Summary
-
1.
The thermolability of tissue thromboplastins is markedly increased in the presence of cysteine, glutathione sulphite and hydrosulphite, but not in the presence of cysteine, ascorbate, thiosulphate or thiocyanate.
-
2.
Thermostability is increased by fluoride (and ageing), but is overcome by cysteine.
-
3.
These effects were demonstrated with tissue thromboplastins from brain, chorion and a thyroid carcinoma, and were found equally with conventional water-soluble and lipoid-type substances.
-
4.
Thermolability appears to be dependent upon the presence of free thiols or their equivalent in an enzyme catalysed reaction.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Bibliography
Biggs, R. and Macfarlane, R. G. (1962).Human Blood Coagulation, 3rd Ed., pp. 11, 30.
Boggust, W. A.et al. (1963).Irish J. Med. Set., March, p. 131.
Chargaff, E.,et al. (1944).J. biol. Chem., 156, 161.
Chargaff, E. (1945a).J. biol. Chem., 161, 389.
Chargaff, E. (19456).Adv. in Enzymology, V, p. 34.
Clarke, N. (1965).Nature, 205, 608.
Neale, F. C.et al. (1965).J. Clin. Path., 18, 359.
Quick, A. J. (1936).Amer. J. Physiol., 114, 282.
Quick, A. J. (1952).J. Lab. Clin. Med., 39, 142.
Quick, A. J. (1958).Blood Clotting Factors, 4th Internal. Congr. Biochem., Vienna, ed. Deutsch, p. 123.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Boggust, W.A., O’Meara, R.A.Q. The thermolability of tissue thromboplastins. Ir. J. Med. Sci. 41, 11–21 (1966). https://doi.org/10.1007/BF02978345
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02978345