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Biological Significance of Proliferation, Apoptosis, Cytokines, and Monocyte/Macrophage Cells in Bone Marrow Biopsies of 145 Patients With Myelodysplastic Syndrome

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Abstract

Labeling index (LI), apoptosis, levels of 2 pro-apoptotic cytokines tumor necrosis factor-α (TNF-α) and transforming growth factor-β (TGF-β), and the number of monocyte/macrophage cells that are the likely source of the cytokines were simultaneously measured in plastic-embedded bone marrow (BM) biopsy sections of 145 patients with myelodysplastic syndromes (MDS). TNF-α was correlated with TGF-β (P = .001) and with monocyte/macrophage cells (P = .003). Patients with excess blasts in their marrows had a higher TGF-β level (P = .01) and monocyte/macrophage number (P = .05). In a linear regression model, TGF-β emerged as the most significant biological difference between patients who have excess of blasts and those who do not (P = .01). We conclude that in addition to TNF-α, TGF-β also plays a significant role in the initiation and pathogenesis of MDS, and that a more precise definition of its role will likely identify better preventive and therapeutic strategies.

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Authors and Affiliations

  1. Rush Cancer Institute, Rush-Presbyterian-St. Luke’s Medical Center, Chicago, Illinois, USA

    Krishnan Allampallam, Vilasini Shetty, Suneel Mundle, Diya Dutt, Howard Kravitz, Poluru L. Reddy, Sairah Alvi, Naomi Galili, Gurveen S. Saberwal, Shalini Anthwal, Maliha W. Shaikh, Aaron York & Azra Raza

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  1. Krishnan Allampallam
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  2. Vilasini Shetty
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  3. Suneel Mundle
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  4. Diya Dutt
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  5. Howard Kravitz
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  6. Poluru L. Reddy
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  7. Sairah Alvi
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  11. Maliha W. Shaikh
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  12. Aaron York
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  13. Azra Raza
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Correspondence to Azra Raza.

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Allampallam, K., Shetty, V., Mundle, S. et al. Biological Significance of Proliferation, Apoptosis, Cytokines, and Monocyte/Macrophage Cells in Bone Marrow Biopsies of 145 Patients With Myelodysplastic Syndrome. Int J Hematol 75, 289–297 (2002). https://doi.org/10.1007/BF02982044

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  • DOI: https://doi.org/10.1007/BF02982044

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