  Google Scholar 

  • Levitt, J. (1972): Responses of plants to environmental stresses. Academic Press, New York

    Google Scholar 

  • Mansfield, T.A.;P.H. Freer-Smith (1984): The role of stomata in resistance mechanisms. In:Koziol, M.L. andF.R. Whatley (Eds.): Gaseous pollutants and plant metabolism. Butterworth, London, 131–146

    Google Scholar 

  • Mehlhorn, H.;G. Seufert;A. Schmidt;K.J. Kunert (1986): Effect of SO2 and O3 on production of antioxidants in conifers. Plant Physiol.38, 605–609

    Google Scholar 

  • Meijer, E.M.J.;J. Mooi (1987): Technische beschrijving/handleiding von de OTC-begassings installatie te Apeldoorn. Research Institute for Plant Protection, Wageningen, The Netherlands,R 353, 1–76

    Google Scholar 

  • Mooi, J. (1981): Influence of ozone and sulphur dioxide on defoliation and growth of poplars. Mitt. Forstl. Bundesversuchsanstalt (Wien)137, 47–51

    CAS  Google Scholar 

  • Mooi, J. (1983): Responses of some poplar species to mixtures of SO2, NO2 and O3. Aquilo Ser. Bot.19, 189–196

    CAS  Google Scholar 

  • Mooi, J. (1984): Responses of some poplar species to mixtures of SO2, NO2 and O3. Aquilo Ser. Bot.19, 189–196

    Google Scholar 

  • Mooi, J. (1989): Effecten van rookgas afkomstig van een kolengestookte installatie op planten. IPO-Report, R 380

  • Noble, R. D.;K.F. Jensen (1980): Effects of sulphur dioxide and ozone on growth of hybrid poplar leaves. Am. J. Bot.67, 1005–1009

    Article  CAS  Google Scholar 

  • Pallardy, S.G.;T.T. Kozlowski (1981): Water relations ofPopulus clones. Ecology62, 159–169

    Article  Google Scholar 

  • Pande, P.C. (1985): An examination of the sensitivity of five barley cultivars to SO2 pollution. Environ. Pollut.37, 27–41

    Article  CAS  Google Scholar 

  • Patton, R.L.;M.O. Garroway (1986): Ozone-induced necrosis and increased peroxidase activity in hybrid poplar (Populus spec.) leaves. Environ. Exp. Bot.26, 137–141

    Article  CAS  Google Scholar 

  • Peiser, G.D.;S.F. Yang (1979): Ethylene and ethane production from sulfur dioxide-injured plants. Plant Physiol.63, 142–145

    CAS  Google Scholar 

  • Pilgram, C.;U. Arndt (1987): Wirkungsbezogene Erfassung der Immissionsbelastung durch den Einsatz von Bioindikatoren (aktives Monitoring von Indikatorpflanzen). PEF-Berichte12, 101–111

    Google Scholar 

  • Raab, M.M.;L.H. Weinstein (1990): Polyamine and ethylene metabolism inTriticum aestivum. In:Flores, H.E., R.N. Arteca, J.C. Shannon (Eds.): Polyamines and ethylene: biochemistry, physiology and interactions. American Society of Plant Physiologists, New York, 408–410

    Google Scholar 

  • Rabe, E.;K.H. Kreeb (1980): Bioindication by chlorophyll destruction in plant leaves. Oikos34, 163–167

    Article  CAS  Google Scholar 

  • Schmieden, U. (1991): Untersuchungen antioxidativer Schutzsysteme im Hinblick auf Kausalanalyse und Bioindikation “Neuartiger Waldschäden”. Dissertation Johannes Gutenberg-Universität Mainz

  • Schreiber, U.;W. Vidaver;V.C. Runeckles;P. Rosen (1978): Chlorophyll fluorescence assay for ozone injury in intact plants. Plant Physiol.61, 80–84

    CAS  Google Scholar 

  • Siefermann-Harms, D. (1992): The yellowing of spruce in polluted atmospheres. Photosynthetica27, 323–341

    CAS  Google Scholar 

  • Stettler, R.F.;R.J. Ceulemans (1993): Clonal material as a focus for genetic and physiological research in forest trees. In:Ahuja, M.R. andW.J. Libby (Eds.): Clonal Forestry I: Genetics and Biotechnology. Springer-Verlag, Berlin, 68–86

    Google Scholar 

  • Stettler, R.F;H.D. Bradshaw, Jr. (1994): The choice of genetic material for mechanistic studies of adaption in forest trees. Tree Physiol.14, 781–796

    Google Scholar 

  • Stitt, M. (1991): Fructose-2,6-biphosphate as a regulatory molecule in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol.41, 153–185

    Article  Google Scholar 

  • Taylor, G.E., Jr. (1978): Plant and leaf resistance to gaseous air pollution stress. New Phytol.80, 523–534

    Article  CAS  Google Scholar 

  • Tietz, S.;A. Wild (1991): Investigations on the phosphoenolpyruvate carboxylase activity of spruce needles relative to the occurrence of novel forest decline. J. Plant Physiol.137, 327–331

    CAS  Google Scholar 

  • Tingey, D.T.;R.G. Wilhour;C. Standley (1976): The effect of chronic ozone exposure on the metabolite content of Ponderosa pine seedlings. For. Sci.22, 234–241

    CAS  Google Scholar 

  • Wagner, G. (1984): Schwermetallanalysen in Blättern von Pyramidenpappeln zur Überwachung der Umweltbelastung. Fortschr. atomspektrometr. Spurenanalytik1, 609–620

    CAS  Google Scholar 

  • Wagner, G. (1993): Large-scale screening of heavy metal burdens in higher plants. In:B. Markert (ed.): Plants as Biomonitors. VCH, New York, 425–434

    Google Scholar 

  • Weigel, H.-J.;H.-J. Jäger (1985): Physiologische und biochemische Verfahren zum Nachweis von Schadstoffwirkungen. Staub Reinhalt. Luft45, 269–271

    CAS  Google Scholar 

  • Wild, A.;V. Schmitt (1990): Biochemische und feinstrukturelle Untersuchungen an Blattorganen von Buchen und Fichten im Rahmen der Projekte “Begasungsversuch Kettwig” und “Open-Top-Kammer-Versuch Eggegebirge” der Landesanstalt für Immissionsschutz (LIS). Forschungsbericht zum Forschungsvorhaben des Landes Nordrhein-Westfalen “Luftverunreinigungen und Waldschäden”18, 26. 1–26.18

    Google Scholar 

  • Wild, A.;V. Schmitt;S. Tietz-Siemer (1994): Biochemische Schadindikation der Neuartigen Waldschäden bei Fichten — PEPC-Kataster Nordrhein-Westfalen. Forschungsberichte zum Forschungsprogramm des Landes Nordrhein-Westfalen “Luftverunreinigungen und Waldschäden”36, 73–83

    Google Scholar 

  • Wolfenden, J.;D.C. Robinson;J.N. Cape;I.S. Paterson;B.J. Francis;H. Mehlhorn;A.R. Wellburn (1988): Use of carotenoid ratios, ethylene emissions and buffer capacities for the early diagnosis of forest decline. New Phytol.109, 85–95

    Article  CAS  Google Scholar 

  • Wolfenden, J.;P.A. Wookey;P.W. Lucas;T.A. Mansfield (1992): Action of pollutants individually and in combination. In:Barker, J.R. andD.T. Tingey (Eds.): Air pollutant effects on biodiversity. Van Nostrand Reinhold, New York, 72–92

    Google Scholar 

  • Zelawski, W. (1973): Gas exchange and water relations. In:Bialobok, S. (Ed.): The poplars —Populus L. National Technical Information Service12, 149–165, United States Department of Commerce, Springfield, Virginia, USA

    Google Scholar 

  • Ziegler, R.;K. Egle (1965): Zur quantitativen Analyse der Chloroplastenpigmente. I. Kritische Überprüfung der spektrophotometrischen Chlorophyll-Bestimmung. Beitr. Biol Pflanzen41, 11–37

    CAS  Google Scholar 

  • Zwoch, I.;U. Knorre;H. Schaub (1989): Influence of SO2 and O3, singly or in combination, on ethylene synthesis in sunflower. Environ. Exp. Bot.30, 193–205

    Article  Google Scholar 

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  1. Botanical Institute of Johann Wolfgang Goethe-University, Siesmayerstrasse 70, D-60323, Frankfurt/Main, Germany

    Hans-Joachim Ballach

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Ballach, HJ. Research Articles: Special Issue “Impact Studies on Cloned Poplars”. Environ. Sci. & Pollut. Res. 4, 37–45 (1997). https://doi.org/10.1007/BF02986263

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