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Diagnosis of ductal carcinoma in situ (DCIS) and intraductal papilloma using fluorescence in Situ Hybridization (FISH) analysis

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Abstract

Background

It is often difficult to pre-operatively diagnose ductal carcinoma in situ (DCIS) or intraductal papilloma (IDP). Current reports show that breast cancer frequently has numerical aberrations of chromosomes 1, 11 and 17. We investigated whether fluorescence in situ hybridization (FISH) analysis using three centromere-specific probes for chromosomes 1, 11 and 17 was fesible for diagnosing intraductal breast lesions.

Methods

Fine-needle aspiration specimens from 102 breast lesions including DCIS (10), invasive ductal carcinoma (IDC) (78), IDP (7), fibroadenoma (6) and mastopathy (1) were examined for numerical aberrations on chromosomes 1, 11, 17 using FISH. If over 15% of all cells showed one signal, the sample was judged monosomic. If over 20% of cells showed three or more signals, it was considered polysomic. If the specimen had an aberration of at least one chromosome, it was judged positive.

Result

Nine of 10 DCISs showed numerical aberrations of at least one chromosome whereas 65 of 78 IDCs and 2 of 14 benign lesions (containing 7 IDPs of which one case was positive) showed numerical aberrations on these chromosomes. The proportion of positive results was highest with DCIS. Moreover 6 out of 7 DCISs showed an aberration of all three chromosomes simultaneously and one case showed an aberration of two chromosomes. All aberrations in case of DCIS were polysomic while two benign lesions and 15 IDCs showed a monosomic pattern.

Conclusion

FISH may enable more accurate diagnosis of intraductal breast lesions.

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Abbreviations

DCIS:

Ductal carcinomain situ

IDP:

Intraductal papilloma

FISH:

Fluorescence insitu hybridization

FNA:

Fine needle aspiration biopsy

LOH:

Loss of heterozygosity

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Authors and Affiliations

  1. Department of Surgery, Osaka Medical Center for Cancer andCardiovascular Diseases, Japan

    Yoshifumi Komoike, Kazuyoshi Motomura, Hideo Inaji & Hiroki Koyama

Authors
  1. Yoshifumi Komoike
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  2. Kazuyoshi Motomura
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  3. Hideo Inaji
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  4. Hiroki Koyama
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Komoike, Y., Motomura, K., Inaji, H. et al. Diagnosis of ductal carcinoma in situ (DCIS) and intraductal papilloma using fluorescence in Situ Hybridization (FISH) analysis. Breast Cancer 7, 332–336 (2000). https://doi.org/10.1007/BF02966400

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  • DOI: https://doi.org/10.1007/BF02966400

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