Abstract
The antioxidant activity ofEcklonia stolonifera was determined by measuring lipid peroxide produced when a mouse liver homogenate was exposed to the air at 37°C, using 2-thiobarbituric acid (TBA) and the radical scavenging effect on 1,2-diphenyl-2-picrylhydrazyl (DPPH) radical. The methanol extract ofEcklonia stolonifera showed strong antioxidant activity. And the methanol extract was fractionated with several solvents. With regard their fractions, the antioxidative activity were in the order of ethyl acetate>dichloromethane insoluble intermediated phase>dichloromethane>n-butanol>water fraction. The ethyl acetate soluble fraction exhibiting the strongest antioxidant activity was further purified by repeated silica gel and Sephadex LH-20 column chromatography. Antioxidant phloroglucinol was isolated and identified by1H-NMR and13C-NMR. Its antioxidant activity was similar to that of L-ascorbic acid.
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Lee, JH., Park, JC. & Choi, JS. The antioxidant activity ofEcklonia stolonifera . Arch. Pharm. Res. 19, 223–227 (1996). https://doi.org/10.1007/BF02976894
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DOI: https://doi.org/10.1007/BF02976894