Abstract
Mutations extended the host range of the polyvalent bacteriophage 812 of the familyMyoviridae in up to 95 % ofStaphylococcus aureus strains and 43 % of strains of different coagulase-positive and -negativeStaphylococcus species. Mutational changes in the genome of several host-range mutants of phage 812 were identified. Host-range mutant 812F1 harbors a deletion in endolysin gene that arose together with intron excision. Four mutants (812i, 812b, 812p, 812F3) harbor deletion in the structural geneorf8 that results from a genome rearrangement associated with intron insertion. This rearrangement was also detected in the genome of the closely related phages U16 and ϕ131. Another intron was discovered in therecA812 gene in these four mutants. An insertion was found in a non-coding region of the restriction fragmentPstI-O of three mutants (812b, 812F3, 812g) and phages U16 and ϕ131. The above results contribute to the explanation of genetic factors affecting the host range of polyvalent staphylococcal bacteriophages.
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Abbreviations
- AFLP:
-
amplified fragment length polymorphism
- MALDI MS:
-
matrix-assisted laser desorption/ionization mass spectrometry
- RFLP:
-
restriction fragment length polymorphism
- RM:
-
restriction-modification (system)
- RT-PCR:
-
reverse transcription polymerase chain reaction
- SAM:
-
S-adenosylmethionine
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This work was supported by theCzech Science Foundation (grant no. 203/03/0515), long-term research program of theMinistry of Education, Youth and Sports of the Czech Republic (MŠM 00216 22415) and grant LSHM-CT-2006-019064 from theEuropean Union.
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Kašpárek, P., Pantůček, R., Kahánková, J. et al. Genome rearrangements in host-range mutants of the polyvalent staphylococcal bacteriophage 812. Folia Microbiol 52, 331–338 (2007). https://doi.org/10.1007/BF02932087
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DOI: https://doi.org/10.1007/BF02932087