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Fermentation strategies for recombinant protein expression in the methylotrophic yeastPichia pastoris

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Abstract

Fermentation strategies for recombinant protein production inPichia pastoris have been investigated and are reviewed here. Characteristics of the expression system, such as phenotypes and carbon utilization, are summarized. Recently reported results such as growth model establishment, application of a methanol sensor, optimization of substrate feeding strategy, DOstat controller design, mixed feed technology, and perfusion and continuous culture are discussed in detail.

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  1. Biological Process Development Eacility, Department of Chemical Engineering, University of Nebraska-Lincoln, 68583-0919, Lincoln, Nebraska, USA

    Wenhui Zhang, Mehmet Inan & Michael M. Meagher

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  1. Wenhui Zhang
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Zhang, W., Inan, M. & Meagher, M.M. Fermentation strategies for recombinant protein expression in the methylotrophic yeastPichia pastoris . Biotechnol. Bioprocess Eng. 5, 275–287 (2000). https://doi.org/10.1007/BF02942184

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