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Growth kinetics and phenolics production inGlycine Max cell suspension cultures

Effect of microbial elicitor, calcium, polyamines, and organic osmolytes

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Abstract

Glycine max was used as a model plant cell suspension culture to establish relationships among growth kinetics, phenolics production, elicitor action, and peroxidase activity. Timing of elictor addition through monitoring of peroxidase provided an excellent means of optimizing yields of phenolics and reduced the time span during which phenolics were formed, negating the need for a secondary production medium. We have also determined that calcium and other cellular effectors like polyamines and organic osmolytes, when used in conjunction with elicitors, enhance phenolics production. Calcium directly enhanced elicitation, whereas polyamines and other osmolytes such as glycerol and proline extended cell viability. The study also demonstrated potential for enhancing secondary metabolite production by a combination of elicitation, cell viability stabilizers, and by addition of nutrients at the time of elicitation.

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Authors and Affiliations

  1. Department of Bacteriology and Biochemistry, Moscow

    Kalidas Shetty & Dom L. Crawford

  2. Department of Chemical Engineering, University of Idaho, 83843, Moscow, ID

    roger A. Korus

Authors
  1. Kalidas Shetty
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  2. roger A. Korus
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  3. Dom L. Crawford
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Shetty, K., Korus, r.A. & Crawford, D.L. Growth kinetics and phenolics production inGlycine Max cell suspension cultures. Appl Biochem Biotechnol 20, 825–843 (1989). https://doi.org/10.1007/BF02936529

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