Abstract
Glycerol production from xylose fermentation usingRhizopus javanicus (ATCC 22581) has been investigated in shake flasks. The medium composition (xylose concentration, nitrogen sources), aeration rate, and temperature have been found to affect the accumulation and yield of glycerol. Some of these effects are explained in terms of the critical parameters, osmotic pressure, and dissolved oxygen levels in the medium. Relatively high glycerol yields and concentrations have been obtained at high sugar concentration with high level of aeration at room temperature. The addition of polyethylene glycol or sulfite can improve the yield and accumulation of glycerol.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Miner, C. S. and Dalton, N. N., eds. (1953),Glycerol, Rheinhold, New York.
Newman, A. A. (1968), inGlycerol, CRC, Cleveland, pp. 1–166.
Jeffries, T. W. (1985),Biotechnol. Bioeng. Symp. 15, 150–166.
Pettersen, R. C. (1984), inThe Chemistry of Solid Wood, Rowell, R. M., ed.,Advances in Chemistry Series, vol. 207, American Chemical Society, Washington, DC, pp. 57–126.
Spencer, J. F. T. (1968), inProgress in Industrial Microbiology, Hockenhall, D. J. D., ed.,7, 1–42.
Parekh, S. R. and Pandey, N. K. (1985),Biotechnol. Bioeng. 27, 1089–1091.
Hecker, D., Bisping, B., and Rehm, H.-J. (1990),Appl. Microbiol. Biotechnol. 32, 627–632.
Vijaikishore, P. and Karanth, N. G. (1984),Appl. Biochem. Biotechnol. 9, 243–253.
Brown, A. D. and Simpon, J. R. (1972),J. Gen. Microbiol. 72, 589–591.
Hahn-Hägerdal, B., Jönsson, B., and Lohmeier-Vogal, E. (1985),Appl. Microbiol. Biotechnol. 21, 173–175.
Esener, A. A., Bol, G., Kossen, N. W. F., and Roels, J. A. (1981), inAdvances in Biotechnology, vol. 1, Moo-Young, M., Robinson, C. W., and Vezina, C., eds., Pergamon, Toronto, p. 339.
Connstein, W. and Lüdecke, K. (1921), US Patent 1,368,023.
Connstein, W. and Lüdecke, K. (1924), US Patent, 1,511,754.
Underkofler, L. A. and Hickey, R. J., eds. (1954),Industrial Fermentations, vol. 1, Chemical Publishing Company, p. 252.
Neuberg, C., Hirsch, J., and Reinfurth, E. (1920),Biochem. Z. 105, 307.
Bisping, B. and Rehm, H.-J. (1982),Eur. J. Appl. Microbiol. Biotechnol. 14, 136–139.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lu, Z., Yang, CW. & Tsao, G.T. Fermentation of xylose to glycerol byRhizopus javanicus . Appl Biochem Biotechnol 51, 83–95 (1995). https://doi.org/10.1007/BF02933413
Issue Date:
DOI: https://doi.org/10.1007/BF02933413