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Cloning and sequencing of a novel glutaryl acylase β-subunit gene ofPseudomonas cepacia BY21 from bioinformatics

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Abstract

Pseudomonas cepacia BY21 was found to produce glutaryl acylase that is capable of deacylating glutaryl-7-aminocephalosporanic acid (glutaryl-7-ACA) to 7-aminocephalosporanic acid (7-ACA), which is a starting material for semi-synthetic cephalosporin antibiotics. Amino acids of the reported glutaryl acylases from variousPseudomonas sp. strains show a high similarity (>93% identity). Thus, with the known nucleotide sequences ofPseudomonas glutaryl acylases in GenBank, PCR primers were designed to clone a glutaryl acylase gene fromP. cepacia BY21. The unknown β-subunit gene of glutaryl acylase from chromosomal DNA ofP. cepacia BY21 was cloned successfully by PCR. The β-subunit amino acids ofP. cepacia BY21 acylase (GenBank accession number AY948547) were similar to those ofPseudomonas diminuta KAC-1 acylase except that Asn408 ofP. diminuta KAC-1 acylase was changed to Leu408.

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Authors and Affiliations

  1. Department of Biotechnology, Yeungnam University, 712-749, Gyeongsan, Korea

    Yoo-Seok Jeong

  2. Department of Applied Microbiology, Yeungnam University, 712-749, Gyeongsan, Korea

    Hyo-Jin Yoo, Sang-Dal Kim & Yong-Ho Khang

  3. Department of Pharmacy, Yeungnam University, 712-749, Gyeongsan, Korea

    Doo-Hyun Nam

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  1. Yoo-Seok Jeong
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  2. Hyo-Jin Yoo
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  3. Sang-Dal Kim
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  4. Doo-Hyun Nam
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  5. Yong-Ho Khang
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Correspondence to Yong-Ho Khang.

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Jeong, YS., Yoo, HJ., Kim, SD. et al. Cloning and sequencing of a novel glutaryl acylase β-subunit gene ofPseudomonas cepacia BY21 from bioinformatics. Biotechnol. Bioprocess Eng. 10, 510–515 (2005). https://doi.org/10.1007/BF02932286

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  • DOI: https://doi.org/10.1007/BF02932286

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