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The effects of agitation and aeration on the production of gluconic acid byAspergillus niger

  • Session 3 Bioprocessing Research
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Abstract

The effects of agitation and aeration in the production of gluconic acid byAspergillus niger from a glucose medium were investigated. Experiments were conducted at aeration rates of 5.0 and 10.0 L/min. Four different agitation speeds were investigated for each aeration rate. Gluconic acid concentration and biomass concentration were analyzed, and the rate of consumption of substrate byA. niger was noted. The main purpose of this work was to find the optimal conditions of agitation and aeration for the growth ofA. niger and production of gluconic acid in submerged culture in a batch fermentor at a bench-top scale. The oxygen-transfer rates at different agitation and aeration rates were calculated. The gluconic acid concentration and rate of growth ofa. niger increased with increase in the agitation and aeration rates.

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Abbreviations

N :

oxygen uptake rate, mmol/L/min

y :

yield, g dry cells produced/100 g glucose used

U :

growth rate, g dry cells/L/min

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Authors and Affiliations

  1. Department of Chemical Engineering, University of Louisville, 40292, Louisville, KY

    Sundeep N. Dronawat, C. Kurt Svihla & Thomas R. Hanley

Authors
  1. Sundeep N. Dronawat
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  2. C. Kurt Svihla
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  3. Thomas R. Hanley
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Dronawat, S.N., Svihla, C.K. & Hanley, T.R. The effects of agitation and aeration on the production of gluconic acid byAspergillus niger . Appl Biochem Biotechnol 51, 347–354 (1995). https://doi.org/10.1007/BF02933438

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  • DOI: https://doi.org/10.1007/BF02933438

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