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Induction of nonspecific cross-reacting antigen mRNA by interferon-γ and anti-fibronectin receptor antibody in colon cancer cells

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Abstract

Nonspecific cross-reacting antigen (NCA), a member of the carcinoembryonic antigen (CEA) family, shows increased expression levels in colorectal cancer tissues. To elucidate the mechanism, we observed the effect of interon (IFN)-γ on the expression level of NCA mRNA in colon cancer cell lines by quantitative reverse transcriptase-polymerase chain reaction assay. IFN-γ induced NCA mRNA in three of four cell lines tested. The effect of anti-fibronectin receptor (FnR) antibody on the expression of NCA mRNA was then examined in the same manner. Colo201 and DLD-1 cells showed an increased expression level of NCA mRNA after stimulation with the antibody. On flow cytometry, FnR was expressed in only two, Colo201 and DLD-1, of the five cell lines tested. These findicate that IFN-γ and anti-FnR antibody induce NCA mRNA in cultured colon cancer cell lines, suggesting that inflammatory response and cell-to-extracellular matrix interaction may be related to the increased expression of NCA mRNA in colorectal cancers in vivo.

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Authors and Affiliations

  1. First Department of Internal Medicine, Sapporo Medical University, S-1, W-16, Chuo-ku, 060, Sapporo, Japan

    Yuji Hinoda, Tehzoh Saito, Hiroki Takahashi, Fumio Itoh, Masaaki Adachi & Kohzoh Imai

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  1. Yuji Hinoda
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  2. Tehzoh Saito
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  3. Hiroki Takahashi
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  4. Fumio Itoh
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  5. Masaaki Adachi
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  6. Kohzoh Imai
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Hinoda, Y., Saito, T., Takahashi, H. et al. Induction of nonspecific cross-reacting antigen mRNA by interferon-γ and anti-fibronectin receptor antibody in colon cancer cells. J Gastroenterol 32, 200–205 (1997). https://doi.org/10.1007/BF02936368

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  • DOI: https://doi.org/10.1007/BF02936368

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