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Cytological investigations on cultures of normal human blood

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Conclusions

1. In the cultures of leucocytes of normal human blood under definite conditions of cultivation there appear periods of mass multiplication of cells in mitotic fashion.

2. The mass multiplication is observed in the polyblasts (macrophages) at the stage of their mutation into stable cells, and in the fibrocytes originating in haemocultures from polyblasts.

3. For obtaining the maximal quantity of mitoses the best time for fixation is the second 24 hours after the renewal of the medium, this latter being performed not later than the end of the fourth 24 hours after the planting of the culture.

4. After appropriate fixation, the figures of divisions may be used for karyological analysis.

5. The method proposed enlarges the possibilities of the investigation of the human karyotype, and enables us to undertake the karyological investigation of any anthropogenetical or medical phenomena.

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References

  1. Chrustschoff, G.,Andres, A. andIljina-Kakujewa, W. (1931). “Kulturen von Blutleukozyten als Methode zum Studium des menschlichen Karyotypus (Vorläufige Mitteilung).”Anat. Anz. 73, H. 6/8.

  2. Timofeewski, A. andBenewolenskaja (1928). “Über Leukozytenkulturen des Menschenblutesin vitro.”Arch. exp. Zellforsch. 5.

  3. Maximov, A. (1928). “Cultures of blood leucocytes. From lymphocyte and monocyte to connective tissue.”Ibid. 5.

  4. Caffier, P. (1928). “Die prospektiven Potenzen des normalen Menschenbluts.”Ibid. 6.

  5. Hirschfeld andKlee-Rawidowitsch, E. (1928). “Untersuchungen über die Genese der Blutmakrophagen und verwandter Zellformen, sowie ihr Verhalten in den—in vitro—Kulturen. I. Normales und leukämisches Menschenblut.”Z. Krebsforsch. 27, H. 1/2.

  6. Chlopin, N. (1931). “Studien über Gewebekulturen in artfremdem Blutplasma. V. Das Verhalten und die Verwandlungen des menschlichen Mesenchyms im Explantat.”Arch. exp. Zellforsch. 12.

  7. Andres, A. andShiwago, P. (1933). “Karyologische Studien an myeloischer Leukämie des Menschen.”Folia haematol. 49, H. 1/3.

  8. Hence, A. (1929). “Comparison of mitosis in chick tissue-cultures and in sectioned embryos.”Biol. Bull. 50, No. 2.

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  9. Caffier, P. (1930). “Über das Verhältnis der Zellachse zur Achse der Teilungsspindel bei der Mitose menschlicher Zellenin vitro.”Arch. exp. Zellforsch. 10, H. 2.

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  10. Kemp, T. (1929). “Über das Verhalten der Chromosome in den somatischen Zellen des Menschen.”Z. mikr.-anat. Forsch. 16.

  11. Caffier, P. (1932). “Chromosomenstudien am menschlichen embryonalen Gewebe. Ein Beitrag zum Problem des Wachstums von Embryo und Tumor.”Z. Geburtsh. Gynäk. 101.

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Authors and Affiliations

  1. Medico-Biological Institute, Moscow

    G. K. Chrustschoff

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  1. G. K. Chrustschoff
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Chrustschoff, G.K. Cytological investigations on cultures of normal human blood. Journ. of Genetics 31, 243–261 (1935). https://doi.org/10.1007/BF02982343

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  • DOI: https://doi.org/10.1007/BF02982343

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