Abstract
An effective and timesaving analytical method was developed for the determination of 12 ergot alkaloids (ergometrine, ergotamine, ergocristine, α-ergokryptine, ergosine, ergocornine, and their respective -inine isomers) in rye and rye flour. Samples were extracted with dichloromethane/ethyl acetate/methanol/aqueous ammonia (25%) (50/25/5/1, v/v/v/v), and extracts were purified using a basic alumina column. The eluate was dried in the nitrogen stream and redissolved in acetonitrile/ ammonia carbamate-buffer (0.2 g/1), (1/1, v/v), and injected into an HPLC-FLD system (λEx 330 nm, λEm 415 nm), using the same mixture as mobile phase and a Phenyl-Hexyl column. Detection limits for the individual compounds ranged from 0.01 μg/kg to 0.5 μg/kg. In sample material spiked with a mixture of these compounds at two different levels (13 μg/kg and 27 μg/kg per compound), mean (n=5) recoveries were at 101% (sr 6.4%) and 89% (sr 3.1%), respectively.
Similar content being viewed by others
References
Hofmann A (2000) Die Mutterkornalkaloide, Nachtschatten Verlag, Solothurn
European Food Safety Authority (2005) Opinion of the Scientific Panel on Contaminants in Food Chain on a request from the Commission related to ergot as undesirable substance in animal feed. EFSA J 225: 1–27
Klug C (1986) Bestimmung von Mutterkornalkaloiden in Lebensmitteln. MvP-Hefte, Max von Pettenkofer-Institut des Bundesgesundheitsamtes, Berlin
Wolff J, Neudecker C, Klug C, Weber R (1988) Chemische und toxikologische Untersuchungen über Mutterkorn in Mehl und Brot. Z Ernähr wiss 27: 1–22
Scott PM (in press) Ergot alkaloids - Analysis. In: Management of Mycotoxins in Food and Feeds for Improving Public Health. International Agency for Research in Cancer, Lyon, France
DIN (1994) DIN 32645 Chemische Analytik; Nachweis-, Erfassungs- und Bestimmungsgrenze; Ermittlung unter Wiederholbedingungen; Begriffe, Verfahren, Auswertung. Beuth Verlag, Berlin
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Müller, C., Klaffke, H.S., Krauthause, W. et al. Determination of ergot alkaloids in rye and rye flour. Mycotox Res 22, 197–200 (2006). https://doi.org/10.1007/BF02946741
Issue Date:
DOI: https://doi.org/10.1007/BF02946741