Skip to main content
Log in

Microencapsulation of water-soluble isoflavone and physico-chemical property in milk

  • Research Articles
  • Articles
  • Published:
Archives of Pharmacal Research Aims and scope Submit manuscript

Abstract

This study was carried out to investigate the addition of water-soluble isoflavone into milk by means of microencapsulation technique. The yield of microencapsulation, sensory attributes, and capsule stability of water-soluble isoflavone microcapsules in milk were measured. Coating materials used was polyglycerol monostearate (PGMS), and core material was water-soluble isoflavone. The encapsulation yield of water-soluble isoflavone with PGMS was 67.2% when the ratio of coating material to core material was 15:1. The rate of water-soluble isoflavone release from capsules was 18, 19, and 25% when stored at 4, 20, and 30°C for 12 days in milk, respectively. In sensory evaluation, beany flavor and color of microencapsuled water-soluble isoflavone added milk were significantly different from uncapsuled water-soluble isoflavone added milk, however, bitterness was not significantly different.In vitro study, micro-cap-sules of water-soluble isoflavone in simulated gastric fluid with the range of 3 to 6 pHs were released 3.0>15.0%, however, the capsules in simulated intestinal fluid with pH 7 were released 95.7% for 40 min incubation time. In conclusion, this study provided that PGMS as coating materials was suitable for the microencapsulation of water-soluble isoflavone, and the capsule containing milk was almost not affected with sensory attribute.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Adlercreutz, H., Phytoestrogens: epidemiology and a possible role in cancer protection.Environ Health. Perspect., 103 (Suppl. 7), 103–112 (1995).

    Article  PubMed  CAS  Google Scholar 

  • Ahn, J. and Kwak, H. S., Optimizing cholesterol removal in cream using β-cyclodextrin and response surface methodology.J. Food Sci., 64, 629–632 (1999).

    Article  CAS  Google Scholar 

  • Anthony, M. S., Clarkson, T. B., Hughes, C. L., Morgan, T. M., and Burke, G. L., Soybean isoflavones improve cardiovascular risk factor without affecting the reproductive system of peripubertal rhesus monkeys.J. Nutr., 126, 43–50 (1996).

    PubMed  CAS  Google Scholar 

  • Arliss, R. M. and Biermann, C. A., Do soy isoflavones lower cholesterol, inhibit atherosclerosis, and play a role in cancer prevention?Holist Nurs. Pract, 16(5), 40–48 (2002).

    PubMed  Google Scholar 

  • Berseníeva, E. A., Inanov, A. A., Sansonova, T. P., Chernova, E. M., and Oragvelidze, E. M., Microencapsulated aromatizers for tea.Pishchevaya Paomyshlennost USSR, 1, 57–59 (1990).

    Google Scholar 

  • Braun, S. D. and Olson, N. F., Encapsulation of proteins and peptides in milk fat. Encapsulation efficiency and temperature and freezing stability.J. Microencapsulation, 3(2), 115–126 (1986).

    Article  PubMed  CAS  Google Scholar 

  • Cassidy, A., Bingham, S., and Setchell, K. D., Biological effects of a diet of soy protein rich in isoflavones on the menstrual cycle of premenopausal women.Am. J. Clin. Nutr., 60, 333–340 (1994).

    PubMed  CAS  Google Scholar 

  • Cassidy, A., Bingham, S., and Setchell, K. D. R., Biological effects isoflavones in young women-importance of the chemical composition of soya products.Br. J. Nutr., 74, 547–560 (1995).

    Article  Google Scholar 

  • Fanti, P., Monier-Faugere, M. C., Geng, Z., Schmidt, J., Morris, P. E., Cohen, D., and Malluche, H. H., The phytoestrogen genistein reduces bone loss in short-term ovariectomized rats.Osteoporos Int., 8(3), 274–281 (1998).

    Article  PubMed  CAS  Google Scholar 

  • Freund, O. J., Amedee, D. R. and Laversanne, R.,In vitro andin vivo stability of new multilamellar vesicles.Life Sci., 67, 411–419 (2000).

    Article  PubMed  CAS  Google Scholar 

  • Fukui, K., Tachibana, N., Wanezaki, S., Tsuzaki, S., Takamatsu, K., Yamamoto, T., Hashimoto, Y., and Shimoda, T., Isoflavone-free soy protein prepared by column chromatography reduces plasma cholesterol in rats.J. Agric. Food Chem., 25,50(20), 5717–5721 (2002).

    Article  CAS  Google Scholar 

  • Grundy, S. M., Bilheimer, D., Blackburn, H., Brown, W. V., Kwiterovich, P. O. Jr., Mattson, R., Schonfeld, G., and Weidman, W. H., Rationale of the diet-heart statement of the American Heart Association. Report of Nutrition Committee.Circulation, 65(4), 839A-854A (1982).

    PubMed  CAS  Google Scholar 

  • Gurr, M. I., Dietary lipids and coronary heart disease: old evidence, new perspective.Prog. Lipid Res., 31(3), 195–243 (1992).

    Article  PubMed  CAS  Google Scholar 

  • Jackson, L. S. and Lee, K., Microencapsulated iron for food fortification.J. Food Sci., 56, 1047–1050 (1991).

    Article  CAS  Google Scholar 

  • Kwak, H. S., Ihm, M. R., and Ahn, J., Microencapsulation of β- galactosidase with fatty acid esters.J. Dairy Sci., 84, 1576–1582 (2001).

    PubMed  CAS  Google Scholar 

  • Kwak, H. S., Nam, C. G., and Ahn, J., Low cholesterol Mozzarella cheese obtained from homogenized and β-cyclodextrin-treated milk.Asian-Aust. J. Anim. Sci., 14(2), 268–275 (2001).

    CAS  Google Scholar 

  • Kwak, H. S., Kwon, S. H., Lee, J. B., and Ahn, J.,In vitro stability of β-galactosidase microcapsules.Asian-Aust. J. Anim. Sci., 15(12), 1808–1818 (2002).

    CAS  Google Scholar 

  • Lee, D. K., Ahn, J., and Kwak, H. S., Cholesterol removal from homogenized milk with β-cyclodextrin.J. Dairy Sci., 82, 2327–2330 (1999).

    PubMed  CAS  Google Scholar 

  • Magee, E. L. Jr. and Olson, N. F., Microencapsulation of cheese ripening systems: Formation of microcapsules.J. Dairy Sci., 64, 600–610 (1981a).

    Article  CAS  Google Scholar 

  • Magee, E. L. Jr. and Olson, N. R., Microencapsulation of cheese ripening systems: Stability of microcapsules.J. Dairy Sci., 64, 611–615 (1981b).

    CAS  Google Scholar 

  • Setchell, K. D. R. and Cassidy, A., Dietary isoflavones: Biological effects and relevance to human health.J. Nutr., 129, 758S-767S (1999).

    PubMed  CAS  Google Scholar 

  • Szejtli, J., Chapter 2: Cyclodextrin inclusion selected treatments for accelerating ripening of buffalo milk Cheddar cheese.Ph. D.Thesis. Gujarat Agricultural University Anand India (1988).

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Hae Soo Kwak.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Seok, J.S., Kim, J.S. & Kwak, H.S. Microencapsulation of water-soluble isoflavone and physico-chemical property in milk. Arch Pharm Res 26, 426–431 (2003). https://doi.org/10.1007/BF02976702

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02976702

Key words

Navigation