Abstract
Flowering in the short day plantChenopodium rubrum was inhibited by 5-fluoro-deoxyuridine (FDU) at a concentration of 4×10−6 M and higher when applied during photoperiodic induction or immediately afterwards. This inhibition is always accompanied by a general reduction of growth (e.g. a decrease in the first leaf length). The mitotic activity within the shoot apex is completely blocked by FDU application during the photoperiodic treatment. The floral induction (evocationsensu Evans) was not cancelled in this situation as was revealed when reversing the FDU effect by thymidine application. One day after the end of the photoperiodic treatment (the plants were transferred to continuous light again) the FDU inhibition becomes irreparable. The results indicate that DNA synthesis and hence the mitotic activity are not obligatory prerequisites for photoperiodic floral induction inChenopodium. Low concentrations of FDU may promote flowering under suboptimal floral induction.
Abstract
Fluor-5-deoxyuridin (FUDR) v koncentraci 4×10−6 M a vyšší inhibuje kvetení uChenopodium rubrum při aplikaci v průběhu fotoperiodické indukce nebo po ní. Tato inhibice je vždy provázena i celkovým potlačením růstu (např. délky listů). Přítomnost FUDRu v apikálních meristémech v průběhu fotoperiodického zásahu vede k úplnému potlačení mitotické aktivity, aniž zabránila indukei, jak o tom svědčí pokusy s reverzí dlouhodobé inhibice FUDRu thymidinem. 24 hodiny po převedení rostlin z indukčních podmínek (krátkého dne) na nepřetržité osvětlení je již inhibice vyvolaná FUDRem irreverzibilní. Výsledky ukazují, že mitotická aktivita a syntéza nové DNK nejsou podmínkami pro fotoperiodickou květní indukei uChenopodium rubrum. V suboptimálních podmínkách indukee mohou nižší koncentrace FUDRu vyvolat kvetení.
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Ullmann, J., Seidlová, F. & Krekule, J. 5-Fluorodeoxyuridine inhibition of photoperiodically induced flowering inChenopodium rubrum L.. Biol Plant 13, 305–312 (1971). https://doi.org/10.1007/BF02930917
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DOI: https://doi.org/10.1007/BF02930917