Abstract
Multiple shoots (16–20 shoots per expiant) were induced from cotyledonary node region ofAnogeissus acuminata (Roxb. ex DC.) Guill. & Perr. on Murashige and Skoog’s (MS) medium containing IAA 0.1 mg 1-1 + BAP 1.5 mg 1-1 and ascorbic acid 50 mg 1-1, citric acid 25.0 mg 1-1, arginine 25 mg 1-1 and adenine sulphate 25 mg 1-1. From the first node of seedling only 4–6 shoots per expiant were proliferated. Segments ofin vitro produced shoots were used as expiants for further multiplication of shoots upto 16 successive cultures at an interval of 4 week on MS medium with IAA 0.1 mg 1-1 + BAP 1.0 mg 1-1 and additives. The original cotyledonary expiant was repeatedly subcultured upto 4 times after harvesting crop of shoots, each time.In vitro produced shoots were rooted on half strength MS medium containing 0.5 mg 1-1 IBA. Plantlets were transferred to pots. Other expiants (cotyledons, hypocotyl, and leaf) produced callus on medium containing auxins and cytokinins. The calluses differentiated into embryo like structures or roots on MS medium.
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Abbreviations
- BAP:
-
benzylaminopurine
- IBA:
-
indolebutyric acid
- NAA:
-
naphtaleneacetic acid
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
References
Aitken-Christie, J., Joncs, C.: Towards automation: Radiata pine shoot hedgesin vitro. - Plant Cell Tissue Organ Cult.8: 185–196, 1987.
Boulay, M.: Some practical aspects and applications of the micropropagation of forest trees. - In: Proceedings of the International Symposium on “Invitro Propagation of Forest Tree Species”. Pp. 51–81. A.R.F., Bologna 1985.
Chalupa, V.: Large scale micropropagation ofQuercus robur L. using adenine-type cytokinins and thidiazuron to stimulate shoot proliferation. - Biol. Plant.30: 414–421, 1988.
Chalupa, V.: Micropropagation of hornbeam(Carpinus betulus L.) and ash(Fraxinus excelsior L). - Biol. Plant.32: 332–338, 1990.
Gaspar, T., Coumans, M.: Root formation. - In: Bonga, J.M., Durzan, D.J. (ed.): Cell and Tissue Culture in Forestry. Volume 2. Pp. 202–217. Martinus Nijhoff Publishers, Dordrecht - Boston - Lancaster 1987.
Joshi, R., Shekhawat, N.S., Rathore, T.S.: Micropropagation ofAnogeissus pendula Edgew. - an arid forest tree. - Indian J. exp. Biol.29: 615–618, 1991.
Joy, R.W., Patel, K.R., Thorpe, T.A.: Ascorbic acid enhancement of organogenesis in tobacco callus. - Plant Cell Tissue Organ Cult.13: 219–228, 1988.
Krikorian, AD.: Plant tissue culture: Perceptions and realities. - Proc. Indian Acad. Sci. Plant Sci.98: 425–464, 1988.
Murashige, T. Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. - Physiol. Plant.15: 473–497, 1962.
Rathore, T.S., Singh, R.P., Shekhawat, N.S.: Clonal propagation of desert teak (Tecomella undulata) through tissue culture. - Plant Sci.15: 217–222, 1991.
Roy, S.K., Pal, R.K., Das, S,K.: Propagation of a timber tree,Terminalia bellerica Roxb. by tissue culture. - Bangladesh J. Bot.16: 125–130, 1987.
Sharma, A.K., Chaturvedi, H.C.: Micropropagation ofBougaivillea xButtiana Scarlet queen variegated by shoot tip culture. - Indian J. exp. Biol.26: 285–288, 1988.
Thorpe, T.A.: Application of tissue culture technology to forest tree improvement. - Forest. Chronicle61:436–438, 1985.
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Rathore, T.S., Deora, N.S., Shekhawat, N.S. et al. Rapid micropropagation of a tree of arid forestryAnogeissus acuminata . Biol Plant 35, 381–386 (1993). https://doi.org/10.1007/BF02928513
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DOI: https://doi.org/10.1007/BF02928513