Abstract
Fingerprinting analysis of fresh ginseng according to root age was performed using1H-NMR spectroscopy and multivariate analysis techniques. Various peaks were detected in the aliphatic (0–3 ppm), sugar (3–6 ppm), and aromatic (6–9 ppm) regions of the1H-NMR spectra of the water extracts of fresh ginseng root. The use of principal components (PCs) analysis (PCA) for metabolomic profiling allowed the large1H-NMR data set obtained for various metabolites to be reduced to PC1, PC2, and PC3. Two dimensional score plots showed clear separations with these three components at different roots ages, and explained 89.6% of the total variance. Canonical discriminant analysis identified the ginseng roots at various ages from the NMR results with over 89.9% discrimination accuracy. These results indicate that the combination of1H-NMR and PCA provides a very promising tool for the authentication and quality control of fresh ginseng roots at different ages.
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Bailey, N. J. C., Cooper, P., Hadfield, S. T., Lenz, E. M., Lindon, J. C., Nicholson, J. K., Stanley, P. D., Wilson, I. D., Wright, B., and Taylor, S. D., Application of directly coupled HPLC-NMR- MS/MS to the identification of metabolites of 5-trifluoro-methylpyridone(2-hydroxy-5-trifluoromethylpyridine) in hydroponically grown plants.J. Agric. Food Chem., 48, 42–46 (2000).
Belton, P. S., Colquhoun, I. J., Kemsley, E. K., Delgadillo, I., Roma, P., Dennis, M. J., Sharman, M., Holmes, E., Nicholson, J. K., and Spraul, M., Application of chemometircs to the1H-NMR spectra of apple juices: discrimination between apple varieties.Food Chem., 61, 207–213 (1998).
Choi, H. K., Choi, Y. H., Verberne, M., Lefeber, A. W. M., Erkelens, C., and Verpoorte, R., Metabolic fingerprinting of wild type and transgenic tobacco plants by1H-NMR and multivariate analysis technique.Phytochemistry, 65, 857–864 (2004).
Eriksson, L., Johansson, E., Kettaneh-Wold, N., and Wold, S. Multi- and Megavariate Data Analysis. Principles and Applications. Umetrics Academy, Umea, Sweden, (2001).
Ernst, E., The efficacy of herbal medicine — an overview.Fundam. Clin. Pharmacol., 19, 405–409 (2005).
Fiehn, O., Kopka, J., Dörmann, P., Altmann, T., Trethewey, R. N., and Willmitzer, L., Metabolite profiling for plant functional genomics.Nature Biotechnol., 18, 1157–1161 (2000).
Krishnan, P., Kruger, N. J., and Ratcliffe, R. G., Metabolite fingerprinting and profiling in plants using NMR.J. Exp. Bot., 56, 255–265 (2004).
Lee, C. R., Whang, W. K., Shin, C. G., Lee, H. S., Han, S. T., Im, B. O., and Ko, S. K. Comparison of ginsenoside composition and contents in fresh ginseng roots cultivated in Korea, Japan, and China at various ages.Korea J. Food Sci. Technol., 36, 847–850 (2004).
Le Gall, G., Coloquhoun, I. J., and Defernez, M., Metabolite profiling using1H NMR spectroscopy for quality assessment of green tea,Camellia sinensis (L).J. Agric. Food Chem., 52, 692–700 (2004).
Ott, K. H., Aranibar, N., Singh, B., and Stockton, Q. W., Metabolomic classifies pathway affected by bioactive compounds. Artificial neural network classification of NMR spectra of plant extracts.Phytochemistry, 62, 971–985 (2003).
Park, J. D., Rhee, D. K., and Lee, Y. H., Biological activities and chemistry of saponins from Panax ginseng C.A. Meyer.Phytochemistry Reviews, 4, 159–175 (2005).
Pauli, G. F., qNMR- a versatile concept for the validation of natural product reference compounds.Phytochem. Anal., 12, 28–42 (2001).
Raamsonk, L. M., Teusink, B., and Broadhurst, D., A functional genomics strategy that uses metabolome data to reveal the phenotype of silent mutations.Nature Biotechnol., 19, 45–50 (2001).
Sobolev, L. J., Last, R. L., and Fernie, A. R., Proton high-field NMR study of tomato juice.Mag. Res. Chem., 41, 237–245 (2003).
Sohn, E. S., Hahm, J. S., Huh, B. Y., Park, S. C., Rhim, H. K., Han, C. S., Park, C. W., Kim, H. J., and Yang, C. M., An animal experimental study of the effect of ginseng on blood pressure and plasma renin activity in spontaneously hypertensive rat.The Korean. J. Internal Med. 23, 116–119 (1980).
Sumner, L. W., Mendes, P., and Dixon, R. A., Plant metabolomics: large-scale phytochemistry in the functional genomics era.Phytochemistry, 62, 817–836 (2003).
Tang, W. and Eisenbrand, G., Chinese Drugs of Plant Origin: Chemistry, Pharmacology, and Use in Traditional and Morden Medicine. Springer-Verlag, Berlin, (1992).
Yun, T. K., Lee, Y. S., Lee, Y. H., Kim, S. I., and Yun, H. Y., Anticarcinogenic effect ofPanax ginseng C.A. Meyer and identification of active compounds.J. Korean Med. Sci., 16, 6–18 (2001).
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Shin, YS., Bang, KH., In, DS. et al. Fingerprinting analysis of fresh ginseng roots of different ages using1H-NMR spectroscopy and principal components analysis. Arch Pharm Res 30, 1625–1628 (2007). https://doi.org/10.1007/BF02977333
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DOI: https://doi.org/10.1007/BF02977333