Abstract
Bacterial 16S rDNA from fecal samples of two calves were amplified by PCR and analyzed by denaturing gradient gel electrophoresis; selected bands were sequenced.Escherichia coli andBifidobacterium animalis were the initial colonizers, followed by species closely related to the generaBacteroides, Clostridium andFaecalibacterium. Change of diet was connected with shifts of bacterial population and with the occurrence of many bacterial species that have not been cultured up to now. The diet change corresponded with an alteration in a volatile-fatty-acid concentration in fecal samples.
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Abbreviations
- DGGE:
-
denaturing gradient gel electrophoresis
- PCR:
-
polymerase chain reaction
- VFA:
-
volatile fatty acids
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The study was supported by theGrant Agency of the Czech Academy of Sciences (project no. 1QS 5002 00572) and Institutional Research project of theInstitute of Animal Physiology and Genetics (AV 0Z 5045 0515).
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Lukáš, F., Koppová, I., Kudrna, V. et al. Postnatal development of bacterial population in the gastrointestinal tract of calves. Folia Microbiol 52, 99–104 (2007). https://doi.org/10.1007/BF02932147
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DOI: https://doi.org/10.1007/BF02932147