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Platelet-Derived Growth Factor Enhances Granulopoiesis Via Bone Marrow Stromal Cells

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Abstract

Platelet-derived growth factor (PDGF), a growth factor for connective tissue cells, stimulates erythropoiesis and megakaryocytopoiesis in vitro but the effect of PDGF on granulocyte proliferation remains unknown.The effect of the recombinant human PDGF-BB isoform on granulopoiesis was investigated in this study. The results show that PDGF significantly stimulated murine colony-forming unit-granulocyte-monocyte (CFU-GM) proliferation in a dose-dependent manner (1 to 100 ng/mL) using murine bone marrow cells (n = 4). Maximum stimulation was obtained with 50 ng/mL of PDGF (P < .01). The effect of PDGF on murine CFU-GM proliferation was compared with that of interleukin (IL)-3, IL-6, granulocyte-monocyte colony-stimulating factor (GM-CSF),and acidic fibroblast growth factor (aFGF) at their optimal doses.The stimulating activity of PDGF was higher than that of aFGF but lower than that of IL-3, IL-6, or GM-CSF. There is no synergistic effect between PDGF and IL-3 or IL-6, but a significant enhancing effect was observed in IL-3 plus IL-6. PDGF also stimulated the growth of CFU-GM with CFU-megakaryocyte in the presence of bone marrow stromal cells. We also found that PDGF had similar a effect on human CFU-GM proliferation using bone marrow mononuclear cells (MNC). However, the increase in PDGF-stimulated CFU-GM proliferation was inhibited by anti-GM-CSF, anti-IL-3, and anti-IL-6 antibodies (n = 4), suggesting that endogenously produced GM-CSF, IL-3, and IL-6 may play a role in the PDGF-induced CFU-GM proliferation. Furthermore, PDGF (1 to 100 ng/mL) did not show any effect on CFU-GM proliferation when replacing bone marrow MNC with immunomagnetic selection-enriched CD34+ cells from human cord blood (n = 5; purity, 91% +- 6.5%).This study indicates that PDGF may indirectly enhance CFU-GM proliferation by inducing the bone marrow stromal cells to produce GM-CSF, IL-3, or IL-6.

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Authors and Affiliations

  1. Department of Paediatrics, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, Australia

    Mo Yang, Karen Li, Audrey Carmen Lam, Patrick Man Pan Yuen & Tai Fai Fok

  2. Centre for Thrombosis and Vascular Research, School of Pathology, The University of New South Wales, 2052, Sydney, NSW, Australia

    Colin N. Chesterman & Beng H. Chong

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  1. Mo Yang
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  2. Karen Li
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  3. Audrey Carmen Lam
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  6. Colin N. Chesterman
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  7. Beng H. Chong
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Correspondence to Mo Yang.

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Yang, M., Li, K., Lam, A.C. et al. Platelet-Derived Growth Factor Enhances Granulopoiesis Via Bone Marrow Stromal Cells. Int J Hematol 73, 327–334 (2001). https://doi.org/10.1007/BF02981957

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  • DOI: https://doi.org/10.1007/BF02981957

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