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Drosophila “enhancer-trap” transposants: Gene expression in chemosensory and motor pathways and identification of mutants affected in smell and taste ability

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Abstract

We have isolated about a thousandDrosophila P-element transposants that allow thein situ detection of genomic enhancer elements by a histochemical assay for β-galactosidase activity. We summarize the β-galactosidase staining patterns of over 200 such transposants in the adult. Our aim was to identify genes that are likely to be involved in the chemosensory and motor pathways ofDrosophila. Based on β-galactosidase expression patterns in the tissues of our interest, we have chosen some strains for further analysis. Behavioral tests on a subset of the transposants have, in addition, identified several strains defective in their chemosensory responses.

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References

  • Arora K., Rodrigues V., Joshi S. and Siddiqi O. 1987 A gene affecting the specificity of chemosensory neurons ofDrosophila.Nature (London) 330: 62–63

    Article  CAS  Google Scholar 

  • Ashburner M. 1989Drosophila: A laboratory handbook (Cold Spring Harbor, NY: Cold Spring Harbor Laboratory)

    Google Scholar 

  • Ayyub C., Paranjape J., Rodrigues V. and Siddiqi O. 1990 Genetics of olfactory behavior inDrosophila melanogasier. J. Neurogenet. (in press)

  • Ballinger D. D. G. and Benzer S. 1989 Targeted gene mutations inDrosophila.Proc. Natl. Acad. Sei. USA. 86: 9402–9406

    Article  CAS  Google Scholar 

  • Bellen H. J., O’Kane C. J., Wilson C., Grossniklaus U., Pearson R. K. and Gehring W. J. 1989a P-element mediated enhancer detection: a versatile method to study development inDrosophila.Genes Dev. 3: 1288–1300

    Article  PubMed  CAS  Google Scholar 

  • Bellen H. J., Wilson C. and Gehring W. J. 1989b Dissecling the complexity of the nervous system by enhancer detection.BioEssays 12: 199–205

    Article  Google Scholar 

  • Bier E., Vaessin H., Shepherd S., Lee K., McCall K., Barbel S., Ackerman L., Caretto R., Uemura T., Grell E., Jan L. Y. and Jan Y. N. 1989 Searching for pattern and mutation in theDrosophila genome with a P-lacZ vector.Genes Dev. 3: 1273–1287

    Article  PubMed  CAS  Google Scholar 

  • Campos-Ortega J. A. and Harlcnstein V. 1985The embryonic development of Drosophila melanogasler (Berlin: Springer)

    Google Scholar 

  • Cooley L., Kelley R. and Spradling A. 1988 Insertional mutagenesis of theDrosophila genome with single P-elements.Science 239: 1121–1128

    Article  PubMed  CAS  Google Scholar 

  • Coslello W. J. and Wyman R. J. 1986 Development of an indirect flight muscle in a muscle-specific mutant ofDrosophila melanogaster.Dev. Biol. 118: 247–258

    Article  Google Scholar 

  • Crossley A. C. 1978 The morphology and development of theDrosophila muscular system. InGenetics and biology of Drosophila (eds.) M Ash Burner and T R F Wright (New York: Academic Press) vol. 2b, pp. 499–560

    Google Scholar 

  • Deak I. I., Bellamy P. R., Bienz M., Dubuis Y., Fenner E., Gollin M., Rahmi A., Ramp T., Reinhardt C. A. and Cotton B. 1982 Mutations affecting the indirect flight muscles inDrosophila melanogaster.J. Embryol. Exp. Morphol 69: 61–81

    PubMed  CAS  Google Scholar 

  • Engels W. R. 1988 P-elemcnts inDrosophila. InMobile DNA (eds) D. E. Berg and M. M. Howe (Washington, DC: American Society for Microbiology)

    Google Scholar 

  • Fasano L. and Kerridge S. 1988 Monitoring positional information during oogenesis in adultDrosophila.Development 104: 245–253

    PubMed  CAS  Google Scholar 

  • Grossniklaus U., Bellen H. J., Wilson C. and Gehring W. 1989 P-element mediated enhancer detection applied to the study of oögenesis inDrosophila.Development 107: 189–200

    PubMed  CAS  Google Scholar 

  • Hamilton B. A., Palazzolo M. J., Chang J. H., Vijay Raghavan K., Mayeda C. A., Whitney M. A. and Meyerowitz E. M. 1990 Large scale detection of transposon insertions into cloned loci by plasmid rescue of flanking DNA. (submitted for publication)

  • Kaiser K. and Goodwin S. F. 1990 “Site selected” transposon mutagenesis ofDrosophila.Proc. Natl. Acad. Sci. USA. 87: 1686–1690

    Article  PubMed  CAS  Google Scholar 

  • Langer-Safer P. R., Levine M. and Ward D. C. 1982 Immunocytochemical method for mapping genes onDrosophila polytene chromosomes.Proc. Natl. Acad. Sci. USA 79: 4381–4385

    Article  PubMed  CAS  Google Scholar 

  • Lawrence P. A. 1982 Cell lineage of the thoracic muscles ofDrosophila.Cell. 29: 493–503

    Article  PubMed  CAS  Google Scholar 

  • Lindsley D. L. and Grell E. H. 1968Genetic variations of Drosophila melanogaster (Washington DC: Carnegie Institute of Washington)

    Google Scholar 

  • McKenna M., Monte P., Helfand S. L. and Carlson J. R. 1989 A simple chemosensory response and the isolation ofacj mutants in which it is affected.Proc. Natl. Acad. Sci. USA 86: 8118–8112

    Article  PubMed  CAS  Google Scholar 

  • Nayak S. V. and Singh R. N. 1983 Sensilla on the tarsal segments and mouthparts of adultDrosophila melanogaster.Int. J. Insect Morphol. Embryol. 12: 273–291

    Article  Google Scholar 

  • Nayak S. V. and Singh R. N. 1985 Primary sensory projections from the labella to the brain ofDrosophila melanogaster Meigen (Diplera: Drosophilidiae).Int. J. Insect Morphol. Embryol. 14: 115–129

    Article  Google Scholar 

  • O’Kane C. J., and Gehring W. J. 1987 Detectionin situ of genomic regulatory elements inDrosophila.Proc. Natl. Acad. Sci. USA 84: 9123–9127

    Article  PubMed  CAS  Google Scholar 

  • Palazzolo M. J., Hyde D., Vijay Raghavan K., Mecklenburg K., Benzer S. and Meyerowitz E. M. 1989 Use of a new strategy to isolate and characterize 436Drosopliila cDNA clones corresponding to RNAs detected in adult heads but not in early embryos.Neuron 3: 527–539

    Article  PubMed  CAS  Google Scholar 

  • Palazzolo M. J., Hamilton B. A., Ding D., Martin C. H., Mead D. A., Mierendorf R. C., Vijay Raghavan K., Meyerowitz E. M. and Lipshitz H. D. 1990 Lambda phage cDNA cloning vectors for subtractive hybridization, fusion protein expression and Cre-loxP automatic plasmid subcloning.Gene 88: 25–36

    Article  PubMed  CAS  Google Scholar 

  • Pinto L., Stocker R. F. and Rodrigues V. 1988 Anatomical and neurochemical classification of the antennal glomeruli inDrosophila melanogaster Meigen (Diptera: Drosophilidae).Int. J. Insect. Morphol. Embryol. 17: 335–344

    Article  Google Scholar 

  • Pirrotta V. 1986 CloningDrosphila genes. InDrosophila, a practical approach (ed) D. R. Roberts (Oxford, Washington DC: IRL press) pp. 83–110

    Google Scholar 

  • Robertson H. M., Preston C. R., Phillis R. W., Johnson-Schlitz D. M., Benz W. K. and Engels W. R. 1988 A stable source of P-element transposase inDrosophila.Genetics 118: 461–470

    PubMed  CAS  Google Scholar 

  • Rodrigues V. 1988 Spatial coding of olfactory information in the antennal lobe ofDrosophila melanogaster.Brain Res. 453: 299–307

    Article  PubMed  CAS  Google Scholar 

  • Rodrigues V. and Siddiqi O. 1978 Genetic analysis of chemosensory pathway.Proc. Indian Acad. Sci. B87: 147–160

    Google Scholar 

  • Sambrook J., Fritsch E. F. and Maniatis T. 1989Molecular cloning: A laboratory manual 2nd edn (Cold Spring Harbor, NY: Cold Spring Harbor Laboratory)

    Google Scholar 

  • Serfling E., Jasin M. and Schaffner W. 1985 Enhancers and eucaryotic gene transcription.Trends Genet. 1: 224–230

    Article  CAS  Google Scholar 

  • Siddiqi O. 1987 Neurogenetics of olfaction inDrosophila melanogaster.Trends Genet. 3: 137–142

    Article  CAS  Google Scholar 

  • Tanimura T., Isono K., Takamura T. and Shimada I. 1982 Genetic dimorphism in taste sensitivity to trehalose inDrosophila melanogaster.J. Comp. Physiol. 147: 433–437

    Article  Google Scholar 

  • Vijay Raghavan K., Crosby M. A., Mathers P. H. and Meyerowitz E. M. 1986 Sequences sufficient for correct regulation ofSgs-3 lie close to or within the gene.EMBO. J. 5: 3321–3326

    Google Scholar 

  • Vijay Raghavan K., Palazzolo M. J. and Rodrigues V. 1990 Molecular and reverse genetic methods of studying complex genomes: TheDrosophila nervous system as a model.Curr. Sci. (in press)

  • Wilson C., Pearson R. K., Bellen H. J., O’Kane C. J., Grossniklaus U. and Gehring W. J. 1989a P-element mediated enhancer detection: an efficient method for isolating and characterizing developmentally regulated genes inDrosophila.Genes Dev. 3: 1301–1313

    Article  PubMed  CAS  Google Scholar 

  • Wilson C., Bellen H. J., Pearson R. K., O’Kane C. J., Engstrom Y., Gibson G., Grossniklaus U. and Gehring W. 1989b The little biue book.Drosoph. Info. Serv. 69 (in press)

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Author notes

  1. M. C. Arunan

    Present address: Sophia College for Women, B. Desai Road, 400026, Bombay, India

Authors and Affiliations

  1. Microbiology and Cell Biology Department, Indian Institute of Science, 560012, Bangalore, India

    Anuranjan Anand

  2. Molecular Biology Unit, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, 400005, Bombay, India

    Joyce Fernandes, Savita Bhosekar, Abha Chopra, Kevin Sequiera, Gaiti Hasan, K. Vijay Raghavan & Veronica Rodrigues

  3. Division of Chemistry and Chemical Engineering, California Institute of Technology, 91125, Pasadena, California, USA

    Neilay Dedhia

  4. Department of Genetics, Washington University Medical School, 63110, St. Louis, Mo., USA

    Michael J. Palazzolo

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  1. Anuranjan Anand
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Anand, A., Fernandes, J., Arunan, M.C. et al. Drosophila “enhancer-trap” transposants: Gene expression in chemosensory and motor pathways and identification of mutants affected in smell and taste ability. J. Genet. 69, 151–168 (1990). https://doi.org/10.1007/BF02927976

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  • DOI: https://doi.org/10.1007/BF02927976

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