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Identification and characterization of an endolysin encoded by theStreptomyces aureofaciens phage μ1/6

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Abstract

An open reading frame homologous to the genes encoding several cell-wall hydrolyzing enzymes was identified on the genome of actinophage μ1/6. This open reading frame encoding the putative endolysin was amplified by polymerase chain reaction and cloned into the expression vector pET-21a. This gene consisted of 1182 bp encoding a 393 amino acid polypeptide with a molar mass of 42.1 kDa. The gene product was overexpressed inEscherichia coli, and then the lytic enzyme was purified by a two-step chromatographic procedure. When applied exogenously, the endolysin of phage μ1/6 was active against all testedStreptomyces strains but did not affect other bacteria. The amino acid sequence showed a high homology with a putative amidase of theStreptomyces phage ΦC31. Downstream of the endolysin gene, an open reading frame encoding an 88 amino acid protein was identified. Structural analysis of its sequence revealed features characteristic for holin.

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Authors and Affiliations

  1. Institute of Molecular Biology, Slovak Academy of Sciences, 845 51, Bratislava, Slovakia

    J. Farkašovská & A. Godány

  2. Center for Integrated Genomics, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, 166 37, Pragae, Czechia

    Č. Vlček

Authors
  1. J. Farkašovská
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  2. A. Godány
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  3. Č. Vlček
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This work was supported by the VEGA grant no. 2/2059/22 of theSlovak Academy of Sciences.

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Farkašovská, J., Godány, A. & Vlček, Č. Identification and characterization of an endolysin encoded by theStreptomyces aureofaciens phage μ1/6. Folia Microbiol 48, 737–744 (2003). https://doi.org/10.1007/BF02931507

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  • DOI: https://doi.org/10.1007/BF02931507

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