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Costunolide induces differentiation of human leukemia HL-60 cells

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Abstract

Costunolide has been reported to be a cytotoxic and chemopreventive agent. This work investigated the mechanism of the antiproliferative effect of costunolide and determined that it induced differentiation of the human leukemia cell line HL-60. Costunolide exhibited a potent antiproliferative activity against HL-60 cells. It was also found to be a potent inducer of differentiation in human leukemia derived HL-60 cells through the examination of differentiation markers, as assessed by the reduction of nitroblue tetrazolium, the increase in esterase activities and phagocytic activity, morphology change and the expression of CD14 and CD66b surface antigens. These results, accompanied by a decline in the expression of c-myc protein, suggest that costunolide induces differentiation of human leukemia cells to granulocytes and monocytes/macrophages lineage.

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Authors and Affiliations

  1. College of Pharmacy, Kyung Hee University, 130-701, Seoul

    Jung-Hye Choi, Bo-Rim Seo, Seong-Hoon Seo & Kyung-Tae Lee

  2. College of Medicine, Kyung Hee University, 130-701, Seoul

    Jae-Hoon Park

  3. Division of Applied Plant Sciences, Sangji University, 220-702, Wonju

    Hee-Juhn Park

  4. College of Pharmacy, Kyung-Sung University, 608-736, Pusan, Korea

    Jong-Won Choi

  5. Department of Hospital Pharmacy, School of Medicine, Kanazawa University, 13-1 Takara-machi, 920-8641, Kanazawa, Japan

    Yoshie Itoh & Ken-ichi Miyamoto

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  1. Jung-Hye Choi
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  2. Bo-Rim Seo
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  3. Seong-Hoon Seo
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  4. Kyung-Tae Lee
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  5. Jae-Hoon Park
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  6. Hee-Juhn Park
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  7. Jong-Won Choi
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  9. Ken-ichi Miyamoto
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Correspondence to Kyung-Tae Lee.

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Choi, JH., Seo, BR., Seo, SH. et al. Costunolide induces differentiation of human leukemia HL-60 cells. Arch Pharm Res 25, 480–484 (2002). https://doi.org/10.1007/BF02976606

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