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Resistance to bifenthrin and resistance mechanisms of different strains of the two-spotted spider mite (Tetranychus urticae) from Turkey

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Abstract

Nine different strains of the two-spotted spider miteTetranychus urticae Koch (Acari: Tetranychidae) were collected on cotton from Adana, Antalya, Izmir, Manisa and Urfa in Turkey. Their responses to bifenthrin were investigated using conventional bioassay and biochemical assays. LC50 and LC90 values of bifenthrin were determined for all strains by using a residual bioassay with a petri dish-spray tower. Resistance ratios were determined by comparing the samples with a standard susceptible strain, GSS. The resistance ratios of the strains ranged from <1 to 669-fold (at LC50). Of the investigated field strains, only three (two from Adana and one from Urfa) were resistant to bifenthrin. There was a correlation between esterase enzyme activity and bifenthrin resistance according to polyacrylamide gel electrophoresis and microtiter plate assays in the three resistant strains.

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References

  1. Anon. (2002) Plant Protection Products. Ministry of Agriculture, General Directorate of Protection and Control, Istanbul, Turkey.

  2. Bennett, A.L., Claassen, A. and Du Toit, C.L.N. (1997) Esterase activity as an indicator of relative insecticide resistance inAphis gossypii (Glover) (Homoptera: Aphididae).Afr. Plant Prot. 3(1):11–16.

    Google Scholar 

  3. Campos, F., Krupa, D.A. and Jansson, R. (1997) Evaluation of petri plate assay for assessment of abamectin susceptibility inTetranychus urticae (Acari: Tetranychidae).J. Econ. Entomol. 90:742–746.

    Google Scholar 

  4. Dennehy, T.J., Grafton-Cardwell, E.E., Granett, J. and Barbour, K. (1987) Practitioner-assessable bioassay for detection of dicofol resistance in spider mites (Acari: Tetranychidae).J. Econ. Entomol. 80:998–1003.

    CAS  Google Scholar 

  5. Devonshire, A.L., Devine, G.J. and Moores, G.D. (1992) Comparison of microplate esterase assays and immunoassay for identifying insecticide resistant variants ofMyzus persicae (Homoptera: Aphididae).Bull. Entomol. Res. 82:459–463.

    Google Scholar 

  6. Goka, K. and Takafuji, A. (1992) Enzyme variations among Japanese populations of the two-spotted spider mites,Tetranychus urticae Koch.Appl. Entomol. Zool. 27:141–150.

    CAS  Google Scholar 

  7. Guerrero, F.D., Pruett, J.H., Kunz, S.E. and Kammlah, D.M. (1999) Esterase profiles of diazinon-susceptible and -resistant horn flies (Diptera: Muscidae).J. Econ. Entomol. 92:286–292.

    CAS  Google Scholar 

  8. Herron, G.A., Edge, V.E., Wilson, L.J. and Rophail, J. (1998) Organophosphate resistance in spider mites (Acari: Tetranychidae) from cotton in Australia.Exp. Appl. Acarol. 22:17–30.

    Article  CAS  Google Scholar 

  9. Herron, G.A. and Rophail, J. (1998) Tebufenpyrad (Pyranica ®) resistance detected in two-spotted spider miteTetranychus urticae Koch (Acarina: Tetranychidae) from apples in Western Australia.Exp. Appl. Acarol. 22:633–641.

    Article  CAS  Google Scholar 

  10. Herron, G.A., Rophail, J. and Wilson, L.J. (2001) The development of bifenthrin resistance in two-spotted spider mite (Acari: Tetranychidae) from Australian cotton.Exp. Appl. Acarol. 25:301–310.

    Article  PubMed  CAS  Google Scholar 

  11. Hoskins, W.M. (1960) Use of the dosage-mortality curve in quantitative estimation of insecticide resistance.Misc. Publ. Entomol. Soc. Am. 2:85–91.

    Google Scholar 

  12. Hoyt, S.C., Westigard, P.H. and Croft, B.A. (1985) Cyhexatin resistance in Oregon populations ofTetranychus urticae Koch (Acarina: Tetranychidae).J. Econ. Entomol. 78:656–659.

    CAS  Google Scholar 

  13. Kabir, K.H. and Chapman, R.B. (1997) Operational and biological factors influencing responses of spider mites (Acari: Tetranychidae) to propargite by using the petri dish — Potter tower method.J. Econ. Entomol. 90:272–277.

    Google Scholar 

  14. Keena, M.A. and Granett, J. (1987) Cyhexatin and propargite resistance in populations of spider mites (Acari: Tetranychidae) from California almonds.J. Econ. Entomol. 80:560–564.

    CAS  Google Scholar 

  15. Kuwahara, M., Miyata, T., Saito, T and Eto, M. (1982) Activity and substrate specificity of the esterase associated with organophosphorus insecticide resistance in the Kanzawa spider mite,Tetranychus kanzawai Kishida (Acarina: Tetranychidae).Appl. Entomol. Zool. 17:82–91.

    CAS  Google Scholar 

  16. LeOra Software. (1994) POLO-PC: A User’s Guide to Probit or Logit Analysis. Berkeley, CA, USA.

  17. Moores, G.D., Devonshire, A.L. and Denholm, I. (1988) A microtitre plate assay for characterizing insensitive acetylcholinesterase genotypes of insecticide-resistant insects.Bull. Entomol. Res. 78:537–544.

    Article  Google Scholar 

  18. O’Brien, P.J., Abdel-Al, Y.A., Ottea, J.A. and Graves, J.B. (1992) Relationship of insecticide resistance to carboxylesterases inAphis gossypii (Homoptera: Aphididae) from Midsouth cotton.J. Econ. Entomol. 85:651–657.

    CAS  Google Scholar 

  19. Owusu, E.O., Horiike, M. and Hirano, C. (1996) Polyacrylamide gel electrophoretic assessments of esterase in cotton aphid (Homoptera: Aphididae) resistance to dichlorvos.J. Econ. Entomol. 89:302–306.

    CAS  Google Scholar 

  20. Sawicki, R.M. and Denholm, I. (1987) Management of resistance to pesticides in cotton pests.Trop. Pest Manage. 33:262–272.

    Google Scholar 

  21. Tsagkarakou, A., Navajas, M., Lagnel, J., Gutierrez, J. and Pasteur, N. (1996) Genetic variability inTetranychus urticae (Acari: Tetranychidae) from Greece: Insecticide resistance and isozymes.J. Econ. Entomol. 89:1354–1358.

    CAS  Google Scholar 

  22. Walker, J.M. (1994) Nondenaturing polyacrylamide gel electrophoresis of proteins.in: Walker, J.M. [Ed.] Methods in Molecular Biology. Vol. 32. Humana Press Inc., Totowa, NJ, USA.

    Google Scholar 

  23. Yang, X., Buschman, L.L., Zhu, K.Y. and Margolies, D.C. (2002) Susceptibility and detoxifying enzyme activity in two spider mite species (Acari: Tetranychidae) after selection with three insecticides.J. Econ. Entomol. 95:399–406.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Plant Protection Department, Faculty of Agriculture, Süleyman Demirel University, 32260, Isparta, Turkey

    Recep Ay

  2. Plant Protection Department, Faculty of Agriculture, Ankara University, 061110, Ankara, Turkey

    M. Oktay Gürkan

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  1. Recep Ay
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  2. M. Oktay Gürkan
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Correspondence to Recep Ay.

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http://www.phytoparasitica.org posting May 17, 2005.

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Ay, R., Gürkan, M.O. Resistance to bifenthrin and resistance mechanisms of different strains of the two-spotted spider mite (Tetranychus urticae) from Turkey. Phytoparasitica 33, 237–244 (2005). https://doi.org/10.1007/BF02979860

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  • DOI: https://doi.org/10.1007/BF02979860

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