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Effect of lactobacilli onE. coli adhesion to caco-2 cellsin Vitro

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Abstract

Inhibitory effect of various lactobacilli against pathogenic strains ofE. coli in model system Caco2 cells was determined by enumerating the number of adheringE. coli after pre-incubation (exclusion), post-incubation (displacement) or co-incubation (competition) with lactobacilli. PorcineE. coli strain F107 (F18ab, Stx2v) in the competition assay with porcine lactobacillus strain P10 gave bacterial counts 7.25 (log CFU per well); in the exclusion test it was only 7.05 while in displacement test it reached 7.29. The lowestE. coli counts adhering to Caco-2 cells were in exclusion assay (pre-incubation,Lactobacillus inoculated as the first). Pre-treatment ofE. coli with our lactobacilli strains reduced the cultivableE. coli numbers.

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Correspondence to K. Horošová.

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The project was supported byVEGA (grant no. 2/4001) and theSlovak Grant Agency (grant no. 2003 SP 51/028 09 00/028 09 06.

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Horošová, K., Bujňáková, D. & Kmeť, V. Effect of lactobacilli onE. coli adhesion to caco-2 cellsin Vitro . Folia Microbiol 51, 281–282 (2006). https://doi.org/10.1007/BF02931813

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  • DOI: https://doi.org/10.1007/BF02931813

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