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Modulation of tnf-α-induced icam-1 expression, no and h202 production by alginate, allicin and ascorbic acid in human endothelial cells

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Abstract

Plant nutrients are believed to provide protection against various diseases including inflamma-tion. Since interactions of the cell adhesion molecules are known to play important roles in mediating inflammation, inhibiting adhesion protein upregulation is a possible therapeutic tar-get. In this study, the interacellular adhesion molecule-1 (ICAM-1) was induced in human umbilical endothelial cells (HUVECs) after stimulation with TNF-α. In addition, alginate, ascor-bic acid and allicin were demonstrated to inhibit the TNF-α induced expression of ICAM-1 on the HUVECs in a dose-dependent manner. These compounds also inhibited the production of NO and H202 induced by TNF-α, which suggests that the inhibition of ICAM-1 expression by the three compounds may be due to the modulated production of the reactive oxygen/nitrogen components. Overall, these results indicate that these dietary components have a therapeutic potential in the treatment of various inflammatory disorders associated with an increase in endothelial leukocyte adhesion molecules.

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References

  • Aoki, T., Suzuki, Y. Nishio, K., Suzuki, K., Miyata, A., Oyamada, Y., Mori, M., Fujita, H., and Yamaguchi, K., Effect of antioxidants on hyperoxia-induced ICAM-1 expression in human endothelial cells.Adv. Exp. Med. Biol., 411, 503–511 (1997).

    PubMed  CAS  Google Scholar 

  • Bence, A. K., Worthen, D. R., Adams, V. R., and Crooks, P. A., The antiproliferative and immunotoxic effects of L-canavanine and L-canaline.Anticancer Drug, 13(3), 313–320 (2002)

    Article  CAS  Google Scholar 

  • Baeuerle, P. A. and Henkel, T. Function and activation of IMF- kappa B in the immune system.Annu. Rev. Immunol., 12, 141–179 (1994).

    PubMed  CAS  Google Scholar 

  • Berliner, J. A., Navab M., Fogelman, A. M., Frank, J. S., Demer, L. L., Edwards, P. A., Watson, A. D., and Lusis, A. J., Atherosclerosis: basic mechanisms, oxidation, inflammation, and genetics.Circulation, 91(9), 2488–2496 (1995).

    PubMed  CAS  Google Scholar 

  • Bevilacqua, M.P., Pober, J. S., Wheeler, M. E., Cotran, R. S., and Gimbrone, M. A. Interleukin 1 acts on cultured human vascular endothelium to increase the adhesion of polymor- phonuclear leukocytes, monocytes and related cell lines.J. Clin. Invest, 76, 2003–2009 (1985).

    Article  PubMed  CAS  Google Scholar 

  • Bevilacqua, M. P., Stengelin, S., Gimbrone, M. A., and Seed, B. Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins.Science, 243, 1160–1165 (1989).

    Article  PubMed  CAS  Google Scholar 

  • Bordia, T. and Mohammed, N., Thomson, M., An evaluation of garlic and onion as antithrombotic agents.Protaglandins Leukot. Essent. Fatty Acids, 54, 183–186 (1996)

    Article  CAS  Google Scholar 

  • Brostjan, C., Anrather, J., Csizmadia, V., Natrajan, G., and Winkler, H., Glucocorticoids inhibit E-selectin expression by targeting NF-kB and not ATF/c-jun.J. Immunol., 158, 3836–3844 (1997).

    PubMed  CAS  Google Scholar 

  • Carlos, T. and Harlan, J. M., Leukocyte-endothelial interactions.Blood, 84, 2068–2101 (1994)

    PubMed  CAS  Google Scholar 

  • Cellini, L., Di Campli, E., and Masulli, M., Inhibition of Helico- bacter pylori by garlic extracts (Allium sativum).FEMS Immunol. Med. Microbiol., 13, 273–277 (1996).

    Article  PubMed  CAS  Google Scholar 

  • Chang, M. L. W. and Johnson, M. A., Effect of garlic on carbohydrate metabolism and lipid synthesis in rats.J. Nutr., 110, 931–936, 1980.

    PubMed  CAS  Google Scholar 

  • Chen, C., Chou, C., Sun, Y., and Huang, W., Tumor necrosis factor α-induced activation of downstream NF-kB site of the promoter mediates epithelial ICAM-1 expression and monocyte adhesion: involvement of PKCα, tyrosine kinase, and IKK2, but not MAPKs, pathway.Cell Signal., 13, 543–553 (2001)

    Article  PubMed  CAS  Google Scholar 

  • Cobb, R. R., Felts, K. A., Mckenzie, T. C., Parry, G., and Mackman, N., A benxothiophene-carboxamide is a potent inhibitor of IL-1 β induced VCAM-1 gene expression in human endothelial cells.FEBS Lett., 382, 323–326 (1996).

    Article  PubMed  CAS  Google Scholar 

  • Cominacini, L., Pasini, A., Garbin, U., Evangelista, S., Crea, A., Tagliacozzi, D., Nava, C., Davoli, A., and LoCascio, V., Zofenopril inhibits the expression of adhesion molecules on endothelial cells by reducing reactive oxygen species.Am. J. Hypertens., 15, 891–895 (2002).

    Article  PubMed  CAS  Google Scholar 

  • Darr, D., Dunston, S., Faust, H., and Pinnell, S., Effectiveness of antioxidants (vitamin C and E) with and without sunscreens as topical photoprotectants.Acta Derm. Venereol., 76, 264–268 (1996)

    PubMed  CAS  Google Scholar 

  • Davidson, I. W., Sutherland, Z. W., and Lawson, C. J., Purification and Properties of an alginate lyase from a marine bacterium.Biochem. J., 159, 707–713 (1976).

    PubMed  CAS  Google Scholar 

  • Ding, A. H., Nathan, C. F., and Stuehr, D. J., Release of reactive nitrogen intermediates and reactive oxygen intermediated from mouse peritoneal macrophages. Comparison of activating cytkines and evidence for independent production.J. Immunol., 141, 2407–2412 (1988).

    PubMed  CAS  Google Scholar 

  • Eastwood, M. A., Interaction of dietary antioxidants in vivo: how fruit and vegetables prevent disease.QJM, 92, 527–530 (1999).

    Article  PubMed  CAS  Google Scholar 

  • Eilat, S., Oestraicher, Y., Rabinkov, A., Alteration of lipid profile in hyperlipidemic rabbits by allicin, an active constituent of garlic.Coronay Artery Dis., 6, 985–990 (1995).

    CAS  Google Scholar 

  • Fotsis, T., Pepper, M. S., Aktas, E., Breit, S., Rasku, S., Adlercreutz, H., Wahala, K., Montesano, R., and Schweigerer, L., Flavonoids, dietary-derived inhibitors of cell proliferation an.in vitro angiogenesis.Cancer Res., 57, 2916–2921 (1997).

    CAS  Google Scholar 

  • Foushee, D. B., Ruffin, J., and Banerjee, U., Garlic as a natural agent for the treatment of hypertension: A preliminary report.Cytobios., 34, 145–152 (1982).

    PubMed  CAS  Google Scholar 

  • Hasegawa, T., Takahashi, T., Inada, Y., Yamada, C., and Tanaka, Y., Reparative effects of sodium alginate (Alloid G) on radiation stomatitis.Nippon Igaku Hoshasen Gakkai Zasshi, 49, 1047–1051 (1989).

    PubMed  CAS  Google Scholar 

  • Hensley, K., Robinson, K. A., Gabbita, S. P., Salsman, S., and Floyd, R. A., Reactive oxygen species, cell signaling, and cell injury.Free Radic. Biol. Med., 28, 1456–1462 (2000).

    Article  PubMed  CAS  Google Scholar 

  • Hobauer, R., Frass, M., Gmeiner, B., Kaye, A. D., and Frost, E. A., Garlic extract (allium sativum) reduces migration of neutrophils through endothelial cell monolayers.Middle East J. Anesthesiol., 15, 649–658 (2000).

    PubMed  CAS  Google Scholar 

  • Johnson, D. R., Douglas, I., Jahnke, A., Ghosh, S., and Pober, J. S., A sustained reduction in IkB-β may contribute to persistent NF-kB activation in human endothelial cells.J. Biol. Chem., 271, 16317–16322 (1996).

    Article  PubMed  CAS  Google Scholar 

  • Middleton, E., Effect of plant flavonoids on immune and inflammatory cell function.Adv. Exp. Med. Biol., 439, 175–182 (1998)

    PubMed  CAS  Google Scholar 

  • Mcmahon, F. G. and Vargas, R., Can garlic lower blood pressure? A pilot study.Pharmacotherapy, 13, 406–407 (1993).

    PubMed  CAS  Google Scholar 

  • Moncada, S., The L-arginine:nitric oxide pathway.Acta Physiol. Scand., 145, 201–227 (1992).

    PubMed  CAS  Google Scholar 

  • Morandini, R., Boeynaems, J. M., Duhant, X., Jacquemotte, F., Kinnaert, E., and Ghanem, G., SODs are involved in the regulation of ICAM-1 expression in human melanoma and endothelial cells.Cell. Mol. Biol., 45, 1053–1063 (1999).

    PubMed  CAS  Google Scholar 

  • Myers, C. L., Wetherimer, S. J., Schembri-King, J., Paeks, T., and Wallace, R. W., Induction of ICAM-1 by TNF-alpha, IL-1 beta, and LPS in human endothelial cells after downre- gulation of PKC.Am. J. Physiol., 263, C767-C772 (1992).

    PubMed  CAS  Google Scholar 

  • Neuner, P., Klosner, G., Pourmojib, M., Knobler, R., and Schwarz, T., Pentoxifylline in vivo and in vitro down-regulates the expression of the intercellular adhesion molecule-1 in monocytes.Immunology, 90, 435–439 (1997).

    Article  PubMed  CAS  Google Scholar 

  • Peng, Q., Wei, Z., and Lau, H. S., Pycnolgenol inhibits tumor necrosis factor-α-induced nuclear factor kappa B activation and adhesion molecule expression in human vascular endothelial cells.Cell. Mol. Life Sci., 57, 834–841 (2000).

    Article  PubMed  CAS  Google Scholar 

  • Pinto, J. T. and Rivlin, R. S., Antiproliferative effects of allium derivatives from garlic.J. Nutr., 131, 1058S-1060S (2001).

    PubMed  CAS  Google Scholar 

  • Prasad, K., Laxdal, V. A., and Yu, M., Antioxidant activity of allicin, an active principle in garlic.Mol. Cell Biochem., 148, 183–189 (1995).

    Article  PubMed  CAS  Google Scholar 

  • Redlich, C. A., Rockwell, S., Chung, J. C., Sikora, A. G., Kelley, M., and Mayne, S. T., Vitamin A inhibits radiation-induced pneumonitis in rats.J. Nutr., 128, 1661–1664 (1998).

    PubMed  CAS  Google Scholar 

  • Sherman, M. L., Datta, R., Hallahan, D. E., Weicheslbaum, R. R., and Kufe, D. W., Regulation of tumor necrosis factor gene expression by ionizing radiation in human myeloid leukemia cells and peripheral blood monocytes.J. Clin. Invest., 81, 506–510 (1991).

    Google Scholar 

  • Son, E. W., Cho, C. K., Rhee, D. K., and Pyo, S., Inhibition of gamma-irradiation induced adhesion molecules and NO production by alginate in human endothelial cells.Arch. Pharm. Res., 24, 466–471 (2001).

    Article  PubMed  CAS  Google Scholar 

  • Soon-Shiong, P., Henitz, R. E., Merideth, N., Yao, Q. X., Zheng, T., Murphy, M., Moloney, M. K., Schmehl, M., and Harris, M., Insulin independence in a type I diabetic patient after encapsulated islet transplantation.Lancet, 343, 950–951 (1994).

    Article  PubMed  CAS  Google Scholar 

  • Spiecker, M., darius, H., kaboth, K., Hubner, F., and Liao, J. K., Differential regulation of endothelial cell adhesion molecule expression by nitric oxide donors and antioxidants.J. Leuk. Biol., 63, 732–739 (1998).

    CAS  Google Scholar 

  • Springer, T. A., Adhesion receptors of the immune system.Nature, 346, 425–434 (1990).

    Article  PubMed  CAS  Google Scholar 

  • Strain, J. J. and Benzie, I. F. F, Antioxidant: diet and antioxidant defence: In Sadler, M., Strain, J. J., Cabellero, B. (Eds)The Encyclopedia of Human Nutrition. Academic Press, London, pp. 95–106 (1997).

    Google Scholar 

  • Tze, W. J. and Tai, J., Biocompatibility and immunological studies of microencapsulation with cross-linked alginate capsules.Transplantation, 33, 563–564 (1982).

    Article  PubMed  CAS  Google Scholar 

  • Wan, C. P., Myung, E., and Lau, B. H., An automated micro- fluorometric assay for monitoring oxidative burst activity of phagocytes.J. Immunol. Methods, 159, 131–138 (1993).

    Article  PubMed  CAS  Google Scholar 

  • Weber, C. Erl, W., Pietsch, A., and Weber, P. C., Aspirin inhibits nuclear factor-kB mobilization and monocyte adhesion in stimulated human endothelial cells.Circulation, 91, 1914–1917 (1995).

    PubMed  CAS  Google Scholar 

  • Weiser, M. R., Gibbs, S., and Hechtman, H. B., Strategies to inhibit cellular adhesion molecules: In Paul, L. C. and Issekutz, T. (Eds.)Adhesion molecules in Health and Disease. Marcel Dekker Inc., New York, pp. 55–86, (1997).

    Google Scholar 

  • Yeh, Y. Y. and Yeh, S. M., Garlic reduces plasma lipids by inhibiting hepatic cholesterol and triacylglycerol synthesis.Lipids, 29, 189–193 (1994).

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Division of Immunopharmacology, College of Pharmacy, Sungkyunkwan University, 440-746, Suwon, Kyunggi-do, Korea

    Sung -Ji Mo, Eun -Wha Son, Dong -Kwon Rhee & Suhkneung Pyo

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  1. Sung -Ji Mo
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  2. Eun -Wha Son
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  3. Dong -Kwon Rhee
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Mo, S.J., Son, E.W., Rhee, D.K. et al. Modulation of tnf-α-induced icam-1 expression, no and h202 production by alginate, allicin and ascorbic acid in human endothelial cells. Arch Pharm Res 26, 244–251 (2003). https://doi.org/10.1007/BF02976837

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