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Variation of ginkgolides and bilobalide contents in leaves and cell cultures ofGinkgo biloba L.

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Abstract

Ginkgolides (GK) and bilobalide are valuable compounds that belong to the lactone terpene. The contents of these metabolites were determined by HPLC from female and male tree ofGinkgo biloba L. The productivity ofG. biloba cells was also compared with the corresponding individual trees. High variations in the ginkgolides and bilobalide were observed from different individuals, plant parts, and cultured cells. The ginkgolides and bilobalide contents were different depending on the plant parts. Callus was obtained from various plant tissues, and NAA was better at callogenesis than 2,4-D in both the female and male trees. The plants and their corresponding cells showed considerable variation in their ginkgolides and bilobalide concentrations. The ginkgolides and bilobalide contents were not correlated with the production between dominant trees and their corresponding cells. Light irradiation enhanced the production of GK-A and GK-B, however, the concentration of bilobalide decreased under dark conditions.

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References

  1. Huh, H., E. J. Staba, and J. Singh (1992) Supercritical fluid chromatographic analysis of polyphenols inGinkgo biloba.J. Chromatogr. 600: 364–369.

    Article  CAS  Google Scholar 

  2. Kang, S. S., J. S. Kim, W. J. Kwak, and K. H. Kim (1990) Flavonoids from the leaves ofGinkgo biloba.Kor. J. Pharmacogn. 21: 111–120.

    CAS  Google Scholar 

  3. Kang, S. S., J. S. Kim, W. J. Kwak, and K. H. Kim (1990) Identification and quantitative analysis of flavanol glycosides fromGinkgo biloba leaves by high performance liquid chromatography.Kor. J. Pharmacogn. 21: 148–152.

    CAS  Google Scholar 

  4. Laurain, D., J. T. Guiller, J. C. Chenieux, and T. A. van Beek (1997) Production of ginkgolide and bilobalide in transformed and gametophyte derived cell cultures ofGinkgo biloba.Phytochemistry 46: 127–130.

    Article  CAS  Google Scholar 

  5. Carrier, D. J., T. A. van Beek, R. van der Heijden, and R. Veroorte (1998) Distribution of ginkgolides and terpenoids biosynthetic activity inGinkgo biloba.Phytochemistry 48: 89–92.

    Article  CAS  Google Scholar 

  6. Smith, P. F., K. Maclennan, and C. L. Darlington (1996) The neuroprotective properties of theGinkgo biloba leaf: a review of the possible relationship to platelet-activating factor (PAF).J. Ethnopharmacol. 50: 131–139.

    Article  CAS  Google Scholar 

  7. Son, Y. H. and H. W. Kim (1998) Above-ground biomass and nutrient distribution in a 15-year-old ginkgo (Ginkgo biloba) plantation in central Korea.Bioresource Technol. 63: 173–177.

    Article  CAS  Google Scholar 

  8. Kim, G. S., Y. W. Paek, K. M. Ko, S. J. Hwang, Y. J. Kim, S. J. Chung, and B. Hwang (1996) Detection of flavonoid compounds by cell culture ofGinkgo biloba L.Kor. J. Biotechnol Bioeng. 11: 1–7.

    Google Scholar 

  9. Misawa, M. and T. M. Nakanishio (1998) Antitumor compounds; Production by plant cell culture. pp. 199–207. In: Y. P. S. Bajaj (ed.).Biotechnology in Agriculture and Forestry. Vol. 4.Medicinal and Aromatic Plants. Springer-Verlag, Berlin, Germany.

    Google Scholar 

  10. Murashige, T. and F. Skoog (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture.Physiol. Plant. 15: 473–497.

    Article  CAS  Google Scholar 

  11. Huh, H. and J. Staba (1992) Ontogenic aspects of ginkgolide production inGinkgo biloba.Planta Med. 59: 232–239.

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Forestry, Kyungpook National University, 702-701, Daegu, Korea

    Young-Goo Park & Su-Jung Kim

  2. Department of Forest Science, Gyeongsang National University, 660-701, Jinju, Korea

    Hee-Young Jung, Young-Min Kang, Seung-Mi Kang & Myung-Suk Choi

  3. Department of Biotechnology, University of Agricultural Science, GKVK, 560065, Bangalore, India

    D. Theertha Prasad

  4. Department of Food Nutrition, Gyeongsang National University, 660-701, Jinju, Korea

    Sun-Won Kim

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  1. Young-Goo Park
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  2. Su-Jung Kim
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  3. Hee-Young Jung
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  4. Young-Min Kang
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  5. Seung-Mi Kang
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  6. D. Theertha Prasad
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  7. Sun-Won Kim
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  8. Myung-Suk Choi
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Correspondence to Myung-Suk Choi.

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Park, YG., Kim, SJ., Jung, HY. et al. Variation of ginkgolides and bilobalide contents in leaves and cell cultures ofGinkgo biloba L.. Biotechnol Bioproc E 9, 35–40 (2004). https://doi.org/10.1007/BF02949319

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  • DOI: https://doi.org/10.1007/BF02949319

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