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Production ofN-acylhomoserine lactone signal molecules by gram-negative soil-borne and plant-associated bacteria

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Abstract

Quorum-sensing control mediated byN-acylhomoserine lactone (AHL) signal molecules has been established as a key feature in the regulation of various metabolic traits in many bacteria. Approximately 300 strains representing 6 genera and 18 species of soil-borne and plant-associated Gram-negative bacteria isolated in various regions of the former USSR using two reporter systems were screened for AHL production. The production was observed in 17.5 % of the screened bacterial strains. Positive response was detected in all of the 14 tested strains ofErwinia herbicola, in 41 of the 239 strains ofPseudomonas species; in all 5 strains ofXanthomonas ampelina, X. campestris pv.malvacearum, pv.translucens, pv.vesicatoria and in one strain ofPantoea stewartii. AHL assay of 41 strains ofX. maltophilia (syn.Stenotrophomonas maltophilia) isolated from soils withChromobacterium violaceum reporter has revealed no strains synthesizing these signal molecules; 26 strains analyzed withAgrobacterium tumefaciens reporter showed the same result.

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Abbreviations

AHL:

N-acylhomoserine lactone

HHL:

N-hexanoylhomoserine lactone

HSL:

homoserine lactone

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Authors and Affiliations

  1. Institute of Molecular Genetics, Russian Academy of Sciences, 123 182, Moscow, Russia

    M. Vfselova, V. Lipasova, A. Metlitskaya, A. Mayatskaya & I. Khmel

  2. Institute of Genetics and Cytology, Belarus Academy of Sciences, 220 024, Minsk, Belarus

    M. Kholmeckaya, E. Voronina & E. Lobanok

  3. Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food, and Environmental Quality Sciences, The Hebrew University of Jerusalem, 76100, Rehovot, Israel

    S. Klein & L. Chernin

Authors
  1. M. Vfselova
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  2. M. Kholmeckaya
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  3. S. Klein
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  4. E. Voronina
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  5. V. Lipasova
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  6. A. Metlitskaya
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  7. A. Mayatskaya
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  8. E. Lobanok
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  9. I. Khmel
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  10. L. Chernin
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Corresponding author

Correspondence to I. Khmel.

Additional information

This research was supported in part by theSino-Israel Agricultural Research Fund (grant no. 823-0164-01), and by theRussian Foundation for Basic Research (grant no. 02-04-48514).

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Vfselova, M., Kholmeckaya, M., Klein, S. et al. Production ofN-acylhomoserine lactone signal molecules by gram-negative soil-borne and plant-associated bacteria. Folia Microbiol 48, 794–798 (2003). https://doi.org/10.1007/BF02931516

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  • DOI: https://doi.org/10.1007/BF02931516

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