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Quantification of camalexin in several accessions ofArabidopsis thaliana following inductions withPeronospora parasitica and UV-B irradiation

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Abstract

Five accessions ofArabidopsis thaliana, Ws-3, Nd-1, Ler, Col-5 and Oy-0, were inoculated withPeronospora parasitica isolate Emoy2 and the accumulation of camalexin within infected tissues was measured. The variations in camalexin accumulation in various accessions ofA. thaliana induced by a specific (P. parasitica isolate Emoy2) and a non-specific elicitor (UV-B irradiation) were investigated. Phenotypic examination of Emoy2/Oy-0 revealed that susceptibility was characterized by extensive asexual sporulation of the pathogen, whereas early restriction of the pathogen in infected plant tissues, accompanied by chlorosis and necrosis — which are associated with the hypersensitive response — was observed in Nd-1, Ws-3 and Ler. Partial resistance detected in Col-0 was characterized by low to medium sporulation of the pathogen. Camalexin was monitored by high pressure liquid chromatography (HPLC) and found to accumulate during both compatible and incompatible interactions and also following treatment with the abiotic inducer, UV-B. Among the accessions tested, Ws-3 yielded significantly more camalexin than the other accessions, regardless of which inducers (biotic or abiotic) were used. There was no significant correlation between resistance and camalexin accumulation in theA. thaliana/P. parasitica interaction. The results suggest that there is genetic variation in camalexin biosynthesis among accessions ofA. thaliana, rather than variation in types of induction.

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Authors and Affiliations

  1. Dept. of Plant Protection, Agricultural Faculty, Çanakkale Onsekiz Mart University, 17100, Çanakkale, Turkey

    Figen Mert-Turk

  2. Agricultural Sciences Department, Imperial College of Science, Technology and Medicine, TN25 5AH, Wye, Kent, UK

    Mark H. Bennett & John W. Mansfield

  3. Plant Genetic and Biotechnology Department, Horticulture Research International, CV35 9EF, Wellesbourne, Warwick, UK

    Eric B. Holub

Authors
  1. Figen Mert-Turk
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  2. Mark H. Bennett
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  3. John W. Mansfield
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  4. Eric B. Holub
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Correspondence to Figen Mert-Turk.

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http://www.phytoparasitica.org posting Dec. 16, 2002.

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Mert-Turk, F., Bennett, M.H., Mansfield, J.W. et al. Quantification of camalexin in several accessions ofArabidopsis thaliana following inductions withPeronospora parasitica and UV-B irradiation. Phytoparasitica 31, 81–89 (2003). https://doi.org/10.1007/BF02979770

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  • DOI: https://doi.org/10.1007/BF02979770

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