Skip to main content
Log in

Development of embryos and seedlings from pollen grains inLycium halimifolium Mill. in thein vitro culture

  • Brief Communication
  • Published:
Biologia Plantarum

Abstract

Anthers ofLycium halimifolium were grownin vitro at the following stages of development: tetrads, microspores and binucleate pollen grains. Pollen plantlets were obtained only from anthers inoculated at the stage of microspores. The growth of androgenic embryos was very slow. Mature embryos were formed in about 3% of the inoculated anthers. The largest number of embryos obtained from one anther was 4.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Clapham, D.:In vitro development of callus from the pollen ofLolium andHordeum. — Z. Pflanzenzücht.65: 285–292, 1971.

    Google Scholar 

  • Guha, S., Maheshwari, S. C.: Cell division and differentiation of embryos in the pollen grains ofDatura in vitro, — Nature212: 97–98, 1966.

    Article  Google Scholar 

  • Guha, S., Iyer, R. D., Gupta, N., Swaminathan, M. S.: Totipotency of gametic cells and the production of haploids in rice. — Curr. Sci.39: 174–176, 1970.

    Google Scholar 

  • Linsmaieb, E. M., Skoog, F.: Organic growth factor requirements of tobacco tissue cultures. — Physiol. Plant.18: 100–127, 1965.

    Article  Google Scholar 

  • Kameya, T., Hinata, K.: Induction of haploid plants from pollen grains ofBrassica. — Jap. J. Breed.20: 82–87, 1970.

    Article  Google Scholar 

  • Nakata, K., Tanaka, M.: Differentiation of embryoids from developing germ cells in anther culture of tobacco. — Jap. J. Genet.43: 65–71, 1968.

    Article  Google Scholar 

  • Narayanaswamy, S., Chandy, L. P.:In vitro induction of haploid, and triploid androgenic embryoids and plantlets inDatura metel L. — Ann. Bot.35: 535–542, 1971.

    Google Scholar 

  • Niizeki, H., Oono, K.: Induction of haploid rice plant from anther culture. — Proc. Jap. Acad.44: 554–557, 1968.

    Google Scholar 

  • Nitsch, J. P.: Experimental androgenesis inNicotiana. — Phytomorphology19: 389–404, 1969.

    Google Scholar 

  • Rangaswamy, N. S.: Experimental studies on female reproductive structures ofCitrus microcarpa Bunge. — Phytomorphology11: 109–127, 1961.

    Google Scholar 

  • Sunderland, N., Wicks, F. M.: Cultivation of haploid plants from tobacco pollen. — Nature224: 1227–1229, 1969.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zenkteler, M. Development of embryos and seedlings from pollen grains inLycium halimifolium Mill. in thein vitro culture. Biol Plant 14, 420–422 (1972). https://doi.org/10.1007/BF02932983

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02932983

Keywords

Navigation