Abstract
The rate of protein synthesis inStreptomyces aureofaciens, measured by incorporation ofU-14C-l-leucine into cells, fluctuated during the production phase in the range of 10–15 % of the values determined in the phase of intensive growth. Tetracycline partially inhibited the protein synthesis during the growth phase only. The proteins synthesized between the 6th and 18th hour of growth, were 75 % degraded by the 48th hour. The DNA synthesis, measured by means of incorporation of 2-14C-thymine into the mycelium, occurred predominantly during the first 24 h of cultivation. Similarly, DNA synthesized between the 6th and 12th hour of cultivation was degraded by 75 % after 48 h. The turnover of culture proteins is thus caused largely by degradation of old cells and growth of new ones which are more resistant to tetracycline. The activity of alanine aminotransferase and aspartate aminotransferase increase substantially towards the end of fermentation.
Similar content being viewed by others
References
Běhal V., Jechová V., Vaněk Z., Hošťálek Z.: Alternate pathways of malonylCoA formation inStreptomyces aureofaciens. Phytochem.16, 347 (1977).
Gräfe U., Bocker H., Reinhard G., Thrum H.: Regulative Beeinflussung der Nourseothricinbiosynthese durch α-Aminobenzoesäure in Kultur desStreptomyøes noursei JA 3890b.Z. Allg. Mikrobiologie 14, 659 (1974).
Hošťálek Z.: Relationship between the carbohydrate metabolism ofStreptomyces aureofaciens and the biosynthesis of chlortetracycline II. The effect of benzylthiocyanate on the respiration of washed mycelium ofStreptomyces aureofaciens.Folia Microbiol. 9, 78 (1964).
Ludvík J., Mikulík K., Vakěk Z.: Fine structureStreptomyces aureofaciens producing tetracycline.Folia Microbiol. 16, 479 (1971).
Mikulík K., Karnetová J., Quyen N., Blumauerová M., Komersová I., Vaněk Z.: Interaction of tetracycline with protein synthesizing system ofS. aureofaciens.J. Antib. 24, 81 (1971).
Sitzer W. I., Jenkins W. T.: Glutamio aspartic transaminase from pig ventricles.Methods Enzymol. 5, 611 (1961).
Timko J., Sabo B., Zelinka J.: Nucleic acids and ribosomes from mycelium of Streptomyøesaureofaciens.Biológia 31, 703 (1976).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Běhal, V., Vaněk, Z., Hošťálek, Z. et al. Synthesis and degradation of proteins and DNA inStreptomyces aureofaciens . Folia Microbiol 24, 211–216 (1979). https://doi.org/10.1007/BF02926450
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02926450