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Calcitonin inhibits prolactin gene transcription in rat pituitary cells

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Abstract

Our recent studies have shown that calcitonin (CT)-like immunoreactive peptide is synthesized and released from cultured rat anterior pituitary (AP) cells, and may serve as a paracrine inhibitor of PRL release. The present studies investigated effects of CT on basal and TRH-induced PRL mRNA levels in rat AP and rat pituitary tumor GH3 cells. CT attenuated steady-state PRL mRNA levels in a dose-dependent fashion in primary rat AP and GH3 cells. The kinetics of CT action suggests that 100nm CT caused a significant decline after 3 h, and the inhibition was sustained at least until the longest tested incubation period of 30 h. Results from nuclear run-on assays suggest that 100 nM CT decreased the rate of PRL gene transcription by 80% after 30 min of incubation. CT did not affect PRL mRNA levels in Ca2+-depleted GH3 cells but dramatically decreased them in Ca2+-repleted cells. Bay K 8644 induced increase in PRL mRNA levels of Ca2+-repleted GH3 cells and CT did not affect this increase. These results suggest that CT rapidly and selectively inhibits PRL gene transcription in primary AP and GH3 cells, and support a possibility that CT-induced attenuation of PRL mRNA may involve cytoplasmic Ca2+.

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References

  • Abe, H., Engler, D., Molitch, M., Bolinger-Gruber, J. & Reichlin, S. (1985).Endocrinology,116, 1383–1390.

    PubMed  CAS  Google Scholar 

  • Albrandt, K., Mull, E., Brady Elize, M., Herich, J., Moore, C.X. & Beaumont, K. (1993).FEBS Lett.,325, 225–232.

    Article  PubMed  CAS  Google Scholar 

  • Arita, N., Yasuhiro, K. & Kimura, F. (1992).Endocrinology,130, 3167–3174.

    Article  PubMed  CAS  Google Scholar 

  • Arnaout, M.A., Gartwaite, T.L., Martinson, D.R. & Hagen, T.C. (1986).Endocrinol,119, 2052–2057.

    CAS  Google Scholar 

  • Bancroft, S.C. (1981). Sato G. (ed):Functionally differentiated cell lines. Alan R. Liss: New York, pp 47–59.

    Google Scholar 

  • Bandyopadhyay, S.K. & Bancroft, C. (1989).J. Biol. Chem.,264, 14216–14219.

    PubMed  CAS  Google Scholar 

  • Blum, M. (1989).Methods in Enzymol.,168, 618–652.

    Article  CAS  Google Scholar 

  • Boockfor, F.R. & Frawley, L.S. (1987).Endocrinology,120, 874–879.

    Article  PubMed  CAS  Google Scholar 

  • Chomczynisky, P. & Sacchi, N. (1987).Anal Biochem.,162, 156–159.

    Google Scholar 

  • Davis, L.G., Dibner, M.D. & Battey, J.F. (1986).Basic Methods in Molecular Biology. Elsevier: New York, NY, p. 143.

    Google Scholar 

  • Davis, L.G., Dibner, M.D. & Battey, J.F. (1986).Basic Methods in Molecular Biology. Elsevier: New York, NY. p. 147.

    Google Scholar 

  • Day, R.N. & Maurer, R.A. (1990).Mol. Endocrinol.,4, 736–742.

    PubMed  CAS  Google Scholar 

  • Delidow, B.C., Lail-Trecker, M. & White, B.A. (1992).Mol Endocrinol,6, 1268–1276.

    Article  PubMed  CAS  Google Scholar 

  • Deschepper, C.F., Crumrine, D.A. & Ganong, W.F. (1986).Endocrinology,119, 36–43.

    PubMed  CAS  Google Scholar 

  • Elsholz, H.P., Lew, A.M., Albert, P.R. & Sundmark, V.C. (1991).J. Biol. Chem.,266, 22919–22925.

    Google Scholar 

  • Enyeart, J.J., Biangi, B. & Day, R.N. (1990).Mol Endocrinol,4, 727–735.

    PubMed  CAS  Google Scholar 

  • Fischer, J.A., Tobler, P.H., Henke, H. & Tschopp, F.A. (1983).J. Clin. Endocrinol. Metab.,57, 1314–1315.

    Article  PubMed  CAS  Google Scholar 

  • Groppe, C., Luster, W. & Havemann, K. (1985).Br. J. Cancer,51, 897–901.

    Google Scholar 

  • Henke, H., Tobler, P.H. & Fischer, J.A. (1983).Brain Res.,272, 373–378.

    Article  PubMed  CAS  Google Scholar 

  • Isaac, R., Merceron, R., Caillens, G., Raymond, J-P. & Ardaillou, R. (1980).J. Clin. Endocrinol. Metab,50, 1011–1015.

    PubMed  CAS  Google Scholar 

  • Judd, A.M., Kubota, T., Kuan, S.I., Jarvis, W.D., Spangelo, B.L. & MacLeod, R.M. (1990).Endocrinology,127, 191–199.

    PubMed  CAS  Google Scholar 

  • Kaplan, L.M., Gabriel, S.M., Koenig, J.I., Sunday, M.E., Spindel, E.R., Martin, J.B. & Chin, W.W. (1988).Proc. Natl. Acad. Sci. USA,85, 7408–7412.

    Article  PubMed  CAS  Google Scholar 

  • Kazemzadeh, M., Velkeniers, B., Herregodts, P., Collumbien, R., Finne, E., Derde, M.P., Vanhaelst, L. & Hooghe-Peters, E.L. (1992).J. Endocr.,132, 401–409.

    PubMed  CAS  Google Scholar 

  • Kudlow, J.E. & Kobrin, M.S. (1984).Endocrinology,115, 911–917.

    PubMed  CAS  Google Scholar 

  • Lamberts, S.W.J. & Macleod, R.M. (1990).Physiol Rev.,70, 270–318.

    Google Scholar 

  • Lamberts, S.W., Zuyderwijk, J., Den Holder, F., Van Koestveld, P. & Hofland, L.J. (1989).Neuroendocrinology,50, 44–50.

    Article  PubMed  CAS  Google Scholar 

  • Lasmoles, F., Julliene, A., Day, F., Minvielle, S., Mihaud, G. & Moukhtar, M.S. (1985).EMBO J.,4, 2603–2607.

    PubMed  CAS  Google Scholar 

  • Maurer, R.A. (1981).Nature,294, 94–97.

    Article  PubMed  CAS  Google Scholar 

  • Maurer, R.A. (1982).J. Biol. Chem.,257, 2133–2136.

    PubMed  CAS  Google Scholar 

  • Maurer, R., Marbach, P. & Mousson, R. (1983).Brain Res. 272, 346–348.

    Article  Google Scholar 

  • Murdoch, G.H., Franco, R., Evans, R.M. & Rosenfeld, M.G. (1983).J. Biol. Chem.,258, 15329–15335.

    PubMed  CAS  Google Scholar 

  • Potter, E., Nicolaisen, A.K., Ong, E.S., Evans, R.M. & Rosenfeld, M.G. (1981).Proc. Natl. Acad. Sci. USA,78, 6662–6666.

    Article  PubMed  CAS  Google Scholar 

  • Preston, G.M., Billis, W.M., Agarwal, P. & White, B.A. (1990).Mol. Cell. Biol.,10, 442–448.

    PubMed  CAS  Google Scholar 

  • Pun, K.K., Varghese, Z. & Moorhead, J.F. (1987).Acta Endocrinol.,115, 243–246.

    PubMed  CAS  Google Scholar 

  • Shah, G.V., Deftos, L.J. & Crowley, W.R. (1992).Endocrinology,132, 1367–1372.

    Article  Google Scholar 

  • Shah, G.V., Epand, R.M. & Orlowsky, R.C. (1988).J. Endocrinol,116, 279–286.

    PubMed  CAS  Google Scholar 

  • Shah, G.V., Kacsoh, B., Seshadri, R., Grosvenor, C.E. & Crowley, W.R. (1989).Endocrinology,125, 61–67.

    PubMed  CAS  Google Scholar 

  • Shah, G.V., Kennedy, D., Dockter, M.E. & Crowley, W.R. (1990a).Endocrinology,127, 613–620.

    PubMed  CAS  Google Scholar 

  • Shah, G.V., Wang, W., Grosvenor, C.E. & Crowley, W.R. (1990b).Endocrinology,127, 621–628.

    PubMed  CAS  Google Scholar 

  • Tashjian Jr, A.H. (1979).Methods Enzymol,58, 527–535.

    Article  PubMed  Google Scholar 

  • Velkeniers, B., Hooghe-Peter, E.L., Hooghe, R., Belayew, A., Smets, S., Clayes, A., Robberrecht, P. & Vanhaelst, L. (1988).Endocrinology,123, 1619–1630.

    PubMed  CAS  Google Scholar 

  • White, B.A. & Bancroft, C. (1987).Methods Enzymol,139, 655–666.

    Article  PubMed  CAS  Google Scholar 

  • Xie, W.Q. & Rothblum, L.I. (1991).Biotechniques,11, 325–327.

    Google Scholar 

  • Yan, G. & Bancroft, C. (1991).Mol. Endocrinol.,5, 1488–1497.

    PubMed  CAS  Google Scholar 

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Author notes

  1. Qing Xue-Zhang

    Present address: Department of Cardiothoracic Surgery, Cornell University Medical College, P. O. Box 378, 10021, New York, NY

Authors and Affiliations

  1. Department of Urologic Surgery, University of Kansas Medical Center, 66160, Kansas City, Kansas, USA

    Qing Xue-Zhang, Scott M. Stanley & Girish V. Shah

  2. Department of Physiology, University of Kansas Medical Center, 66160, Kansas City, Kansas, USA

    Qing Xue-Zhang, Scott M. Stanley & Girish V. Shah

Authors
  1. Qing Xue-Zhang
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  2. Scott M. Stanley
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  3. Girish V. Shah
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Xue-Zhang, Q., Stanley, S.M. & Shah, G.V. Calcitonin inhibits prolactin gene transcription in rat pituitary cells. Endocr 3, 445–451 (1995). https://doi.org/10.1007/BF02935651

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