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P63 expression in normal, hyperplastic and malignant breast tissues

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Abstract

Background

p63 is a homologue of the p53 tumor suppressor gene and its protein is selectively expressed in the basal cells of a variety of epithelial tissues. It has recently been confirmed that p63 is expressed in the basal cells of normal prostate glands but not in prostatic carcinomas. Whether expression of p63 in breast correlates with tumor progression is the focus of this study.

Methods

Forty cases, which all contained normal breast tissue, ductal hyperplasia, ductal carcinomain situ and invasive ductal carcinoma in the same patient were included in this investigation using an indirect immunohistochemical method and double staining.

Results

p63 was exclusively expressed in the myoepithelial cells of normal breast, partially expressed in ductal hyperplasia, rarely expressed in carcinomain situ and not expressed in invasive carcinomas.

Conclusions

The results suggest an association between loss of p63 expression and progression of breast ductal carcinoma. p63 immunostaining might be of assistance for distinguishing invasive ductal carcinoma from ductal carcinomain situ or rare questionable ductal hyperplastic lesions, leading to correct therapy clinically.

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Abbreviations

DCIS:

Ductal carcinomain situ

SMA:

Smooth muscle actin

References

  1. Yang A, Kaghad M, Wang Y,et al: p63, a p53 homolog at 3q27-29, encodes multiple products with transactivating, death-inducing, and dominant-negative activities.Mol Cell 2: 305–316, 1998.

    Article  PubMed  CAS  Google Scholar 

  2. Parsa R, Yang A, McKeon F,et al: Association of p63 with proliferative potential in normal and neoplastic human keratinocytes.J Invest Dermatol 113: 1099–1105, 1999.

    Article  PubMed  CAS  Google Scholar 

  3. Signoretti S, Waltregny D, Dilks J,et al: p63 is a prostate basal cell marker and is required for prostate development.Am J Pathol 157: 1769–1775, 2000.

    PubMed  CAS  Google Scholar 

  4. Tavassoli FA: Ductal intraepithelial neoplasia (IDH, AIDH and DCIS).Breast Cancer 7: 315–320, 2000.

    Article  PubMed  CAS  Google Scholar 

  5. Trink B, Okami K, Wu L,et al: A new human p53 homologue.Nat Med 248: 603–607, 1998.

    Google Scholar 

  6. Strano S, Rossi M, Fontemaggi G,et al: From p63 to p53 across p73.FEBS Lett 16: 163–170, 2001.

    Article  Google Scholar 

  7. Yang A, Schweitzer R, Sun D,et al: p63 is essential for regenerative proliferation in limb, craniofacial and epithelial development.Nature 398: 714–718, 1999.

    Article  PubMed  CAS  Google Scholar 

  8. Mills AA, Zheng B, Wang XJ,et al: p63 is p53 homologue required for limb and epidermal morphogenesis.Nature 398: 708–713, 1999.

    Article  PubMed  CAS  Google Scholar 

  9. Gaiddon C, Lokshin M, Ahn J,et al: A subset of tumor-derived mutant forms of p53 down-regulate p63 and p73 through a direct interaction with the p53 core domain.Mol Cell Biol 21: 1874–1887, 2001.

    Article  PubMed  CAS  Google Scholar 

  10. De Laurenzi V, Melilno G: Evolution of functions within the p53/p63/p73 family.Ann N Y Sci 926: 90–100, 2000.

    Article  Google Scholar 

  11. Osborne MP: Breast anatomy and development. In: Harris, Jay R eds, Diseases of the Breast, 2nd ed, Lippincott Williams & Wilkins, Philadelphia, p9, 2000.

    Google Scholar 

  12. Barbareschi M, Pecciarini L, Cangi MG,et al: p63, a p53 homologue, is a selective nuclear marker of myoepithelial cells of the human breast.Am J Surg Pathol 25: 1054–1060, 2001.

    Article  PubMed  CAS  Google Scholar 

  13. Kaelin WGJ: The emerging p53 gene family.J Natl Cancer Inst 91: 594–594, 1999.

    Article  PubMed  CAS  Google Scholar 

  14. Yang A, McKeon F, Wang Y,et al: p63 and p73: p53 mimics, menaces and more.Nature Rev Mol Cell Biol 1: 194–207, 2000.

    Google Scholar 

  15. Gottlieb C, Raju U, Greenwald KA: Myoepithelial cells in the differential diagnosis of complex benign and malignant breast lesions: an Immunohistochemical study.Mod Pathol 3: 134–140, 1990.

    Google Scholar 

  16. Xiaojuan W, Ichiro M, Weihua T,et al: Metaplastic carcinoma of the Breast: p53 analysis identified the same point mutation in the three histologic components.Mod Pathol 14: 1183–1186, 2001.

    Article  Google Scholar 

  17. Glickman JN, Yang A, Shahsafaei A,et al: Expression of p53-related protein p63 in the gastrointestinal tract and in esophageal metaplastic and neoplastic disorders.Hum Pathol 32: 1157–1165, 2001.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Pathology, Wakayama Medical University, Japan

    Xiaojuan Wang, Ichiro Mori, Weihua Tang, Misa Nakamura, Yasushi Nakamura, Misako Sato & Kennichi Kakudo

  2. Department of Surgery, Kihoku Affiliated Hospital, Wakayama Medical University, Japan

    Takeo Sakurai

Authors
  1. Xiaojuan Wang
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  2. Ichiro Mori
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  3. Weihua Tang
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  4. Misa Nakamura
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  5. Yasushi Nakamura
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  6. Misako Sato
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  7. Takeo Sakurai
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  8. Kennichi Kakudo
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Corresponding author

Correspondence to Xiaojuan Wang.

Additional information

Reprint requests to Xiaojuan Wang, Department of Pathology, Wakayama Medical University, 811-1 Kimiidera, Wakayama City, Wakayama 641-001 2, Japan.

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Wang, X., Mori, I., Tang, W. et al. P63 expression in normal, hyperplastic and malignant breast tissues. Breast Cancer 9, 216–219 (2002). https://doi.org/10.1007/BF02967592

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  • DOI: https://doi.org/10.1007/BF02967592

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