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Pollen-wall proteins: Exine-held fractions associated with the incompatibility response in cruciferae

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Summary

During maturation of the pollen in species ofIberis (Cruciferae) materials from the anther tapetum are transferred to cavities of the exine. These sporophytically-derived materials are responsible for the rejection response induced in the stigmatic papillae within 4–6 h. following incompatible pollination, a response which leads to the occlusion of the stigma papillae by the polysaccharide, callose. The rejection reaction can be induced with agar or agarose gels into which pollen-wall materials have been allowed to diffuse, and also with isolated fragments of the tapetum itself taken from anthers of incompatible plants before transfer of its contents to the pollen. Partial fractionation of the exine-held materials using thin-layer gel filtration suggests that the incompatibility reactions are mediated by one or more “recognition” proteins or glycoproteins in a group with molecular weights in the range 10,000–25,000 daltons.

The rejection response has been found to be a rapid and sensitive assay for incompatibility in several Cruciferae.

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Literature

  1. Arens, K.: Prova de calose por meio da microscopia a luz fluorescentia e applicacoes do metode. Lilloa18, 71–75 (1949).

    Google Scholar 

  2. Bateman, A. J.: Self-incompatibility systems in angiosperms, II.Iberis amara. Heredity8, 305–332 (1954).

    Article  Google Scholar 

  3. Burnet, F. M.: Self recognition in colonial marine forms and flowering plants in relation to the evolution of immunity. Nature232, 230–234 (1971).

    Article  PubMed  CAS  Google Scholar 

  4. Dickinson, H. G., Lewis, D.: Cytochemical and ultrastructural differences between intraspecitic compatible and incompatible pollinations inRaphanus. Proc. Roy. Soc. Lond. B,183 12–38 (1973).

    Google Scholar 

  5. Frank, J. A., Paxton, J. D.: An inducer of soybean phytoalexin and its role in the resistance of soybeans toPhytophthora rot. Phytopathol.61, 954–958 (1971).

    Article  CAS  Google Scholar 

  6. Heslop-Harrison, J.: Ribosome sites and S gene action. Nature Lond.,218, 90–91 (1967).

    Article  Google Scholar 

  7. Heslop-Harrison, J.: Tapetal origin of pollen-coat substances inLilium. New Phytol.67, 779–786 (1968).

    Article  CAS  Google Scholar 

  8. Heslop-Harrison, J., Heslop-Harrison, Y., Knox, R. B., Howlett, B.: Pollen-wall proteins: ‘Gametophytic’ and ‘Sporophytic’ fractions in the pollen walls of the Malvaceae. Ann. Bot.37, 403–412 (1973).

    CAS  Google Scholar 

  9. Howlett, B. J., Knox, R. B., Heslop-Harrison, J.: Pollen-wall proteins: release of a ragweed allergen — Antigen E — from pollen grains. J. Cell Sci. (1973, in press).

  10. Knox, R. B.: Pollen-wall proteins: pollen-stigma interactions in ragweed andCosmos (Compositae). J. Cell Sci.12, 421–444 (1973).

    PubMed  CAS  Google Scholar 

  11. Knox, R. B., Heslop-Harrison, J.: Pollenwall proteins; localisation and enzymic activity. J. Cell Sci.6, 1–27 (1970).

    PubMed  CAS  Google Scholar 

  12. Knox, R. B., Heslop-Harrison, J.: Pollen-wall proteins: fate of the intine-held antigens in compatible and incompatible pollination ofPhalaris tuberosa L. J. Cell Sci.9, 239–251 (1971a).

    PubMed  CAS  Google Scholar 

  13. Knox, R. B., Heslop-Harrison, J.: Pollen-wall proteins: localisation of antigenic and allergenic proteins in the pollen-grain walls ofAmbrosia spp. (ragweeds). Cytobios4, 49–54 (1971b).

    CAS  Google Scholar 

  14. Knox, R. B., Willing, R., Ashford, A. E.: Role of pollen-wall proteins as recognition substances in interspecific incompatibility in poplars. Nature, Lond.,237, 381–383 (1972).

    Article  CAS  Google Scholar 

  15. Knox, R. B., Willing, R., Pryor, L. D.: Interspecific hybridization in poplars using recognition pollen. Silvae Genetica (1972).

  16. Kroh, M.: An electron-microscopic study of the behaviour ofCruciferae pollen after pollination. In: H. F. Linskens (ed.), Pollen Physiology and Fertilization, p. 221–224 (1964).

  17. Linskens, H. F., Kroh, M.: Inkompatibilität der Phanerogamen. Encycl. Plant Physiology, Springer-Verlag,18, 506–530 (1967).

    Google Scholar 

  18. Nasrallah, M. E., Barber, J. T., Wallace, D. H.: Self incompatibility proteins in plants: detection, genetics and possible mode of action. Heredity24, 23–27 (1969).

    Google Scholar 

  19. Radola, B. J.: Thin-layer gel filtration of proteins. I. Methods. J. Chromatogr.38, 61–77 (1968).

    Article  PubMed  CAS  Google Scholar 

  20. Sampson, D. R.: Intergeneric pollen-stigma incompatibility in the Cruciferae. Can. J. Genetics & Cytol.4, 38–49 (1962).

    Google Scholar 

  21. Stryer, L.: Fluorescence spectroscopy of proteins. Science, N. Y.,162, 526–533 (1968).

    CAS  Google Scholar 

  22. Thompson, K. F.: Self-incompatibility in marrow-stem kale,Brassica oleracea var.acephala. I. Demonstration of sporophytic system. J. Genet.55, 45–60 (1957).

    Article  Google Scholar 

  23. Thompson, K. F., Howard, H. W.: Self-incompatibility in marrow-stem kale,Brassica oleracea var.acephala. II. Methods for the recognition in inbred lines of plants homozygous for S alleles. J. Genet.56, 1–16 (1959).

    Article  Google Scholar 

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Authors and Affiliations

  1. Cell Physiology Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey, UK

    J. Heslop-Harrison, R. B. Knox & Y. Heslop-Harrison

  2. Botany School University of Melburne, 3052, Parkville, Victoria, Australia

    R. B. Knox

Authors
  1. J. Heslop-Harrison
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  2. R. B. Knox
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  3. Y. Heslop-Harrison
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Additional information

Communicated by R. Riley

On leave from Department of Botany, Australian National University, Canberra.

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Heslop-Harrison, J., Knox, R.B. & Heslop-Harrison, Y. Pollen-wall proteins: Exine-held fractions associated with the incompatibility response in cruciferae. Theoret. Appl. Genetics 44, 133–137 (1974). https://doi.org/10.1007/BF02981926

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  • DOI: https://doi.org/10.1007/BF02981926

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