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Concanavalin A distribution in polymorphonuclear leukocytes and alveolar macrophages during hyperoxia

  • Published:
Virchows Archiv B

Summary

Hyperoxia impaires cytoskeleton-dependent phagocytic functions in polymorphonuclear leukocytes (PMNs) and alveolar macrophages (AMs). To investigate the effect of different oxygen concentrations on the cytoskeleton, in particular the microtubule (MT) and microfilament (MF) system, the fluorescence pattern of Concanavalin A (Con A) receptors in AMs and PMNs was observed. Cells were obtained from guinea-pigs exposed to different oxygen concentrations. The exposure of guinea-pigs to oxygen concentrations of up to 50% induced in AMs and PMNs mainly spontaneous Con A capping, demonstrating an altered MT system. Oxygen tensions above 50% lead to an increased number of AMs and PMNs exhibiting a patchy Con A fluorescence distribution. Even in the presence of the microtubule-disrupting agent colchicine most AMs and PMNs were unable to form a Con A cap fluorescence distribution under these high oxygen tensions. This study demonstrates that the exposure of guinea-pigs to an oxygen concentration greater than 50% increases the relative number of AMs and PMNs demonstrating a patchy distribution of Con A. This patchy fluorescence pattern is associated with a severe cytoskeletal defect, i.e. MT and MF disruption. The various leukocyte function defects induced by hyperoxia, demonstrated in previous studies, are based on this MT and MF alteration in AMs and PMNs, representing an additional sepsis-promoting factor during hyperoxia.

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Abbreviations

PMNs:

polymorphonuclear leukocytes

AMs:

alveolar macrophages

MTs:

microtubules

MFs:

microfilaments Con A deConcanavalin A

KRP-buffer:

Krebs-Ringer-Phosphate-buffer

FiO2 :

fraction of inspired oxygen

GSH:

reduced glutathione

SSG:

oxidized glutathione

References

  • Autor AP, Frank L, Roberts JR (1976) Developmental characteristics of pulmonary Superoxide dismutase. Pediat Res 10:154–158

    Article  PubMed  CAS  Google Scholar 

  • Baehner RL, Boxer LA, Allen JM, Davies J (1977) Autooxidation as a basis for altered functions of polymorphonuclear leukocytes. Blood 50:327–335

    PubMed  CAS  Google Scholar 

  • Berlin RD, Oliver JM, Ukena TE, Yin HH (1975) The cell surface. New England J Med 292:515–520

    CAS  Google Scholar 

  • Berlin RD, Oliver JM (1978) Analogous ultrastructure and surface properties during capping and phagocytosis in leukocytes. J Cell Biol 77:789–804

    Article  PubMed  CAS  Google Scholar 

  • Bowden DH, Adamson IYR (1974) Endothelial regeneration as a marker of the differential vascular responses in oxygen induced edema. Lab Invest 30:350–357

    PubMed  CAS  Google Scholar 

  • Boxer LA, Watanabe AM, Rister M, Besch HR, Allen J, Baehner RL (1976) Correction of leukocyte function in Chediak-Higashi syndrome by ascorbate. New England J Med 296:1041–1045

    Google Scholar 

  • Boxer LA, Rister M, Allen J, Baehner RL (1977) Improvement of Chediak-Higashi leukocyte function by cyclic guanosine monophosphate. Blood 49:9–17

    PubMed  CAS  Google Scholar 

  • Boxer LA, Allen JN, Watanabe AM, Besch HR, Baehner RL (1978) Role of microtubules in granulocyte adherence. Blood 51:1045–1050

    PubMed  CAS  Google Scholar 

  • Burchill BR, Oliver JM, Pearson CB, Leinbach RD, Berlin RD (1978) Microtubule dynamics and glutathione metabolism in phagocytizing human polymorphonuclear leukocytes. J Cell Biol 76:439–447

    Article  PubMed  CAS  Google Scholar 

  • Clark JM (1974) The toxicity of oxygen. Am Rev Resp Dis 110:40–50

    PubMed  CAS  Google Scholar 

  • Crapo JD, Tierney DF (1974) Superoxide dismutase and pulmonary oxygen toxicity. Am J Physiol 226:1401–1407

    PubMed  CAS  Google Scholar 

  • Fischer AD, Diamond S, Meilen S (1974) Effects of O2 exposure on metabolism of the rabbit alveolar macrophage. J Appl Physiol 37:341–345

    Google Scholar 

  • Fridovich I (1970) Quantitative aspects of the production of Superoxide anion radical by milk xanthine oxidase. J Biol Chem 245:4053–4057

    PubMed  CAS  Google Scholar 

  • Hoffstein S, Soberman R, Goldstein I, Weissmann G (1976) Concanavalin A induces microtubule assembly and specific granule discharge in human polymorphonuclear leukocytes. J Cell Biol 68:781–787

    Article  PubMed  CAS  Google Scholar 

  • Huber GL, La Force M, Manson R (1970a) Impairment and recovery of pulmonary antibacterial defense mechanisms after oxygen administration. J Clin Invest 49:47a

    Google Scholar 

  • Huber GL, La Force MF (1970b) Progressive impairment of pulmonary antibacterial defense mechanism associated with prolonged oxygen administration. Ann Int Med 72:808a

    Google Scholar 

  • Huber GL, Goodenough S, O’Connell D (1974) Oxygen toxicity and bacterial infection in the lung. Clin Res 22:506a

    Google Scholar 

  • Karnovsky M (1962) Metabolic basis of phagocytic activity. Physiol Rev 42:143–167

    PubMed  CAS  Google Scholar 

  • Lehrer RI (1973) Effects of Colchicine and Chloramphenicol on the oxidative metabolism and phagocytic activity of human neutrophils. J Infect Dis 127:40–48

    PubMed  CAS  Google Scholar 

  • Mohri H (1976) The function of tubulin in motile systems. Biochem Biophys Acta 456:85–127

    PubMed  CAS  Google Scholar 

  • Murphey SA, Hyams JS, Fischer AB, Root RK (1975) Effects of oxygen exposure on in vitro function of pulmonary alveolar macrophages. J Clin Invest 56:503–511

    Article  PubMed  CAS  Google Scholar 

  • Nicolson GL, Poste G (1976) The cancer cell: Dynamic aspects and modifications in cell surface organization. New England J Med 195:197–203

    Google Scholar 

  • Northway WH, Rezau L, Petriceks R, Besch K (1976) Oxygen toxicity in the newborn lung. Reversal of inhibition of DNA synthesis in the mouse. Pediatrics 57:41–46

    PubMed  CAS  Google Scholar 

  • Oliver JM, Ukena TE, Berlin RD (1974) Effects of phagocytosis and Colchicine on the distribution of lectinbinding sites on cell surfaces. Proc Natl Acad Sci USA 71:394–398

    Article  PubMed  CAS  Google Scholar 

  • Oliver JM, Zurier RB, Berlin RD (1975a) Concanavalin A cap formation on polymorphonuclear leukocytes of normal and beige (Chediak-Hiagashi) mice. Nature 253:471–473

    Article  PubMed  CAS  Google Scholar 

  • Oliver JM (1975b) Microtubules, cyclic GMP and control of cell surface topography. Immune recognition. Academic Press, New York, pp 445–471.

    Google Scholar 

  • Oliver JM, Albertini DF, Berlin RD (1976a) Effects of glutathione oxidizing agents on microtubule assembly and microtubule-dependent surface properties of human neutrophils. J Cell Biol 71:921–932

    Article  PubMed  CAS  Google Scholar 

  • Oliver JM, Zurier RB (1976b) Correction of characteristic abnormalities of microtubule function and granule morphology in Chediak-Higashi syndrome. J Clin Invest 57:1239–1247

    Article  PubMed  CAS  Google Scholar 

  • Oliver JM (1976c) Impaired microtubule function correctable by cyclic GMP and cholinergic agonists in the Chediak-Higashi syndrome. Am J Pathol 85:395–418

    PubMed  CAS  Google Scholar 

  • Oliver JM, Berlin RD, Baehner RL, Boxer LA (1977) Mechanism of microtubule disassembly in vivo: Studies in normal and chronic granulomatous disease leukocytes. Br J Haem 37:311–322

    Article  CAS  Google Scholar 

  • Oliver JM Spielberg SP, Pearson CB, Schulman JD (1978) Microtubule assembly and function in normal and glutathione synthetase deficient polymorphonuclear leukocytes. J Immunol 120:1181–1186

    PubMed  CAS  Google Scholar 

  • Oren R, Farhan AE, Saito K, Mikofsky E, Karnovsky ML (1963) Metabolic patterns in three types of phagocytizing cells. J Cell Biol 17:487–501

    Article  PubMed  CAS  Google Scholar 

  • Poste G, Papahdiopopulos D, Jacobson K, Vail WJ (1975) Effects of local anesthetics on membrane properties. Biochem Biophys Acta 394:520–539

    Article  PubMed  CAS  Google Scholar 

  • Pratt PC (1974) Pathology of pulmonary oxygen toxicity. Am Rev Resp Dis110:51–57

    PubMed  CAS  Google Scholar 

  • Resnick JS, Brown DM, Wenier RL (1974) Oxygen toxicity in fetal organ culture. II The developing lung. Lab Invest 31:665–677

    CAS  Google Scholar 

  • Rister M, Baehner RL (1976) The alteration of Superoxide dismutase, catalase, glutathione peroxidase, and NAD(P)H cytochrome C reductase in guniea-pig polymorphonuclear leukocytes and alveolar macrophages during hyperoxia. J Clin Invest 58:1174–1184

    Article  PubMed  CAS  Google Scholar 

  • Rister M, Baehner RL (1977) Effects of hyperoxia on Superoxide anion and hydrogen peroxide production of polymorphonuclear leukocytes and alveolar macrophages Br J Haemat 36:241–248

    Article  CAS  Google Scholar 

  • Rister M, Baehner RL (1978) Neue Aspekte zur Toxizität des Sauerstoffs. Dtsch Med Wochenschr 103:257–267

    Article  Google Scholar 

  • Rister M (1980a) Phagocytosemechanismen alveolärer Makrophagen und Granulozyten. Blut 41:257–267

    Article  PubMed  CAS  Google Scholar 

  • Rister M, Brucke G, Wegener F, Gladtke E (1980b) Concanavalin A capping in polymorphonuclear leukocytes. Eur J Ped 135:153–160

    Article  CAS  Google Scholar 

  • Rister M, Haneke C (1980c) Therapie des Steinbrinck-Chediak-Higashi Syndroms. Klin Pädiat 192:19–24

    Article  CAS  Google Scholar 

  • Rister M (1982) Effects of hyperoxia on phagocytosis. Blut 45:157–166

    Article  PubMed  CAS  Google Scholar 

  • Ukena TE, Berlin RD (1972) Effect of colchicine and vinblastine on the topographical separation of membrane functions. J Exp Med 136:1–7

    Article  PubMed  CAS  Google Scholar 

  • Williams D, Boxer LA, Oliver JM, Baehner RL (1977) Cytoskeleton regulation of Concanavalin A capping in pulmonary alveolar macrophage. Nature 267:255–257

    Article  PubMed  CAS  Google Scholar 

  • Wilson L, Bamberg JR (1974) Interaction of drugs with microtubule proteins. Fed Proc 33:158–166

    PubMed  CAS  Google Scholar 

  • Winter PM, Smith G (1972) The toxicity of oxygen. Anesthesist 37:210–241

    Article  CAS  Google Scholar 

  • Zigmond SH, Hirsch JG (1973) Leukocyte locomotion and chemotaxis. J Exp Med 137:9

    Article  Google Scholar 

  • Zurier RB, Weissmann G, Hoffstein S, Kammerman S, Tai HH (1974) Mechanism of lysosomal enzyme release from human leukocytes. Clin Invest 53:297–309

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Universitäts-Kinderklinik Köln, Josef-Stelzmann-Str. 9, 5000, Köln 41, Federal Republic of Germany

    M. Rister & M. Vollmering

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  1. M. Rister
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  2. M. Vollmering
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Additional information

Supported by Deutsche Forschungsgemeinschaft Ri 275/5

This study contains data of a thesis submitted in partial fulfilment of the requirement for the degree of a Medical Doctor

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Rister, M., Vollmering, M. Concanavalin A distribution in polymorphonuclear leukocytes and alveolar macrophages during hyperoxia. Virchows Archiv B Cell Pathol 43, 179–187 (1983). https://doi.org/10.1007/BF02932955

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  • DOI: https://doi.org/10.1007/BF02932955

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