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Non-invasive methods for determination of cellular growth inPodophyllum hexandrum suspension cultures

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Abstract

Culture conductivity and on-line NADH fluorescence were used to measure cellular growth in plant cell suspension cultures ofPodophyllum hexandrum. An inverse correlation between dry cell weight and medium conductivity was observed during shake flask cultivation. A linear relationship between dry cell weight and culture NADH fluorescence was obtained during the exponential phase of batch cultivation in a bioreactor under the pH stat (pH 6) conditions. It was observed that conductivity measurement were suitable for biomass characterisation under highly dynamic uncontrolled shake flask cultivation conditions. However, if the acid/alkali feeding is done for pH control the conductivity measurement could not be applied. On the other hand the NADH fluorescence measurement allowed online-in situ biomass monitoring of rather heterogenous plant cell suspension cultures in bioreactor even under the most desirable pH stat conditions.

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Authors and Affiliations

  1. Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, 110016, New Delhi, India

    Saurabh Chattopadhyay, V. S. Bisaria & A. K. Srivastava

  2. Institut fuer Technische Chemie, Callinstrasse 3, 30167, Hannover, Germany

    T. Scheper

Authors
  1. Saurabh Chattopadhyay
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  2. V. S. Bisaria
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  3. T. Scheper
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  4. A. K. Srivastava
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Correspondence to A. K. Srivastava.

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Chattopadhyay, S., Bisaria, V.S., Scheper, T. et al. Non-invasive methods for determination of cellular growth inPodophyllum hexandrum suspension cultures. Biotechnol. Bioprocess Eng. 7, 331–334 (2002). https://doi.org/10.1007/BF02933516

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  • DOI: https://doi.org/10.1007/BF02933516

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