Abstract
Soil column studies were used to evaluate petroleum hydrocarbon (PHC) remediation in soils from Kwajalein Atoll. Treatments included controls, and combinations of water, air, nutrients, and bioaugmentation with indigenous microbes (W, A, N, and M, respectively). Microbial colony forming units (CFU) decreased in the control columns and in treatments without air. Treatments including W + A + N and W + A + N + M exhibited increased CFU. One third of the PHC was removed by water and another third was removed by W + A + N and W + A + N + M treatments. Bioaugmentation with indigenous PHC degraders did not enhance bioremediation. Potential for bioremediation was demonstrated by air, water, and nutrient amendments.
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Bartha, R. and Atlas, R. M. (1987),Long-term environmental effects of offshore oil and gas development, Boesch, D. F., and Rabalais, N. N., eds., Elsevier, pp. 287–341.
Bartha, R. (1986),Microbial Ecol. 12, 155–172.
Leahy, J. G. and Colwell, R. R. (1990),Microbiol. Rev. 54, 305–315.
Thomas, J. M. and Ward, C. H. (1989),Environ. Sci. Technol. 23, 760–766.
Siegrist, R. L., Korte, N. E., Pickering, D. A., and Phelps, T. J. (1991),Bioremediation demonstration on Kwajalein Island: Site characterization and on-site biotreatability studies. ORNL/TM-11894, Martin Marietta Energy Systems, Inc., Oak Ridge, TN.
Phelps, T. J., Siegrist, R. L., Mackowski, R., and Pfiffner, S. M. (1992),Bioremediation of petroleum contaminated soil on Kwajalein Island: Microbiological characterization and biotreatability studies, Adler, H. I., Jolley, R. L., and Donaldson, T. L., eds., ORNL/TM-11925, pp. 37–42. Martin Marietta Energy Systems, Inc., Oak Ridge, TN.
Siegrist, R. L., Phelps, T. J., and Mackowski, R. (1992),Bioremediation of petroleum contaminated soil on Kwajalein Island: Microbiological characterization and biotreatability studies, Adler, H. I., Jolley, R. L., and Donaldson, T. L., eds., ORNL/TM-11925, pp. 52–69. Martin Marietta Energy Systems, Inc., Oak Ridge, TN.
Phelps, T. J. and Siegrist, R. L. (1993), ORNL/TM-12273, Martin Marietta Energy Systems, Inc., Oak Ridge, TN.
Phelps, T. J., Raione, E. G., White, D. C., and Fliermans, C. B. (1989),Geomicrobiology J. 7, 79–91.
Phelps, T. J., Ringelberg, D., Hedrick, D., Davis, J., Fliermans, C. B., and White, D. C. (1989),Geomicrobiology J. 6, 157–170.
Findlay, R. H., Pollard, P. C., and Moriarty, D. J. W. (1985),Can. J. Microbiol. 31, 493–498.
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The submitted manuscript has been authored by a contractor of the US Government under contract Mo. DE-AC05-84OR21400. Accordingly, the US Government reserves a nonexclusive royalty-free license to publish or reproduce the published form of this contribution or allow others to do so, for US Government purposes.
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Phelps, T.J., Siegrist, R.L., Korte, N.E. et al. Bioremediation of petroleum hydrocarbons in soil column lysimeters from Kwajalein Island. Appl Biochem Biotechnol 45, 835–845 (1994). https://doi.org/10.1007/BF02941853
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DOI: https://doi.org/10.1007/BF02941853