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Influence of cadmium on rubisco activation inCanavalia ensiformis L. leaves

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Abstract

We studied the effect of cadmium on chlorophylls and rubisco activation inCanavalia ensiformis L. leaves. Chlorophyll levels were reduced by 5.0 μM Cd. Rubisco activity at 5.0 μM Cd was significantly smaller than that at no treatment. Rubisco content showed patterns of change similar to rubisco activity. These data suggest that rubisco activity was associated with an amount of rubisco protein, and that the activation and induction of rubisco is inhibited by Cd. The degree of intensity of 50 and 14.5 kD polypeptides identified as the large and small subunit of rubisco by SDS-PAGE analysis at 5.0 μM Cd was significantly lower than that at control, indicating Cd had a effect on both subunits. Under the assumption that effects of Cd on rubisco may be related to rubisco activase, in addition to, its activity and content were determined. The rubisco activase activity at 5.0 μM Cd was more decreased than, the control. A similar change pattern was also observed in content of rubisco activase. Remarkable differences in the intensitiy of both the 45 kD and 41 kD band were found between at control and Cd-treatment. These results suggest that the change in the levels of rubisco activase leads to a subsequent alteration of rubisco levels.

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References

  1. Woodrow, I. E. and J. A. Berry (1988) Enzymatic regulation of photosynthetic CO2 fixation in C3 plants.Annu. Rev. Plant Physiol. Plant Mol. Biol. 39: 533–594.

    CAS  Google Scholar 

  2. Andrews, T. J. and G. H. Lorimer (1987) Rubisco: Structure, mechanisms, and prospects for improvements. pp. 131–218. In: M. D. Hatch, and N. K. Boardmaan (eds.),Biochemistry of Plants. Vol. 10, Academic Press, New York, USA.

    Google Scholar 

  3. Portis, A. R. Jr. (1990) Rubisco activase.Biochim. Biophys. Acta 1015: 15–28.

    Article  CAS  Google Scholar 

  4. Streusand, V. J. and Portis, A. R. Jr. (1987) Rubisco activase mediates ATP-dependent activation of ribulose bisphosphate carboxylase.Plant Physiol. 85: 152–154.

    Article  CAS  Google Scholar 

  5. Wang, Z. Y. and A. R. Portis, Jr. (1992) Dissociation of ribulose-1,5-bisphosphate carboxylase/oxygenase and its a enhancement by ribulose-1,5-bisphosphate carboxylase/oxygenase activase-mediated hydrolysis of ATP.Plant Physiol. 99: 1348–1353.

    Article  CAS  Google Scholar 

  6. Portis, A. R. Jr. (1992) Regulation of ribulose 1,5-bisphosphate carboxylase/oxygenase activity.Annu. Rev. Plant Physiol. Plant Mol. Biol. 43: 415–437.

    Article  CAS  Google Scholar 

  7. Moore, B. D. and J. R. Seemann (1994) Evidence that 2-C-carboxyarabinitol 1-phosphate binds to ribulose-1,5-bisphosphate carboxylasein vivo.Plant Physiol. 105: 731–737.

    CAS  Google Scholar 

  8. Zhu, G. and R. G. Jensen (1990) Status of the substrate binding sites of ribulose bisphosphate carboxylase as a determined with 2-C-carboxyarabinitol 1,5-bisphosphate.Plant Physiol. 93: 244–249.

    Article  CAS  Google Scholar 

  9. Prasad, M. N. V. (1995) Cadmium toxity and tolerance in vascular plants.Environ. Exp. Bot. 35: 525–545.

    Article  CAS  Google Scholar 

  10. Jackson, P. J., P. J. Unkefer, E. Delhaize, and N. J. Robinson (1990) Mechanism of trace metal tolerance in plants. pp. 231–255. In: F. Katterman (ed.)Environmental Injury to Plants. Academic Press, New York, USA.

    Google Scholar 

  11. Catalado, D. A., T. R. Garland, and R. E. Wildung (1981) Cadmium distribution and chemical fate in soybean plants.Plant Physiol. 68: 835–839.

    Article  Google Scholar 

  12. Vögeli-Lange, R. and G. J. Wagner (1990) Subcellular localization of cadmium and cadmium-binding peptides in tobacco leaves.Plant Physiol. 92: 1086–1093.

    Article  Google Scholar 

  13. Chen, Y. and A. J. Huerta (1997) Effects of sulfur nutrition on photosynthesis in cadmium-treated barley seedlings.J. Plant Nutrition 20: 845–856.

    Article  CAS  Google Scholar 

  14. Shah, K. and R. S. Dubey (1995) Effect of cadmium on RNA level as well as activity and molecular forms of ribonuclease in growing rice seedlings.Plant Physiol. Biochem. 33: 577–584.

    CAS  Google Scholar 

  15. Shaw, B. P. (1995) Effects of mercury and cadmium on the activities of antioxidative enzymes in the seedlings ofPhaseolus vulgaris.Biol. Plant. 37: 587–596.

    Article  CAS  Google Scholar 

  16. Panković, D., M. Plesničar, I. Arsenijević-Maksimović, N. Petrović, Z. Sakač, and R. Kastori (2000) Effects of nitrogen nutrition on phosynthesis in Cd-treated sunflower plants.Ann. Bot. 86: 841–847.

    Article  CAS  Google Scholar 

  17. Greger, M. and E. Ögren (1991) Direct and indirect effects of Cd2+ on photosynthesis in sugar beet (Beta vulgaris).Physiol. Plant. 83: 129–135.

    Article  CAS  Google Scholar 

  18. Siedlecka, A. and Z. Krupa (1996) Interaction between cadmium and iron and its effects on photosynthetic capacity of primary leaves ofPhaseolus vulgaris.Plant Physiol. Biochem. 34: 833–841.

    CAS  Google Scholar 

  19. Krupa, Z., G. Öquist, and N. P. A. Huner (1993) The effects of cadmium on photosynthesis ofPhaseolus vulgaris—a fluorescence analysis.Physiol. Plant. 88: 626–630.

    Article  CAS  Google Scholar 

  20. Roh, K. S., J. K. Kim, S. D. Song, H. S. Chung, and J. S. Song (1996) Decrease of the activation and carbamylation of rubisco by high CO2 in kidney bean.Kor. J. Biotechnol. Bioeng. 11: 295–302.

    Google Scholar 

  21. Roh, K. S., I. S. Kim, B. W. Kim, J. S. Song, H. S. Chung, and S. D. Song (1997) Decrease in carbamylation of rubisco by high CO2 concentration is due to decrease of rubisco activase in kidney bean.J. Plant Biol. 40: 73–79.

    Article  CAS  Google Scholar 

  22. Inskeep, W. P. and P. R. Bloom (1985) Extinction coefficients of chlorophylla andb inN,N-dimethylformamide and 80% acetone.Plant Physiol. 77: 483–485.

    Article  CAS  Google Scholar 

  23. Wang, Z. Y., G. W. Synder, B. D. Esau, A. R. Portis, Jr. and W. L. Ogren (1992) Species-dependent variation in the interaction of substrate-bound ribulose-1,5-bisphosphate carboxylase/oxygenase and rubisco activase.Plant Physiol. 100: 1858–1862.

    Article  CAS  Google Scholar 

  24. Racker, E. (1962) Ribulose diphosphate carboxylase from spinach leaves.Methods Enzymol. 5: 266–270.

    Article  CAS  Google Scholar 

  25. Robinson, S. P. and A. R. Portis, Jr. (1989) Adenosine triphosphate hydrolysis by purified rubisco activase.Arch. Biochem. Biophys. 268: 93–99.

    Article  CAS  Google Scholar 

  26. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of head of bacteriophage T4.Nature 227: 680–685.

    Article  CAS  Google Scholar 

  27. Weber, K. and M. Osborn (1969) The reliability of molecular weight determination by dodecyl sulfate-polyacrylamide gel electrophoresis.J. Biol. Chem. 244: 4406–4412.

    CAS  Google Scholar 

  28. Stobart, A. K., W. T. Griffiths, I. Ameen-Bukhari, and R. P. Sherwood (1985) The effect of Cd2− on the biosynthesis of chlorophyll in leaves of barley.Physiol. Plant. 63: 293–298.

    Article  CAS  Google Scholar 

  29. Bödddi, B., A. R. Oravecz, and E. Lehczki (1995) Effect of cadmium on organization and photoreduction of protochlorophyllide in dark-grown leaves and etioplast inner membrane preparations of wheat.Photosynthetica 31: 411–420.

    Google Scholar 

  30. Sandalio, L. M., M. C. Dalurzo, M. Gómez, M. C. Romero-Puertas, and L. A. del Río (2001) Cadmium-induced changes in the growth and oxidative metabolism of pea plants.J. Exp. Bot. 52: 2115–2126.

    CAS  Google Scholar 

  31. Dubé, S. L. and J. F. Bornman (1992) Response of spurce seedlings to simultaneous exposure to ultraviolet-B radiation and cadmium.Plant Physiol. Biochem. 30: 761–767.

    Google Scholar 

  32. Stiborova, M. (1988) Cd2+ ions affect the quaternary structure of ribulose-1.5 bisphosphate carboxylase from barley leaves.Biochemia Physiologia Pflanzen 183:371–378.

    CAS  Google Scholar 

  33. Stiborova, M., M. Ditrichova, and A. Brezinona (1987) Effect of heavy metal ions on growth and biochemical characteristics of photosynthesis of barley and maize seedlings.Biol. Plant. 29: 453–467.

    Article  CAS  Google Scholar 

  34. Siedlecka, A., Z. Krupa, G. Samuelson, G. Öquist, and P. Gardeströn (1997) Primary carbon metabolism inPhaseolus vulgaris plants under Cd/Fe interaction.Plant Physiol. Biochem. 35: 951–957.

    CAS  Google Scholar 

  35. Somerville, C. R., A. R. Portis, Jr., and W. L. Ogren (1982) A mutant ofArabidopsis thaliana which lacks activation of RuBP carboxylasein vivo.Plant Physiol. 70: 381–387.

    Article  CAS  Google Scholar 

  36. Woodrow, I. E. and K. A. Mott (1992) Biphasic activation of ribulose bisphosphate carboxylase in spinach leaves as determined from nonsteady-state CO2 exchange.Plant Physiol. 99: 298–303.

    Article  CAS  Google Scholar 

  37. Campbell, W. J. and W. L. Ogren (1990) Electron transport through photosystem I stimulates light activation of ribulose bisphosphate carboxylase/oxygenase (rubisco) by urbisco activase.Plant Physiol. 94: 479–484.

    Article  CAS  Google Scholar 

  38. To, K. Y., D. F. Suen, and S. C. G. Chen (1999) Molecular characterization of ribulose-1,5-bisphosphate carboxylase/oxygenase activase in rice leaves.Planta 209: 66–76.

    Article  CAS  Google Scholar 

  39. Salvucci, M. E., J. M. Werneke, W. L. Ogren, and A. R. Portis, Jr. (1987) Purification of species distribution of rubisco activase.Plant Physiol. 84: 930–936.

    Article  CAS  Google Scholar 

  40. Roh, K. S., E. J. Im, S. E. Yeo, M. J. Oh, J. S. Song, H. S. Chung, and S. D. Song (2001a) Exogenous GA3 increases rubisco activation in soybean leaves.J. Plant Biol. 44: 53–60.

    Article  CAS  Google Scholar 

  41. Roh, K. S., M. J. Oh, S. D. Song, H. S. Chung, and J. S. Song (2001b) Influence of benomyl on photosynthetic capacity in soybean leaves.Biotechnol. Bioprocess Eng. 6: 100–106.

    Article  CAS  Google Scholar 

  42. Roh, K. S., M. S. Kwan, Y. H. Do, J. S. Song, H. S. Chung, and S. D. Song (1998) Immunoblot analysis of the expression of genes for barley rubisco activase inE. coli.J. Plant Biol. 41: 233–239.

    Article  CAS  Google Scholar 

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  1. Department of Biology, Keimyung University, 704-701, Daegu, Korea

    Kyong Ro Lee & Kwang Soo Roh

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  1. Kyong Ro Lee
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Lee, K.R., Roh, K.S. Influence of cadmium on rubisco activation inCanavalia ensiformis L. leaves. Biotechnol Bioproc E 8, 94–100 (2003). https://doi.org/10.1007/BF02940263

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