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An enzyme immunoassay for detection of Japanese encephalitis virus-induced chemotactic cytokine

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Abstract

Japanese encephalitis virus (JEV) induces human peripheral blood monocytes to secrete a chemotactic cytokine [human macrophage-derived factor (hMDF)] which causes chemotaxis of neutrophils. The only known assay for hMDF cannot quantify its level in samples, so an enzyme immunoassay has been standardized for detection of hMDF and hMDF-specific antibodies in test samples. The reported enzyme linked immunosorbent assay (ELISA) was found to be sensitive (89%), specific (91%), accurate (92.2%) and reproducible and was able to detect a minimum concentration of 23 ng hMDF/ml in test samples. The chemotactic factor could be detected in JEV inoculated mouse sera and JEV infected culture fluids. Significant finding of the test was the detection of hMDF in sera of human cases of JE.

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Abbreviations

ELISA:

Enzyme linked immunosorbent assay

JEV:

Japanese encephalitis virus

hMDF:

human macrophage-derived factor

HBSS:

Hank’s balanced salt solution

CSF:

cerebrospinal fluid

MEM:

minimum essential medium

FCS:

foetal calf serum

PBS:

phosphate buffered saline

NS:

normal saline

As:

antisera

HRP:

horse raddish peroxiidase

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Authors and Affiliations

  1. Postgraduate Department of Microbiology, KG’s Medical College, 226 003, Lucknow, India

    Aditi Singh, Rajesh Kulshreshtha & Asha Mathur

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  1. Aditi Singh
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  2. Rajesh Kulshreshtha
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  3. Asha Mathur
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Correspondence to Asha Mathur.

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Singh, A., Kulshreshtha, R. & Mathur, A. An enzyme immunoassay for detection of Japanese encephalitis virus-induced chemotactic cytokine. J. Biosci. 25, 47–54 (2000). https://doi.org/10.1007/BF02985181

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