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Ordered expression of virulence genes inSalmonella enterica serovar typhimurium

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Abstract

Using transcriptional promoter fusions, we investigated the expression of selected SPI-1 and SPI-2 genes ofSalmonella enterica serovar Typhimurium (S. Typhimurium). Promoters of genes related to the invasion of the epithelial cell (hilA, hilC, hilD, invF, sicA, sopA, sopB andsopE2) were active in Luria-Bertani (LB) medium and LB with butyrate but were suppressed by bile salts and in glucose minimal (M9) medium. Genes related toS. Typhimurium intracellular survival (phoP, ssrA, ssaB, ssaG, sifA, sifB andpipB) were characterized by their expression in stationary phase in LB and M9 medium. Activity ofphoP andssrA promoters indicated that these might be expressed inside the gut. SPI-1 genes were expressed on the transition to stationary phase while SPI-2 genes were expressed in stationary phase. Among SPI-1 genes, those with regulatory functions preceded in expression the effector genes andsop genes were expressed in the order ofsopA, sopB andsopE2, showing hierarchy in the expression ofS. Typhimurium virulence genes.

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Abbreviations

LB:

Luria-Bertani (medium)

M9:

glucose minimum medium

RLU:

relative luminescence units

S. Typhimurium:

Salmonella enterica serovar Typhimurium

RT-PCR:

reverse transcription polymerase chain reaction

SPI:

salmonella pathogenicity island

TTSS:

type III secretion system

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Authors and Affiliations

  1. Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czechia

    K. Papezova, D. Gregorova, J. Jonuschies & I. Rychlik

Authors
  1. K. Papezova
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  2. D. Gregorova
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  3. J. Jonuschies
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  4. I. Rychlik
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Correspondence to I. Rychlik.

Additional information

This work has been supported by projects 1B44020 of theMinistry of Agriculture of the Czech Republic and EU project 505523.

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Papezova, K., Gregorova, D., Jonuschies, J. et al. Ordered expression of virulence genes inSalmonella enterica serovar typhimurium. Folia Microbiol 52, 107–114 (2007). https://doi.org/10.1007/BF02932148

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  • DOI: https://doi.org/10.1007/BF02932148

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