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Effects of three-dimensional scaffolds on cell organization and tissue development

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Abstract

Tissue engineering scaffolds play a critical role in regulating the reconstructed human tissue development. Various types of scaffolds have been developed in recent years, including fibrous matrix and foam-like scaffolds. The design of scaffold materials has been investigated extensively. However, the design of physical structure of the scaffold, especially fibrous matrices, has not received much attention. This paper compares the different characteristics of fibrous and foam-like scaffolds, and reviews regulatory roles of important scaffold properties, including surface geometry, scaffold configuration, pore structure, mechanical property and bioactivity. Tissue regeneration, cell organization, proliferation and differentiation under different microstructures were evaluated. The importance of proper scaffold selection and design is further discussed with the examples of bone tissue engineering and stem cell tissue engineering. This review addresses the importance of scaffold microstructure and provides insights in designing appropriate scaffold structure for different applications of tissue engineering.

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Abbreviations

ALP:

alkaline phosphatase

BMP:

bone morphogenetic protein

ECM:

extracellular matrix

EGF:

epidermal growth factor

GAG:

glycosaminoglycans

PEG:

poly(ethylene glycol)

PET:

polyethylene terephthalate

PGA:

poly(glycolic acid)

PLA:

poly(lactic acid)

PGLA:

poly(glycolic-co-lactic acid)

PLG:

poly(lactic-co-glycolide)

PLGA:

poly(lactic-co-glycolic acid)

PTFE:

polytetrafluoroethylene

VEGF:

vascular endothelial growth factor

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  1. Department of Chemical Engineering, The Ohio State University, 43210, Columbus, OH, USA

    Yan Li & Shang-Tian Yang

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Li, Y., Yang, ST. Effects of three-dimensional scaffolds on cell organization and tissue development. Biotechnol. Bioprocess Eng. 6, 311–325 (2001). https://doi.org/10.1007/BF02932999

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