Abstract
Effect of plant growth regulators on morphogenesis was studied using callus derived from inflorescence segments of sugarcane cv. Co-91010 and CoC-671 cultured on MS medium supplemented with 2,4-D, malt extract, L-glutamine, casein hydroly sate and coconut water (CW). Comparison was made of different media combinations of CW (5,10%), kinetin (lmg/l), zeatin (0.5mg/l) andTDZ (0.5mg/l) to study callus growth and regeneration. Results showed that CW (5%) + zeatin (0.5mg/l) was more effective over other growth regulators for callus induction while CW 5% was found effective for the regeneration of healthy plants in cv. Co-91010. Different levels of proline and thioproline were tested on the induction of embryogenie callus and subsequent plant regeneration in var. CoC-671. Proline at 2.61M showed vigorous growth of embryogenie callus compared to thioproline. Plants regenerated were hardened and grown in the green house to maturity.
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Suprasanna, P., Choudhary, R.S., Desai, N.S. et al. Regulation of somatic embryogenesis by plant growth regulators in sugarcane. Sugar Tech 7, 123–128 (2005). https://doi.org/10.1007/BF02950596
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DOI: https://doi.org/10.1007/BF02950596