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Complete nucleotide sequence of mouse mammary tumor virus from jyg chinese wild mice: Absence of bacterial insertion sequences in the cloned viral gag gene

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Abstract

Mammary tumors of a newly isolated strain of Chinese wild mouse (JYG mouse) harbor exogenous mouse mammary tumor virus (MMTV). The complete nucleotide sequence of exogenous JYG-MMTV was determined on the proviral 5′ long terminal repeat (LTR) (partial)-gag- pol- env- 3′ LTR (partial) fragment cloned into a plasmid vector and the cDNA sequence from JYG-MMTV producing cells. Similarly to the other MMTV species the LTR of JYG-MMTV contains an open reading frame (ORF). The amino acid sequence of the JYG-MMTV ORF resembles that of SW-MMTV (92% identity) and endogenous Mtv-7 (93% identity) especially at the C-terminal region. Thus, a functional similarity in T-cell receptor Vß recognition as a superantigen is implicated among these MMTV species. Analysis of the viralgag nucleotide sequence revealed that this gene is not disrupted by the bacterial insertion sequence IS1 or IS2, which have been reported to be present in the majority of the plasmids containing thegag region. Comparison of amino acid sequences of JYG-MMTV with those of BR6-MMTV showed that over 96% of the amino acids ofgag, pol, protease andenv products are identical. These results suggest the intact nature of the nucleotide sequence of the near full-length MMTV genome cloned in the plasmid.

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Abbreviations

LTR:

Long terminal repeat

Mls antigen:

Minor lymphocyte stimulating antigen

MMTV:

Mouse mammary tumor virus

ORF:

Open reading frame

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Authors and Affiliations

  1. Research Laboratory, Zenyaku Kogyo Co Ltd, 2-33-8 Oizumi, Nerima-ku, 178, Tokyo, Japan

    Masahiro Nishio, Masato Sasaki & Jumpei Enami

  2. Departments of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta

    Lai Xu

  3. Departments of Immunology and Microbiology, Medical College of Georgia, Augusta

    Nurul H Sarkar

  4. Departments of Anatomy, Nara Medical University, Japan

    Satomi Haga

  5. Departments of Pathology, Nara Medical University, Japan

    Shunsuke Imai

  6. Department of Technology and Applied Science, Osaka Prefecture University, Japan

    Masaaki Okumoto & Nobuko Mori

  7. Institute of Biochemistry, University of Lausanne, Epalinges

    Hans Acha-Orbea

Authors
  1. Masahiro Nishio
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  2. Lai Xu
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  3. Masato Sasaki
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  4. Satomi Haga
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  5. Masaaki Okumoto
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  7. Nurul H Sarkar
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  8. Hans Acha-Orbea
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  9. Jumpei Enami
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  10. Shunsuke Imai
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Nishio, M., Xu, L., Sasaki, M. et al. Complete nucleotide sequence of mouse mammary tumor virus from jyg chinese wild mice: Absence of bacterial insertion sequences in the cloned viral gag gene. Breast Cancer 1, 89–94 (1994). https://doi.org/10.1007/BF02967037

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  • DOI: https://doi.org/10.1007/BF02967037

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