Abstract
In isolated mixture of exocellular enzymes of rumen bacteriaRuminococcus flavefaciens, Butyrivibrio fibrisolvens and rumen fungusNeocallimastix frontalis, specific activities of cellulases, hemicellulases and glycosidases were determined. The highest specific activities were shown mostly for proteins ofN. frontalis.
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References
Ben-Shalom M.: Hindrance of hemicellulose and cellulose hydrolysis by pectic substances.J. Food Sci.51, 720–730 (1986).
Chesson A., Stewart C.S., Dalgaro K., King P.R.: Degradation of isolated grass mesophyll, epidermis and fibre cell-walls in the rumen and by cellulolytic rumen bacteria in exenic culture.J. Appl. Bacteriol.60, 327–336 (1986).
Coleman G.S.: Cellulase content of 15 species of entodiniomorphid protozoa, mixed bacteria and plant debris isolated from the bovine rumen.J. Agric. 8oi. Camb.104, 349–360 (1985).
Dehority B.-A.: Hemicellulose degradation by rumen bacteria.Feder. Proc.32, 1819–1825 (1973).
Deshpande M.V., Eriksson K.E., Pettersson L.G.: An assay for selective determination of exo-1,4-β-glucanases in a mixture of cellulolytic enzymes.Anal. Biochem.138, 481–487 (1984).
Greve L.C., Labavitch J.M., Hungate B.E.:α-1-Arabinofuranosidase fromRuminococcus albus 8: purification and possible role in hydrolysis of alfalfa cell-wall.Appl. Environ. Microbiol.47, 1135–1140 (1984).
Holdeman L.V., Cato E.P., Moore W.E.C.:Anaerobe Laboratory Manual, 3rd Ed. Virginia Polytechnic Institute and State University, Blackburg (Virginia) 1977.
Lever M.: Carbohydrate determination with 4-hydroxybenzoic acid hydrazide (POHBAN): Effect of bismuth on the reaction.Anal. Biochem.81, 21–27 (1977).
Richardson A.J., Stewart C.S., Cambell G.P., Wilson A.B., Joblin K.N.: Influence of coculture with rumen bacteria on the ligno-cellulolytic activity of phycomycetous anaerobic fungi from the rumen. XlVth Int.Congr.Microbiol., Microbe 86, Manchester 1986.
Saddler J.N.: Factors limiting the efficient of cellulase enzymes.Microbiol. Sci.3, 84–87 (1986).
Wallace J.K., Brammall M.L.: The role of different species of bacteria in the hydrolysis of protein in the rumen.J. Gen. Microbiol.131, 821–832 (1985).
Wilkie K.C.B.: The hemicelluloses of grasses and cereals.Adv. Carb. Chem. Biochem.36, 215–264 (1979).
Williams A.G., Strachan N.H.: The distribution of polysaccharide-degrading enzymes in the bovine rumen digesta ecosystem.Curr. Microb.10, 215–220 (1986).
Williams A.G., Withers S.E.: The production of plant cell-wall polysaccharide-degrading enzymes by hemicellulolytic rumen bacterial isolates grown on a range of carbohydrate substrates.J. Appl. Bacteriol.52, 377–378 (1982).
Wolin M.J., Miller T.L.: Interactions of microbial populations in cellulose fermentation.Fed. Proc.42, 109–113 (1983).
Wong K.K.Y., Tan L.U.L., Saddler J.N.: Functional interactions among three xylanases fromTrichoderma harzianum.Enzyme Microb. Technol.8, 617–622 (1986).
Wood T.M., McCrae S.I.: Synergism between enzymes involved in the solubilization of native cellulose.Adv. Chem. Ser.181, 181–209 (1979).
Wood T.M., Wilson C.A., McRae S.I., Joblin K.N.: A highly active extracellular cellulase from the anaerobic rumen fungusNeocallimastix frontalis.FEMS Microbiol. Lett.34, 37–40 (1986).
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Kopečný, J., Williams, A.G. Synergism of rumen microbial hydrolases during degradation of plant polymers. Folia Microbiol 33, 208–212 (1988). https://doi.org/10.1007/BF02925906
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DOI: https://doi.org/10.1007/BF02925906